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Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型,通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用;采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用;利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

NaCl and iso-osmotic PEG had inhibitory effect on the germination of two halophytic seeds, and the inhibitory level of PEG was greater than that of iso-osmotic NaCl. Inhibitory effects of NaCl and water stress mainly showed lowering the seed germination rate, delaying initial time of seed germination and extending the time of seed germination.

本研究从生理和分子角度对其耐盐性进行了初步的探讨,开展这两种盐生植物耐盐相关基因的克隆及盐爪爪液泡膜上多个耐盐相关基因的遗传工程,为作物和林木的遗传改良奠定坚实的理论和实验基础。

The organic/inorganic composites, as new materials that combine the inorganic phase"s rigidity,stability and the organic phase"s toughness and workability, have important theoretical and realisticsignificance in materials science. C_(60) is a brand-new holosymmetry spherical molecule with theunique physical and chemical property, displaying wide implications and applications of physics,chemistry, biology, materials science, medicine, electronics, and so on.

有机/无机复合材料集无机相的刚性、稳定性和有机相的韧性、加工性等诸多特性于一体的一类新型材料,具有重要的理论和现实意义。C_(60)是一个全新的全对称球形分子,具有独特的物理、化学性能,已广泛地影响和应用到物理、化学、生物学、材料学、医学、电子学等许多领域。

That experiment and subsequent ones showed that simple chemistry can transform nonorganic molecules into the building blocks of life.

然后用人造闪电去打击它。这个实验和往后追加的更多的实验证明了简单的化学反应就能把无机的分子转变为生命的基本建材。

Recent developments of clean oxidation systems catalyzed by polyoxometalates are summarized in detail. The emphasis is on the epoxidation reaction of olefinic compounds, oxidation of alkanes and aromatic compounds with the comparatively ideal oxidants, hydrogen peroxide and molecular oxygen respectively.

文本总结了多金属氧酸盐在液相清洁催化氧化方面的进展,主要针对以过氧化氢和分子氧为氧化剂的烯烃类化合物的环氧化反应、烷烃和芳香族化合物的氧化反应,并详细探讨了此类氧化反应的反应机理。

Recent developments of clean oxidation systems catalyzed by polyoxometalates are summarized in detail. The emphasis is on the epoxidation reaction of olefinic compounds, oxidation of alkanes and aromatic compounds with the comparatively ideal oxidants, hydrogen peroxide and molecular oxygen respectively. The oxidation mechanisms are elucidated in detail.

文本总结了多金属氧酸盐在液相清洁催化氧化方面的进展,主要针对以过氧化氢和分子氧为氧化剂的烯烃类化合物的环氧化反应、烷烃和芳香族化合物的氧化反应,并详细探讨了此类氧化反应的反应机理。

The CMS line 9704A, the restorer 8001 and F, population of 9704AX8001 containing 120 individual plants were used for molecular mapping of the restorer gene by bulked segregant analysis. 515 decamer primers with arbitrary sequences were chosen for polymerase chain reaction amplification. It is found that the polymorphism between the parents could be detected by 43. 8% of the primer and one RAPD marker OPL09-76I was tightly linked to the restorer gene of the restorer 8001 with the overcross value of 4.17% and genetic distance of 4. 18cM. The marker was repeatable and easy to score.

以辣椒CHS系9704A、恢复系8001及其组合9704A×8001F_2群体120株植株为材料,利用群分法法进行恢复基因定位,通过515个随机引物的PCR扩增分析,发现43.8%的引物在双亲间表现出多态性,找到一个与辣椒CMS恢复系8001中的恢复基因紧密连锁的分子标记OPL09_(-763),该标记与8001的恢复基因的交换值为4.17%,遗传距离为4.18cM。

The birds of Passeriformes are the group which are the largest number, the most species, the highest station of evolvement and the broadest adaptive radiation of existent aves. The traditional taxonomy were onlyaccording to morphologic and behavioral characters, however their morphologic and behavioral characters are so complicated that we could not differentiate some species easily. Therefore we had some taxonomic research by molecular means in order to supply traditional taxonomy.

雀形目是现存鸟类中种类最多、数量最大的、演化地位最高、适应辐射最广的类群,传统的分类方法仅依据形态和行为特征来划分,但由于该类群的形态及行为特征复杂使得部分种类不易区分,因此有必要依据分子手段研究其系统发生关系、以盼能对经典的分类方法给予补充。

Predicted Model of Aggregation of Molecules in Chinese Herbal Drugs by Support Vector Machines;2. The aggregation behavior of phenylhydrazone and hemicyanine,in Z-type LB multilayers has been studied by absorption, steady state and time-resolved fluorescence spectroscopies.

利用吸收、稳态和时间分辨荧光方法研究了苯腙和半花菁分子在Z型LB多层膜中的聚集体性质,苯腙和半花菁吸收光谱的红移和兰移,说明它们分别以J和H型聚集体形式存在于LB腹中苯腙和半花菁LB膜的荧光发射带分别在560nm和584nm处,亦分别对于它们溶液的荧光谱显示出红移和兰移的特征。

In order to further enrich and extend the studies on functional complexes, the molecular magnetism, kinetics of catalytic hydrolysis and biological activities of bridged polynuclear complexes have been studied systematically in this dissertation.

本论文围绕桥联多核配合物的分子磁学、催化水解动力学及生物活性等前沿课题进行了较深入和系统的研究,为进一步丰富和拓宽功能配合物的研究提供了一定的理论依据和某些有用的信息。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。