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Pro-opiomelanocortin plays an important role on animal ingestive behavior and energy homeostasis. Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds (i.e., Qinchuan, Nanyang, Jiaxian Red cattle, Jinnan, Luxi, Angus, and Chinese Holstein), and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Nanyang cattle.

为研究阿片黑皮质素前体在动物采食和能量平衡调控中发挥重要作用,文章采用PCR-SSCP结合DNA测序方法,对秦川牛、南阳牛、郏县红牛、晋南牛、鲁西牛、安格斯牛和荷斯坦奶牛共计480头个体POMC基因的多态性进行研究,并分析了多态位点与南阳牛生长性状的相关性。

Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls, and 120 cases diagnosed as CHD. The PPARδ+294T/C gene polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphisms. The frequencies of PPARδ+294T/C genotypes and the "C"allele frequency were analyzed, to evaluate the risk for the CHD among variant genotypes.

运用聚合酶链式反应及限制酶片段长度多态性技术分析无血缘关系汉族人群[包括82例正常对照者,120 例冠心病患者]的PPARδ+294T/C基因多态性,分析基因型频率、等位基因频率,并对不同基因型患者冠心病的危险性进行评价。

Acerifolia. Here, 16 primers out of 38 ISSR primers screened, were found to produce clear reproducible bands resulting in a total of 103 distinct bands, Of these 103 bands, 86 were monomorphic across all 20 of the plants tested and 17 showed polymorphisms (16.5% polymorphism).

研究结果表明:从38条ISSR引物中筛选得到16条引物可以产生明亮清晰、重复性高的条带,条带总数为103条,其中86条条带表现为一致性,在20个快繁苗中无任何差异;而剩下的17条条带表现为多态性(多态率16.5%)。

The genetic relationships of 11 cultivars and lines in Pennisetum were analyzed using random amplified polymorphic DNA markers. A total of 82 reproducible RAPD fragments were produced using 8 random primers.

利用RAPD分子标记技术分析狼尾草属11个品种12份材料间的遗传关系,结果发现7个引物共扩增出82条带,其中多态性条带为60条,多态位点达73.2%,表明在这11个品种间具有较高的遗传多样性。

Objective: To study the distribution of genetic polymorphism of VWA, FGA, PENTA E loci in Mongolia population and construct genetic database.

目的 :了解内蒙古锡盟地区蒙古族人群 VWA、FGA、PENTA E三个 STR位点的遗传多态性分布,获得相应多态位点的群体遗传学数据。

Results (1) From more than 100 primers, 11 primers producing polymorphism and reproduceable bands were selected, 129 bands were amplified.(2) The percentage of polymorphic bands within different populations were 20.9%-55.0%.(3) The cluster map including all samples were obtained by UPGMA. In the map, there were six cluster groups and three individuals outside the groups. The branch with five samples of Zhongjiang Sichuan population were far from other samples in genetic distance.(4) According to the distribution provinces five groups were divided in genetic variance analysis. Genetic variance 80.44% existing within population, 8.29% among populations and 11.27% among groups.

结果 (1)从100多个引物中选择出11个多态性强和重复性好的引物,扩增得到129条带;(2)不同居群内多态位点比率为20.9%-55.0%;(3)用UPGMA法得到所有样本的聚类图,可分为6个主要分支组和3个组外个体,其中四川中江居群的5个样本聚为一组,并与其他样本遗传距离较大;(4)按产地分组,遗传差异的80.44%存在于居群内,8.29%来自于组内居群间,11.27%的遗传差异来自于组间。

Investigations focused on 3 polymorphisms in retinoid X receptors RXRA and RXRB gene associations.

研究集中在维甲类X受体RXRA和RXRB两个基因的3个多态性上,研究了这些多态性与银屑病的关系。

Amplified fragment length polymorphism was used to analyze the genetic diversity of 92 Torreya grandis from 6 different populations. Total of 364 loci of the T.grandis genome were examined for molecular variation and 63 loci were polymorphic.

利用AFLP分子标记技术,采用8对引物对6个香榧天然群体的92个个体进行了总基因组DNA水平上的多态性检测,共检测出364个位点,其中多态性位点63个。

And 4. 39% respectively, While the results were 94. 44%, 2. 78% and 2. 78% respectively in unrelative Chinese Uigurs, furthermore, all 6 cases of partial minorities were RHD〓/RHD〓 types.

启动子区1000bp范围内4个RHD基因的多态性位点检测结果显示,RhD阴性样本中,存在RHD基因序列的样本均可检测到这4个多态性位点。

Objective To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease.

目的利用基因芯片检测乙醇代谢相关酶的单核苷酸多态性,并阐明乙醇代谢相关酶基因多态性与酒精性肝病的关系。

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