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The 7 of 14 microsatellites had reached the homozygosis completely in familiesⅡat 18 The results suggested that the average N is decreasing and the genetic homozygosity is increasing with the improving inbred generation of WZSP.

WZSP近交系Ⅰ系F14-F16的14个微卫星座平均每个位点等位基因数为2个、平均杂合度为0.40、多态信息含量为0.26;Ⅱ系F15-F18的平均等位基因数为1.92,平均杂合度分别为0.32,多态信息含量为0.25;Ⅲ系F14-F17的平均等位基因数为2.17,平均杂合度为0.44,平均多态信息含量为0.32。

Meanwhile, Tibetan sheep (breeded in Sanjiaocheng of Qinghai, Huangcheng and Gannan of Gansu ), Mincounty Blank Fur sheep and Texel and Poll Dorset were controlled. The results indicated that polymorphism was tested at the loci of Hb, Tf and Es, that erythrocyte protein 1 and 2 were monomorphism. There were a couple of allele and three genotypes at the loci of Hb and Es. There were 10 genotypes at the locus of Tf, which was determined by 5 alleles.

结果表明,青海细毛羊和甘肃高山细毛羊的血红蛋白、转铁蛋白和酯酶基因座上存在丰富的多态性,其中Hb和Es位点受一对等位基因控制,共构成3种基因型;Tf位点受5个等位基因控制,共构成10种基因型;而红细胞蛋白质-1(EP-1)和红细胞蛋白质-2(EP-2)基因座均呈显单态;青海细毛羊的AMY基因座具有AMY1、AMY2、AMY3三种同工酶,其中AMY2呈多态,有AMY2A和AMY2O两种表现型,而甘肃高山细毛羊的AMY呈显单态。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析,分别提取19份马铃薯品种的DNA,进行随机引物多态性扩增,从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究,每条RAPD引物扩增出4~9条带,共获得52条带,其中多态性条带为43条;19份马铃薯品种的遗传距离介于0.17~0.72之间,平均值为0.39,平均遗传距离介于0.31~0.51之间;聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类,聚类结果与系谱分析基本相符,同时也说明克新系列马铃薯的遗传基础有所拓宽。

The biological polymorphism of Cercospora zeae-maydis was studied systematically in the levels of histology, protein and DNA. The major characters in polymorphism of pathogen were listed as follows:(1) The great differences were seen in shapes and sizes of conidiophores, the growth velocity of mycelium and pigment-productivity of colony among pathogen strains. In addition, an albino strain, a kind of mutant, was casually discovered during isolation of pathogen from diseased plants, which supported strongly our previous prediction that there should be some potential genetic variation in Cercospora zeae-maydis.

本研究在组织学、蛋白质和DNA水平上,系统研究了玉米灰斑病菌的生物学多态性,这种多态性主要表现在以下几个方面:①病菌形态学和培养性状多态性:菌株间存在分生孢子形态、菌丝生长速度、菌落产色素能力等方面的差异,该病菌在自然界存在另一种变异类型——白化菌株,说明灰斑病菌的确存在遗传变异的潜能。

The results showed that isozyme PAGE was rapid and inexpansive, but the munber of isozyme was lirnited. Endersperm of embryo protein PAGE was simple and a great deal of protein band could be found.

结果表明:同工酶电泳法速度快、成本低,而提供的多态性有限;胚乳或种胚蛋白质电泳法简单易行、在一些作物上具丰富的多态性;DNA电泳法能提供最丰富的多态性,但目前分析过程还较复杂。

Considering its complex geometric topology, multi states of self-equilibrium stress and modes of inextensional mechanisms, a prestress optimization strategy was proposed to solve the final feasible prestress distribution problem of the structures with multi states of self- equilibrium stress, where the decomposition and assembly method of modal matrix, the theory of structural equilibrium matrix and the singular value decomposition method were utilized.

考虑该结构几何拓扑复杂、多自应力模态与机构位移模态的特点,应用平衡矩阵理论与奇异值分解算法,通过模态矩阵的分解组合方法,给出一种具有一定普遍意义的预应力优化策略,使多自应力模态结构体系的最终可行预应力分布求解得以简便的实现。

Analyzing the DNA Polymorphism: 14 random primers were selected to analyze the DNA polymorphism of 13 species of Melampsora larici-populina, the result indicates: The isolates of MLP have resemblance and difference, thee differences reflect the DNA Polymorphism of them.But the Polymorphism is not related to their origin.the result is consistent with departed one.

2DNA多态性分析:用所选出的14条随机引物对13种落叶松-杨栅锈菌进行RAPD分析,发现落叶松—杨栅锈菌菌种之间既有一定相似性,又具有明显的遗传多态性且多态性与地理来源没有必然的相关性,这与以往的结论相一致。

No significant asˉsociation was difference of polymorphism and5-HTTSin2polymophism in different nation.Conclusion Our findˉings of the association study provided no evidence for an association between the5-HTTLPR,5-HTTStin2polymorˉphisms with anxiety-related personality traits in Han Chinese.

这两个多态位点在不同种族和人群中的分布有差异;相关分析结果不支持在中国对于5-HTTLPR、5-HTTStin2这两个多态位点在不同种族和人群中的分布有差异;相关分析结果不支持在中国汉族人群中这两个多态位点与焦虑相关人格特质有关。

Twelve microsatellite markers selected from the GENE-BANK were analyzed for polymorphism in silkies. Allele frequencies were estimated based on band presence or absence. Average heterozygosity and polymorphism information content were calculated by allele frequencies, and then, linkage analysis between character and markers by least squares means and the correlation among the 6 characters of egg traits in silkies were studied.

本试验选用12对微卫星引物,通过对丝羽乌骨鸡多态性扩增,计算出这些微卫星标记座位的等位基因频率、多态信息含量、群体杂合度;通过标记多态性与产蛋性状的最小二乘分析和多重比较,进行了分子标记与产蛋性状的相关分析,同时计算了产蛋性状间的表型相关。

On the basis of many times of experiments, A optimized reaction system about the microsatellite PCR amplification and partial COⅠ-Ⅱgene amplification of the green peach aphid was proposed. 3. It is clear that the result of polymorphic bands shows the abundance among the aphids after different UV radiation with three primers.

遗传多样性指标的方差分析结果表明,对于平均多态位点率,2 h处理是平均多态位点率的临界值,超过2 h, F2代平均多态位点率发生显著变异;对于平均基因多样度,对照与不同时间处理间差异显著;研究结果也表明低剂量紫外照射可增加蚜虫的免疫性,高剂量辐射则导致桃蚜的基因发生变异。

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