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Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上,根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点,设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针,优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件,建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法,组装了牛、羊、猪种布鲁氏菌检测试剂盒,该方法能特异检测上述3个种的布鲁氏菌。

Consequently, genetic polymorphisms in both nicotine metabolism and dopamine catabolism genes may influence smoking behavior, and interact with each other resulting in risk modulation.

因此,在尼古丁代谢和多巴胺分解中的基因多态性可能会影响吸烟行为,它们的相互作用导致风险的调节。

The prolactin gene was studied as a candidate gene for the prolificacy of five different sheep breeds, namely small tail Han sheep (142), Hu sheep (40), Texel sheep (36), Dorset sheep (36) and Corriedale sheep (28). Single nucleotide polymorphisms of PRL coding sequence were detected among these five sheep breeds.

本研究以小尾寒羊(142只)、湖羊(40只)、特克塞尔羊(36只)、多赛特羊(36只)和考力代羊(28只)这五个绵羊品种为实验材料,应用PCR-SSCP对PRL基因的DNA编码序列的多态性进行了分析,并进一步对该基因与小尾寒羊高繁殖力之间的关系进行了研究。

Results The rs1549637 of PLA2G4C gene was not associated with the illness (P>0.05), but the genotypes were associated with the symptoms of schizophrenia, i.e. Delusion of reference, Delusion of grandeur, Hallucination-delusion.

结果 PLA2G4C基因上的 rs1549637位点基因多态性与精神分裂症无统计学关联(P>0.05),但该位点的基因型频率与精神分裂症的关系妄想、夸大妄想和幻觉-妄想综合症等多个临床症状相关联(P<0.05)。

Most of the ASIC's ever designed are driven by multiple asynchronous clocks. An important problem in multi-clock domain design is how to avoid metastability.

摘 要:绝大部分ASIC设计工程师在实际工作中都会遇到多时钟域设计的问题,多时钟域设计的一个难题是如何避免亚稳态的产生。

Methods:The environmental factors of 80 CHD children and their parents were analysed by a case-control study,and the polymorphism of MTHFR gene 677C situs and plasma Hcy level were detected,logistic regression analysis was carried out in the study.

采用病例对照研究方法,分析80对CHD患儿与对照儿父母所处的环境因素并检测其MTHFR677位点的多态性和血浆Hcy的水平,进行单因素及多因素Logistic回归分析。

More and more results indicate, following the plan of human genome to accomplish, that there has close relation between the susceptivity of desease and the polymorphisms of human gene.

随着人类基因组计划研究的完成,越来越多的研究证实,人类基因多态性与疾病易感性之间有着密切的关系。

Xylan in nature is a complex of many polysaccharides, and this makes it appears polymorphism and poly-sphere, therefore, it is necessary to classify xylanase of great variety.

自然界中木聚糖是多种糖类的复合体,这就使得木聚糖酶呈现多态性和多域性,由此需将繁多的木聚糖酶进行归类。

Methods Polymerase chain reaction was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene.

应用多聚酶链反应技术扩增HPV16 DNA和Ha-ras癌基因相关片段,分别采用2%琼脂糖凝胶电泳和限制性片段长度多态性分析,检测口腔癌中的HPV16 DNA和Ha-ras癌基因第12位密码子的点突变。

The point mutations of the 12th coden of ras gene and p53 E5 and E7 gene were studied in 40 cases w ith laryngeal squamous cell carcinomas by PCR-SSCP(polymerase chain reaction-s ingle stranded conformational polymorphism).

应用多聚酶链反应-单链构像多态性分析方法分析40例喉鳞状细胞癌H-ras,K-ras,和N-ras基因的第12密码子,p53 基因第5,7外显子中点突变的情况。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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