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While doing the RFLP tagging of giant embryogene,the genome changebetween mutants and its original parent,the relationship between RFLP andheteroses in indica,japonica and intermediate rice,the performance of multipleand null allelism in rice varieties,the utilization of null allelic RFLP probes inidentifying indica and japonica rice specifically,the distribution of the nullallelic RFLP probe RG684 in rice cultivars,and the segregations of RFLPmakers in indica/japonica F2 population were also studied.

本实验从经典遗传、分子遗传、育种潜力评估、育种新品系选育等领域,对巨大胚、甜胚乳、暗胚乳三种新型突变体进行了全面系统研究。在对巨大胚进行分子定位过程中,对涉及到的突变体与原始亲本基因组变化,籼、粳、中间型材料的RFLP多态性与杂种优势之间关系,水稻品种的复等位、零等位表现,鉴定籼、粳特异性的零等位RFLP标记,零等位标记RG684在稻种中分布,RFLP标记在籼粳交F2群体中分离等遗传现象也一并进行了分析。

Nigra, B. oleracea and B. carinata belonged to class 2A according to the classification of karyotypes by Stebbins (1971). According to Levitzky's and Stebbin's view, the more asymmetric karyotype a species has, the more evolved it is. Therefore, B. campestris is the furthest evolved species among the elementary species, and B. juncea and B. napus the furthest among the amphidiploid species. Compared with the previous reports, there is no any cytoplasm in the chromosome sample prepared using this protocol which is very useful for the karyotype analysis, as well as the FISH research on Brassica species.

对上述研究结果分析表明:(1)芸薹属复合种是由基本种相互杂交而成;(2)B基因组与A或与C基因组亲缘关系较远,而A和C基因组亲缘关系较近;(3)芸薹属复合种在演化过程中,发生了染色体结构上的重排现象;(4)芸薹属复合种演化过程中,rRNA基因位点发生了减少;所获结果与国外同类报道相比表明:国内的白菜型油菜存在新的rRNA基因位点,即rRNA基因在芸薹属同一物种不同的品种间具有多态性;有关这类研究国内尚未见报道。

By using the sliding mode approach and a multi-step control strategy, a new controller is presented, which can not only satisfy the control constraints but also stabilize the system to an arbitrarily small ε-neighborhood about its equilibrium in a finite time.

利用滑动模态的思想和多步控制策略,设计了不连续反馈镇定律,该镇定律一方面满足控制受限条件,另一方面使得闭环系统的状态收敛到预先给定的原点的任意小的ε邻域中。

We studied polymorphisms of 23 microsatellite loci from 23 chromosomes in an indigenous breed of Xuefeng black bone chicken from Hunan in order to elucidate its genetic diversity and structure and to propose reasonable measures for its conservation and utilization.

为了评价雪峰乌骨鸡现有品种资源的遗传背景、群体遗传结构等,提出合理的保种方法和措施,本研究筛选了家鸡基因组23条染色体上的23个微卫星标记,对采自湖南怀化的50只纯种乌骨鸡个体进行了多态性检测。

According to the dendrogram by UPGMA cluster analysis using Neis(1972) distance calculations based on AFLP banding, all the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Cajanus cajan. From ME cluster analysis, 9 distinct evolutionary groups were detected. The preliminary results indicated that China maybe one of the pigeonpea genetic diversity minor center.

根据AFLP多态性数据绘制的聚类图,将139份木豆资源分为9个类群,并在分子水平上初步探讨了它们之间可能存在的演化关系,支持了木豆起源于印度,并在非洲东部形成次生起源中心的观点,并提出中国可能是栽培木豆的又一次生起源中心。

This study of polymorphism of human hereditary genes that associate with lung caner and alteration of related factor in lung cancer tissue will lay a foundation to reveal more secrets of lung cancer development.

我们研究人类先天遗传的、与肺癌相关的基因多态性及其相关因子在肺癌组织中的变化,将为揭开肺癌发生的奥秘提供有力依据。

RFLP and AFLP could be employed to make the linkage map and gene tagging by using the change of base pairs and the molecular rearrangement in restriction sites in specific restriction sites of DNA.

RFLP和AFLP技术利于花生图谱构建,利用DNA中特定的限制性酶切位点上碱基对的改变及酶切位点之间的分子重排,可以发现花生品种间的DNA许多多态性位点,进而绘制分子标记图谱。

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究,建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序;此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

Objective To investigate the lipoprotein concentration and apolipoprotein 〔apo〕 polymorphism in type 2 diabetic patients and its relationship to complications.

目的 研究2型糖尿病患者的脂蛋白〔Lp〕浓度和载脂蛋白〔apo〕的多态性以及与糖尿病慢性并发症的关系。

Objective: To investigate the association between the polymorphism of urotensin II gene and type 2 diabetes mellitus in Kun Ming.

目的:探讨尾加压素Ⅱ的基因多态性和昆明地区汉族人群的2型糖尿病患者胰岛素抵抗的关系。

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