多染色性

Methods: Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis-ethylene dibromide (PCR-PAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计 12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳-EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Methods: Twelve microsatellite markers located at chromosomes 3p, 9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis ethylene dibromide (PCR-FAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳－EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Here is an adrenal cortical carcinoma seen microscopically at high power to demonstrate cellular pleomorphism with nuclear hyperchromatism.

高倍镜下的肾上腺皮质癌，显示细胞的多形性、细胞核染色过深。

Occasionally, lipoblasts can be seen in lipomas, especially near blood vessels. However, they do not exhibit cytologic atypia/hyperchromasia/pleomorphism.

少数情况下，脂肪母细胞也能在脂肪瘤中看到，特别是近血管区域，但是，它们不显示细胞异型/深染色质/多形性。

Results Positive rate of Amsel was 28%, one step sialidase was 34%, Gram-stain was 30% and amines was 24%. Using Amsel as criterion, the sensitivity of one step sialidase and amines was 92.9% and 71.4%; specificity was 88.9% and 94.4%; positive predictive value was 76.5% and 83.3% negative predictive value was 97% and 89.5%, using Gram-stain method as criterion, the sensitivity of one step sialidase and amines was 93.8% and 66.7%, specificity was 92.1% and 94.3%, positive predictive value was 83.3% and 83.3%, negative predictive value was 97.2% and 86.8% respectively.

Amsel法阳性率28%，组织多胺试验阳性率24%，一步法唾液酸酶活性的检测试验阳性率34%，革兰染色细菌评分法阳性率30%；唾液酸酶活性检测法与Amsel法，革兰染色细菌评分法相比较差异无显著性；组织多胺试验与Amsel法，革兰染色细菌评分法相比较差异无显著性；以Amsel法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为92.9%和71.4%，特异性分别为88.9%和94.4%，阳性预期值分别为76.5%和83.3%，阴性预期值分别为97.0%和89.5%；以革兰染色细菌评分法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为93.8%和66.7%，特异性分别为92.1%和94.3%，阳性预期值都为83.3%，阴性预期值分别为97.2%和86.8%。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型，胞质丰富，胞核成圆形或椭圆型，核仁清楚，甲苯胺蓝呈异染性，阿尔新蓝8Gx 染色阳性，细胞外基质Ⅱ型胶原免疫组化染色阳性，Ⅰ型胶原染色阴性。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

Methods We reported a case of PXA located in the cerebellum of a young adult in view of its extremely unusual location. Morphologic observation, immunohistochemical staining using DAKO EnVision system , histochemical stains including hematoxylin eosin and Gordon Sweet's reticulin methods were used.

采用免疫组织化学DAKOEnVison法、组织化学网质纤维染色和HE染色及形态学观察的方法，报道 1例极罕见于年轻人小脑的多形性黄色星形细胞瘤，探讨其临床病理特点。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

In this paper, The influence of heat set and dyeing on the structure of PTT fiber was investigated by FT-IR, DSC, critical dissolution time. The influence of heat set on the dyeability of PTT fiber was discussed, too. Meanwhile, many experiments on the dyeing performance of PET fiber were carried out. The effect of different dyeing temperature on the uptake and visual color depth value and dyeing characteristics of different dyes with temperature increasing were discussed, and the influence of temperature on the dyeing depth were also evaluated when dyeing for PTT and PET fiber in the same dye bath condition. Especially the kinetics and thermodynamics of dyeing with disperse dyes were mainly studied.

目前，我国对PTT染色性能方面研究报道还少，本文用傅立叶红外光谱、差热分析、临界溶解时间等分析方法研究了热定型和染色对PTT纤维结构的影响，探讨了热定型对PTT纤维可染性的影响，同时进行了多项PTT纤维的基本染色性能试验，探讨了不同染色温度对上染百分率及表观色深值的影响、不同染料的升温上染特性、不同染料的移染性能和染深性能以及PTT和PET纤维同浴染色时染色温度对它们的染色深度的影响。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。