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It is suggested that it is better to indicate the variability within breeds by average heterozygosity based on microsatellite polymorphisms, which showed high polymorphisms and codominant inheritance of the markers, where as differences between populations and even specific characteristics with in a population are reffected clearly and directly by RAPD.

由于微卫星标记多态性程度高且等显性遗传,因而通过它计算杂合度可以较好地反映群体内的变异;RAPD则在反映群体间的区别甚至某一群体的特征时可提供明确而直接的信息。

The results based in microsatellite polymorphisms showed that the average heterozygosity in Huiyang Bearded and Xinghua chickens which were sampled from conserver populations, and Yuehuang which has been inbreeding for more than 10 years was low, buti n Qingyuan Partridge chicken which was sampled from Qingyuan city, Guangdong, was high.

用5个座位的微卫星标记和70个10碱基引物得出的RAPD,研究分析了惠阳胡须鸡、杏花鸡和清远麻鸡3个广东地方鸡种和培育品种粤黄鸡的群体遗传变异及相互间的关系,并讨论了微卫星多态性和RAPD这两种标记在应用于群体遗传变异及亲缘关系等方面分析的意义。

Specifically, you select which type of database you want to call and the code will use the principles of polymorphism to call the specific database class.

非常明确的,当你选择你需要调用的哪种类型的数据库时,代码会使用多态性的规则来调用准确的数据库类。

Methods: Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis-ethylene dibromide (PCR-PAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计 12个微卫星多态性标记,采用PCR-中性聚丙烯酰胺凝胶电泳-EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Methods: Twelve microsatellite markers located at chromosomes 3p, 9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis ethylene dibromide (PCR-FAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计12个微卫星多态性标记,采用PCR-中性聚丙烯酰胺凝胶电泳-EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

The resulsts indicated that:there was a great difference in the pollen shape of male and female Vitis, the pattern of SOD isozyme were the same in male and female dioecism Vitis; some of the results of RAPD were polymorphic between the male and female.

结果表明:葡萄雌雄株的花粉形态存在着较大的差异;在本实验条件下葡萄雌雄株叶片SOD同工酶谱带一致;一些引物的RAPD产物间存在着多态性,在葡萄雌雄株的基因组DNA序列中存在着碱基排列顺序的差异。

The purpose of the research is: 1 to make the distinguishment of these hybrid rice combinations and their parents; 2 to establish a set of screening pictures and DNA fingerprint map concerning these super hybrid rice combinations and their parents; 3 to find a convenient method for identification of rice seed purity.In this study, 144 SSR primer pairs distributing in 12 rice chromosomes were used to detect the experimental materials.

本研究通过SSR分子标记技术分析进行超级杂交稻先锋和苗头组合的鉴定,获得以下结果:(1)对分布于水稻1-12号染色体的144对SSR引物进行了筛选,有47对能够在实验材料中显示较好的多态性通过,占检测引物的32.6%。

To study the efficacity of space-induced towards the Artemia sinica spawn and select individuals with big genetic differences to propagate,this paper collect totally 59 space-induced and control Artemia sinic to carry out the genetic diversity inspection and relative's analyses using four sets of AFLP selective primers for amplification.

每对引物均获得清晰的扩增图谱,共检出222个清晰可辨的不同扩增片段,其中多态位点189个,占85.1%;UPGMA和NJ聚类分析图显示,来自空间诱变组的M5单独聚类,而其它58个个体聚为另一类,其他太空诱变组与地面对照组的个体交叉聚类,地面对照和太空诱变组遗传差异不明显。

Because if the more profitable site for SMEs, China's Internet industry will explode into a state of the speed of the development of even stronger.

因为假如更多的中小企业站点实现赢利,中国的互联网产业才会成爆炸态的速度发展的更加壮大。

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛,包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等,已广泛应用于医学临床,它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

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