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STR locidistribute extensively and have higher information, polymorphism, and its heredity is based on Mondel codominant inheritance. We usehalf-quantitative analysis of STR polymorphism loci, to set up a clinicalpractical method that is highly accurate in rapidly detecting and prenatalgene diagnosing Down syndrome.

STR广泛分布于人类基因组中,一般认为,人类基因组平均每6~10Kb就有一个STR基因座,STR有高度多态性并遵循孟德尔共显性遗传,具有操作简便、分型准确和丰富的多态信息量等特点,已成为第二代DNA分析技术的核心。

The gonadotropin releasing hormone receptor gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 1, exon 2 and exon 3 of GnRHR gene in both high fecundity breeds and low fecundity breeds (South African Mutton Merino, Corriedale and Chinese Merino sheep) by PCR-SSCP. Only the products amplified by primers P4 and P7 displayed polymorphisms.

设计8对引物,应用聚合酶链式反应-单链构象多态性技术检测促性腺激素释放激素受体(gonadotropin releasing hormone receptor, GnRHR)基因外显子1、外显子2和外显子3在高繁殖力品种和低繁殖力品种(南非肉用美利奴、考力代和中国美利奴绵羊)中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。

These data indicate that it is feasible to establish universal primers and transferable EST-SSR markers between monocotyledonous and dicotyledonous crops.

22对白菜/油菜EST-SSR引物中,有18,21和22对引物分别在大豆、小麦和玉米中有扩增产物,可扩增率分别为81.8%,95.4%和100%;其中分别有10对和7对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性,有7对引物在4种作物中的扩增产物均显示多态性。

Twenty-two of these 143 polymorphic bands were found non-special, accounting for 15.4% of the total. It is assumed that they was caused by difference of the conservative sequence.

在上述143条多态性条带中,22条为非特异性条带,占多态性条带总数的15%,它可能与这2个香蕉野生近缘种中差异的保守序列有关。

Then further analysis of selected esterase was carried out on polyacrylamide gel electrophoresis.

EST的PAGE酶谱显示出较高的多态性,各品种之间差异显著,多态性酶带占80%。

Seen from the statistical comparision,the ABS and genetical distance of subgroups were 99.2%,0.008 respectively.

统计比较,公羊亚群的多态位点占51%,母羊亚群的多态位点占48.4%。

The single nucleotide polymorphism of growth hormone gene was investigated in various breeds of duck, including Beijing ducks, Xihu mallards, Jinding ducks, Shan Partridge ducks, Jingjiang ducks and Shaoxing ducks. The primers for intron 2 and 3 in GH gene were designed based on the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method.

根据鸭生长激素基因内含子2、3的序列设计5对引物,利用PCR-SSCP方法对北京鸭、西湖野鸭、金定鸭、山麻鸭、荆江鸭、绍兴鸭等6个鸭品种进行了单核苷酸多态性分析,并检测其多态性。

RAPD(Random amplified polymorphic DNA) was used to detect the genetic diversity of Castanopsis eyrei populations in three forest communities at different succession stages in Tiantai mountain Zhejiang Province,namely,needle-leaved forest,needle/broad-leaved mixed forest and evergreen broad-leaved forest.

利用RAPD技术对甜槠种群在浙江省天台山不同演替阶段森林群落(针叶林、针阔混交林、常绿阔叶林)中的遗传多样性和遗传分化进行了分析。3个甜槠种群平均多态位点百分率为67.98%,总多态位点百分率为92.13%。

The single-strand conformation polymorphism method was used to analyze the polymorphism in 3'-flanking region of the HSP70 gene.

同时设计引物扩增 HSP70基因3'-侧翼区,通过PCR-SSCP技术分析 HSP70基因3'-侧翼区的多态性,并发现一个多态位点。

In this thesis three kinds of piezoelectric transformers are investigated, including circular cylinder piezoelectric transformer of thickness-shear mode, cylindrical thin shell piezoelectric transformer of torsional mode and multi-layered rectangular plate piezoelectric transformer of thickness-twist mode.

本文研究了三种不同结构模态的压电变压器,包括基于厚度剪切模态的圆筒结构压电变压器、基于扭转模态的壳结构压电变压器和基于厚度扭转模态的多层矩形板结构压电变压器。

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