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Analyzing T-DNA flanking sequences isolated by TAIL-PCR revealed that the T-DNA nick position in the tagged rice plants are more complex than in tansgenic dicot species as tobacco and Arabidopsis.

分析TAIL-PCR分离的T-DNA侧翼序列,发现水稻转化体中T-DNA右边界的剪切位点比转基因拟南芥和烟草等双子叶植物中T-DNA右边界的剪切位点要复杂得多。

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目:国家生命科学与技术人才培养基地科技创新基金项目(2007088);国家油菜产业化项目科学家岗位"化杀岗位"(nycytx-00505);国家自然科学基金项目(30871578)中文摘要:探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物,分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板,进行PCR扩增及扩增产物的多态性分析。

Using nuclear DNA C-values for 539 angiosperms in China, we examined the variation of these values among growth forms and taxonomic groups and the relationship of these values with invasiveness. Mean DNA C-value of the 539 angiosperm species was 4.06 pg. Mean DNA C-value was(1) significantly lower for woody species (1.84 pg) than for herbaceous species(5.02 pg);(2) significantly lower for 360 dicots (2.20 pg) than for 179 monocots (7.80 pg);(3) significantly lower for annuals (2.78 pg) than for perennials(6.65 pg);(4) significantly lower for 134 weed species (1.93 pg) than for herbaceous non-weeds (6.75 pg) and for several families that have an unusually high proportion of weed species;(5) significantly lower for 47 exotic weed species (1.76 pg) than for 134 native weeds (1.93 pg), but significantly lower than that of "non-weedy" herbaceous species (6.75 pg);(6) lower for weeds than for "non-weedy" species in same genus or family; and (7) in herbaceous species, generally lower for weedy compared to "non-weedy" species, with some exceptions such as Avena fatua, whose DNA C-value is as high as 14.15 pg, contrarily, and some "non-weedy" herbaceous species in Cruciferae and Cucurbitaceae with very low values.

统计了中国境内有分布的539种被子植物的DNA C-值,分析了它们在不同分类群、生活型、倍性、生活史类型以及在杂草和非杂草类群中的分布情况,主要结果如下:(1)539种被子植物DNA C-值平均为4.06 pg,其中木本植物的DNA C-值平均为1.84 pg,低于草本植物的平均值(5.02 pg);(2)双子叶植物(360种)的DNA C-值平均为2.20 pg,极明显地小于单子叶植物(179种)的平均值(7.80 pg);(3)1年生植物的DNA C-值平均为2.78 pg,明显小于多年植物的平均DNA C-值(6.65 pg);(4)134种杂草的DNA C-值平均为1.93 pg,明显小于非杂草草本植物的平均值(6.75 pg),含杂草较多的科,平均DNA C-值相对较小;(5)统计的47种入侵杂草的DNA C-值平均为1.76 pg,略小于134种杂草的平均DNA C-值(1.93 pg),极显著地小于非杂草性草本植物(6.75 pg);(6)以科为单位,不同科的DNA C-值存在着极大的差异;(7)DNA C-值与染色体倍性的关系并不明显,但是,随着倍性的增加,基因组变小;(8)在同一科、属中,与非杂草相比,典型杂草的DNA C-值往往偏小;(9)总体上杂草或杂草性强的植物,它们的DNA C-值比非杂草性植物的要小。

The embryogenic cell first divided into multicellular proembryos,then globular embryoid,pearshaped embryoid,long embryoid,scutiform embryoid and mature embryoid.

胚性细胞继续分裂形成多细胞原胚,经球形胚、梨形胚、长形胚、子叶分化期到成熟胚,其发生的胚状体与单子叶合子胚形成具有相似的过程。

Pome drupe-like, purplish black or bluish, subglobose, succulent, with early caducous sepals leaving an annular ring; seeds 1 or 2, subglobose, large, seed coat thin, cotyledons thickened, plano-convex or semiglobose.

象Pome核果一样,略带紫色黑色或者带蓝色,近球形,多汁,带有早落萼片留下一个环形的圈;种子1或2,近球形,大,瘦的种皮,子叶加厚,平凸的或半球形。

During late winter the leaf color is best described as dark green with a bronze hue.

黄杨是双子叶植物纲蔷薇亚纲中的一科,有约100种之多。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。