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The average interspecific divergence was 3.860%, and special sites were found in each chicken breed which was the bases to identifying chicken breeds. The DNA taxonomy of Bar1 used NJ was consistent with morphological taxonomy among the six indigenous chicken breeds. However, the analysis of Bar2 sequences of COⅠindicated that they were lower polymorphism and no special site in most chicken breeds which was not benefit to identify chicken breeds. The DNA taxonomy of Bar2 used NJ was not consistent with morphological taxonomy among the six indigenous chicken breeds.

本研究选择的2段序列中,以Bar1序列648bp(712位点到1359位点)多态性最高,突变位点最多,为24个,品种间平均多态性为3.860%,6个鸡种在此序列中有各自不同的特异位点,NJ聚类分析6个品种基本被聚为不同的类别,与形态学分类基本一致,该COⅠ基因的Bar1序列用于这些品种鉴定是可行的;而Bar2序列进行序列多态性分析,由于其多态性低,大多品种没有其特异位点,NJ聚类分析6个品种基本被聚为不同的类别,与形态学分类基本一致,不利于品种鉴定。

In my experimentⅠcompare the purposes of different combinations of endoenzyme(MseⅠ-HindⅢ, MseⅠ-PstⅠ, MseⅠ-EcoRⅠ) and find that MseⅠ-HindⅢeasily finds thepolymorphism by primers combinations suiTab for AFLP analysis.And by means of this there aremany polymorphism strips. So the double endoenzyme of AFLP was exercisedfor the analysis of soybean cytoplasmic male sterility.In the cource of my experiment, 64 primerpairs of MseⅠand HindⅢwere exerted to analyse the twe mtDNA pools which are made ofsterilities and holdings and the polymorphisms of sterilities and holdings.

运用扩增片段长度多态性的方法,对独特的杂交大豆试材进行分析研究,通过比较MseⅠ-HindⅢ、MseⅠ-PstⅠ、MseⅠ-EcoRⅠ不同酶切组合的效果,结果表明MseⅠ-HindⅢ较容易找到多态性引物组合,而且具有较高的多态性,本试验选用MseⅠ-HindⅢ进行双酶切大豆线粒体DNA从而完成AFLP的分析工作,试验用64对MseⅠ和HindⅢ引物对两个不育系及保持系材料的线粒体DNA制成的pool进行AFLP标记分析,分析了不育系与保持系之间的多态性; 4。

A total of 400 DNA fragments were amplified, among which 336 (84.0%) were polymorphic.

其中多态性带336条,平均多态位点水平为84.0%,表现出较高的多态性。

RAPD method was used to determine the molecular nature of the variants, and polymorphism, such as OPD05-1250 and OPH16-800 in NaCl-tolerant line of sainfoin and OPA08-1180 and OPH01-760 in ethionine-resistant cell line alfalfa, could be clearly detected among different lines, indicating these variations were caused by the changes in DNA.

用RAPD技术对红豆草和苜蓿几种变异系的DNA多态性进行比较分析,证实红豆草耐盐系中具有OPD05-1250、OPH16-800等多态性产物,苜蓿变异系中具有OPH01-760、OPA08-1180多态性产物。说明变异系中DNA水平发生一定改变。

Fourteen wampee cultivars collected from Guangdong and Guangxi were used for RAPD analysis in this study. 16 primers selected from 150 random primers were used to amplify 87 DNA fragments and 57 bands (54.0%) showed polymorphisms.

摘 要:对14个黄皮品种进行 RAPD 分析,从150个随机引物中筛选出16个能在种质间表现出多态性的引物,共扩增出 87 条谱带,其中多态性带47条,多态性比例为54.0%。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应-单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26, Exon 30)及21内含子(Intron21)基因多态性,进行基因序分析,研究这些基因多态性是否存在连锁关系;应用Fok1酶切法对147例血液病人人类血小板抗原-3(human platelet antigen-3, HPA-3)基因进行分型,并与110例正常人进行比较;将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组,血小板输注3次以后检则血小板抗体。

Methods The polymerase chain reaction-restriction fragment length polymorphism was used to analyze the gene polymorphism of ApoE in 25 high carotid artery IMT patients, 25 carotid artery sclerostenosis patients and 25 healthy age-matched controls.

应用聚合酶链反应-限制性片段长度多态性技术,对25例B超检测颈动脉内中膜增厚患者和25例颈动脉粥样硬化性狭窄患者及25例同期健康体检者的ApoE基因多态性进行分析,观察其多态性。

In this study, We typed the the gene of β〓-AR from genomic DNA of asthmatic patients and made the further analysis: The distributing condition of genetic polymorphisms at positions 16 and 27 in β〓 AR coding region and at position 19 in 5'Leader Cistron (5'LC) among southwest Chinese asthmatic population; The relationship between the each polymorphisms at the above three positions and the asthmatic phenotypes (FEV〓 or nocturnal asthma); The effect of each polymorphisms at the above three positions on bronchodilator responsiveness induced by β〓- agonists(initial bronchodilator response, pharmaco-dynamic); The relationship between the haplotypes of polymorphisms in the 5'LC or in positions 16, 27 of β〓AR coding block and asthmatic phenotypes or bronchodilator responsiveness induced by β〓-agonists.

实验通过对哮喘人群的β〓-AR基因进行分型,探讨:β〓-AR编码区16、27位点及5'前导肽(5'LeaderCistron,5'LC)19位点遗传多态性在中国西南地区哮喘人群中的分布情况;三个位点多态性与哮喘临床表现型的关系;三个位点多态性对β〓受体激动剂反应性(支气管舒张反应,舒张反应动力学)的影响;16、27位点及5'前导肽19位点遗传多态性单倍型与哮喘临床表现型及β〓受体激动剂反应性的关系。

Our article mainly discussed the preparation of entangled states and the application of entangled states in quantum communication, including practical scheme for remote state preparation of a photon-photon entangled state, generation and concentration of multi-atom entangled states, practical protocol for telecloning quantum states and protocol for multi-party superdense coding by using multi-atom in cavity QED.This article has several objectives.

本文主要研究了量子纠缠态的制备及纠缠态在量子通信中的应用,包括远程制备光子-光子纠缠态,产生和浓缩多原子纠缠态,利用纠缠态进行远程克隆以及通过腔QED实现多组份密集编码的方案。

ALb and Ams of Angora and Tibetan foat had only one phenotype,they did not present polymorphism.The Tf of Angora goat had not polymorphism,but that of Tibetan goat had its polymorphism,The Akp,LDH and Es in goats of both breeds had polymorphisms,eapecially the phenotype of Es was more complex.

安哥拉山羊和藏山羊 Hb、Alb 和 Amy 均只有一种表型,未表现出多态性,安哥拉山羊 Tf 也无多态性,藏山羊则有多态性;而两个品种山羊的 AKP、LDH 和 Es 均表现出多态性,尤其是 Es 的表型更为复杂。

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