多壳的

This paper deals with the partial capacitance calculation of the high voltage transmission line for the neutral beam injectors.

该文研究了中性束注入系统中高压传输线部分电容的求解问题；根据传输线多层同轴导电壳的结构特点，利用多重镜像法确定与平行导线相应的一系列的镜像线电荷，求解的问题变为由一系列平行线电荷构成的电场问题，传输线的部分电容则通过各导体间的电位系数来求得；这一方法避免了直接求解的困难以及大量数值计算等问题。

All results above account for that CO_2 gas in Jiyang Sag have multi origin and mainly abiogenetic, and mantle is the dominating source, a few may come from biogenesis; and in the process of gas pools forming, some mantle CO_2 lost by converted to CH4 or carbonate, some crust CO_2 added into the gas pools; CO_2 gas pools in Huagou district have particular geochemical character, maybe there were some decarbonated CO_2 added into the gas pools, need further research work.

以上种种特征都说明，济阳坳陷非烃类气藏中气体具有多来源的特征，但CO_2主体是无机成因，并主要来自地幔，少量来自有机成因；在成藏过程，幔源CO_2通过转变为CH_4或者碳酸盐岩而丢失了部分CO_2，壳源CO_2和其它烃类加入到气藏中；花沟地区的气体地球化学特征与众不同，可能存在碳酸盐地层变质脱气产生的CO_2的混入，但具体原因尚待进一步研究。

If your demands are higher, you can use the Silver Key Shell to create more advanced parcels.

如果您的要求较高，您可以使用银的关键壳，以创造更多先进的包裹。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

MAESTRO can be used to analyze and design many types of thin-wall stiffened structures, and has been used for virtually every type of ship from large tankers, container ships and bulk carriers to high speed ferries, multi-hull vessels, and SWATHs, as well as smaller vessels such as fishing vessels and patrol craft.

MAESTRO可以用来分析和设计的多种船筋结构可应用于几乎所有类型船舶的大型油轮，集装箱船和散装船的高速船，多壳船等的设计，以及小型船只，如渔船和巡逻艇。

Related to sea slugs, nudibranchs are shell-less mollusks that display some of the most vibrant colors found anywhere in nature.

有关联的是海蜗牛，nudibranchs是很少壳的软体动物显示其最多的充满活力的色彩在各处的自然界。

First acts according to the igniter plug iron casing the product detail drawing, the production scale and the concrete working condition, had determined the design craft principle, formulates some feasible craft plans, and has carried on the analysis to each kind of plan, finally selected set of best reasonable crafts plans - to use the bean material: The multi- locations cold extrusion takes shape.

首先根据火花塞铁壳的产品零件图，生产规模及具体生产条件，确定了设计的工艺原则，制定一些可行的工艺方案，并对各种方案进行了分析，最终选出了一套最佳合理的工艺方案——豆料：多工位冷挤压成形。

First acts according to the igniter plug iron casing the product detail drawing the production scale and the concrete working condition had determined the design craft principle formulates some feasible craft plans and has carried on the analysis to each kind of plan finally selected set of best reasonable crafts plans - to use the bean material: The multi- locations cold extrusion takes shape.

首先根据火花塞铁壳的产品零件图，生产规模及详细生产条件，确定了设计的工艺原则，制定一些可行的工艺方案，并对各种方案进行了分析，最终选出了一套最佳合理的工艺方案——豆料：多工位冷挤压成形。

Shallow-water echinoderms having soft bodies enclosed in thin spiny globular shells.

浅水中的棘皮动物，其软的身体封在多刺的球形壳中。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了