多型性

This study attempts to use the statistical techniques of multilevel confirmatory factor analysis to model the measurement of learning school. The analyses follow the four initial steps proposed by Muthèn: conventional CFA of total structure, estimation of between-group variation, estimation of within-group structure, and estimation of between-group structure.

本研究采用多层次验证性因素分析的统计技术来模式化学习型学校的量表，分析依循Muthèn(1994)所提出的四个先行检定步骤：对整体结构做传统的验证性因素分析、组问变异的估计、组内结构的估计，以及组间结构的估计。

Under the conditions of Ambrosetti-Rabinowitz type superlinear and odd duality, we show that the systems have at least one nontrivial solution and infinitely many solutions.

在Ambrosetti-Rabinowitz型超线性条件及具备奇对偶性条件下，得到了方程组分别有一个解及无穷多个解的存在性结果。

As an ideal prime immunization vaccine,the DNA vaccine has the advantages of high purity,continuous expression of antigens with low dose, repeatable immunization and inducement of memory CTL with high titer.Similarly, recombinant virus can not only highly express the target antigen,simulate the inducement process of virus infection on body immunity,and induce various cytokines,but also be beneficial to the interaction of high-affinity antibodies with T cell receptors,and the generation of Th1 reaction and CTL reaction.

DNA疫苗抗原纯度高，持续表达低剂量的抗原，可多次重复免疫以及诱导高效价记忆性细胞毒性T淋巴细胞反应，是理想的用于初次免疫的疫苗；重组病毒能高效表达目的抗原，模拟病毒感染诱导机体免疫的天然过程，能诱发多种细胞因子，而且有利于高亲和性抗体和T细胞受体的选择，可以有效地产生Th1型和CTL反应。

Started from D-tyrosine, we obtained the tetramic acid 96 using Jouin reaction. The assembly of the second fragment 109 was stared from 1,4-butandiol,using asymmetric alkylation reaction developed by Oppolzer as a key step. Coupling of the tetramic acid 96 with 109 provided the key intermediate 110 according to Yoshii\'s procedure. After remove protective group and Sharpless epoxidation, we obtained the key intermediate 113. Its macrocyclization to form the final product is in progress.

本论文的第二章主要对Macrocidin A开展了合成工作，我们从D构型的酪氨酸出发，经过官能团转换；利用Jouin反应生成Tetramic acid 96，另外一个片断从1，4-丁二醇开始经过多步反应生成碘化物104，利用Oppolzer发展的手性樟脑磺酰胺为辅基参与的不对称烷基化反应，引入手性甲基，生成关键中间体109，接着通过Yoshii发展的方法连接96和109生成110，经过Sharpless不对称环氧化反应建立环氧化合物，然后脱去保护基得到关环前体113，分子内的Mitsunobu反应进行关大环反应正在进行之中。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

There are several characters in our researches:(1) we study the relationship between karyotype polymorphism and sex chromosome in Elaphodus Cephalophus which only lives in China, and explore the mechanism of its sex chromosome evolution.

研究特色主要体现在：一、以我国特有的动物毛冠鹿为材料，在染色体和DNA水平上研究毛冠鹿核型多态与性染色体关系，探索毛冠鹿性染色体发生和演变的机制。

In the favorable inoculation season, the induction percentages of meristematic globules from flowers and embryogenic calli from scalps were 15～80％ and 0～10％ respectively, depending on the cultivars and incubation conditions.

从多芽体获得胚性愈伤组织的概率大约为0～10％，胚性愈伤组织的诱导率受基因型和品种、接种时期及培养条件等因素的影响。

Cerevisiae have found that bZIP proteins play a role in yeast anti-oxidation. at3g51960, the transcription factor found in plants, is highly homologous to that of yeast. To examine the in vivo role of at3g51960, the overexpression and silencing vectors were constructed respectively. By using flower dip-dye, the binary expression vectors, pCAMBTA3301: at3g51960 and pCAMBTA3301: at3g51960Promoter, as well as vectors pCAMBTA3301: at3g51960-GFP and pCAMBTA3301: at3g51960cDNA were transformed to Arabidopsis by Tumefaciens. The transgenetic homozygous Arabidopsis strains have been derived after screening. Analysis on these strains reveals that strains overexpressing at3g51960 gene have higher level of stress resistance, implying a role of at3g51960 in plant stress regulation.

酵母中发现了bZIP家族的转录因子与酵母的抗氧化性相关，植物中的转录因子at3g51960与酵母中的这一转录因子在空间结构上有很高的同源性，为了验证它在体内的功能，本实验构建了过量表达载体和沉默载体，利用花浸染法把农杆菌携带的双元表达载体，pCAMBTA3301： at3g51960，pCAMBTA3301： at3g51960 Promoter和pCAMBTA3301： at3g51960-GFP及pCAMBTA3301： at3g51960 cDNA转入拟南芥，通过多次筛选已经获得了转基因拟南芥的纯合植株，对获得的转基因植株的表型分析发现，在过量表达at3g51960的转基因拟南芥中对胁迫的抗性有一定程度的变化，因此推测at3g51960可能参与植物的抗性调节。

Behavior traits such as affability and obedience to human, fetch and retrieve, tug of war, fear and courage were tested in 204 two-month old puppies from 3 breeds (German Shepherd Dog, Labrador Retriever, English Springer Spaniel).

测定了204条德国牧羊犬、拉布拉多犬、史宾格犬二月龄幼犬对人依恋性和服从性、衔取、争夺游戏和胆量等行为性状，同时用PCR-RYLP方法检测各犬单胺氧化酶B基因型。

Seventeen genotypes of soft wheat were sown in three locations for two years to study the genetic variation of the water-soluble pentosans, water-insoluble pentosans, and total pentosans, to investigate the effects of genotype, environment and their interaction on pentosans, and to determine the relationships between pentosans and other quality parameters.

将17份软质小麦品种进行了两年多点试验，分析了水溶性戊聚糖、非水溶性戊聚糖和总戊聚糖含量的遗传变异和基因型、环境及其互作对其影响；同时研究了3种戊聚糖含量与其它品质性状的关系。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。