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The synthesis of the pentacyclic skeleton of Et-743 and the construction of the five chiral centers thereof.1. The core pentacyclic skeleton of phthalascidin was constructed with L-dopa as the starting material via thirteen steps. First, two basic synthons, the C-1 functionalized tetrahydroisoquinoline compounds 8 and N-protected Z-dopa derivative 9, were prepared from L-dopa. They were coupled via amide bond to form the dipeptide compounds 10, which were the first key intermediates. Then the primary hydroxyl groups were transformed into the corresponding amino aldehydes 11 via Swern oxidation. Followed by the intramolecular Pictet-Spengler cyclization, the pentacyclic compounds 12 were synthesized.

五环骨架的构建及五个正确手性中心的确立:1.1我们以L-多巴为起始原料,经过多步官能团保护和转化合成了基本结构单元8然后和苯丙氨酸衍生物9,通过缩合反应生成酰胺中间体10,经Swern氧化得到醛化合物11,然后再次利用分子内改良的Pictet-Spengler反应一步构建c,d两环,得到五环骨架化合物12,最后,实现了21-位羰基向氰基的转化,得到了C-1位官能化的化合物14,化合物14不但具有和Et-743一致的五环骨架,而且分子中五个手性中心立体构型也与其完全相同:其中,3,位和13,位的手性中心由原料L-多巴带入,而1,位,11,位和21,位手性中心则通过不对称诱导而确立。

Reporter molecules (thiophenol, bipyridine derivates, cyanopyridine derivates) were attached to different immuno-gold colloids, respectively. By means of the specific adsorption between antigen and antibody, a sandwich structure was constructed, in which labelled immuno-gold colloids and antigen covalently bound to substrate specifically capture different antibodies.

以多种不同的标记分子(苯硫酚,联吡啶类分子,氰基吡啶类分子)分别标记多种不同免疫金溶胶,通过抗体抗原之间所具有的特异吸附性,进一步组装"固相抗体-待测抗原-标记免疫金溶胶"多组分三明治复合体系。

Up to four hydrogen molecules can be attached to the Ti atom and all of them are not dissociative.

在Ti原子附近,最多有四个氢分子可以被吸附,所有的氢分子都处于分子结合态的形式。

The fluorescence spectra of 4- 5 '-p-biphenylyloxadiazolyl-2' benzyl ω-p-biphenylyl polymethylene car-boxylate have been determined. The results of experi-ment show that they can form not only intramolecularexciplex but also intermolecular excimer and triplex.

测定了ω-对联苯基多亚甲基羧酸4-[5'-对联苯基〓二唑-2']苄酯的荧光发射光谱,从实验结果得到,它们不但可以形成分子内激基复合物,而且可以形成分子间激基缔合物和三分子激基复合物。

The seed layer has an intermolecular spacing of 3.4 -0.3 AA. and it is formed by rodlike supramolecules comprised of disc-shaped molecules of at least one polycyclic organic compound with conjugated p-system.

该晶种层具有3.4±0.3的分子间距,并通过由盘形分子构成的棒状超分子形成,该盘形分子至少为一种具有π共轭体系的多环有机化合物。

Were used as parents to produce a F2 population consisting of 120 individual plants, which were used to construct a molecular linkage map. On the basis of observing the qualitative and quantative traits of the map population, Two types of DNA molecular marker methods, simple sequence repeals and amplified fragment length polymorphism, were used to construct a linkage map of molecular markers and to map genes of agronomic characters.

多标记基因系T582和T586为亲本,构建了一个由120个单株组成的用于分子标记图谱构建的F_2作图群体,在考察和分析亲本、F_1和F_2群体质量性状和数量性状的基础上,利用SSR和AFLP分析技术,首先对各群体进行DNA分子标记的多态性分析,然后对显示多念性的标记进行连锁分析、图谱构建和农艺性状基因的定位。

The immune system reacts powerfully to a surprising variety of the patient's molecules, ranging from targets exposed at the surface of cells to some inside of cells to even some within a further sequestering chamber, the cell nucleus.

狼疮患者的免疫系统会对体内许多分子产生强烈反应,种类多到令人惊讶,从暴露在细胞表面的分子到细胞内的分子,甚至是包在细胞核内的分子。

As for epidotesfrom blueschist, X_=0.22--0.36, and their composition varies remarkablyeven.in the same sample. In general, fine--grained epidotes contain relativelylow ps while things are just the opposite for coarse--grained ones whichcommonly exhibit zonal structure characterized by the gradual decrease of pstowards the core, suggesting that the bluescbist might have experienced apressuree--decline metamorphic process.

s=0.22—0.36,其成分在同一样品中也有很大变化,一般细粒者ps分子少,而粗粒者ps分子多,且常发育环带结构,向中心ps分子减少,说明蓝片岩可能经历了一个以降压为特征的变质过程。

Most CD molecules have homologues in other mammalians, of which mouse is the most intensively studied.

多数CD分子在其它哺乳动物中都存在同源分子,其中以小鼠的同源分子研究最多。

GPR39 diverged first, and an ancestral GHSR-like receptor gave rise to a fish specific clade A, GHSR and MLNR by successive gene duplication that occurred before the divergence of tetrapods and fish.

由此他们提出了分子相互作用网络系统进化形成的一种模式:新功能关系形成之前,通常存在基因共享,即一种分子参与到多个过程、多种分子间相互作用。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。