多倍性

In the self-bred progenies of autopolyploid,we found that a very few chimaeraexist in the cells of root tips,but most of them had no change in ploidy. We also found13 diploid plants in the experimental plot (ratio is 0.46%) and can be divided into 4categories.

在部分同源多倍体株系的自交结实后代中，随机取样进行根尖染色体检查，结果表明多倍株系体的自交结实后代倍性没有变化，但发现有个别细胞为不同倍性的嵌合体；田间观察到分离出来的极少数正常二倍体，占0.46％，按照形态特征可以分成四个不同的类型。

These results indicated some differences from other investigated autopolyploid plants in autopolyploid watermelon. Autotriploid watermelon, in particular, showed unusual characters of genome structure and DNA methylation status, which may be closely associated with the oddploidy effect and the excellent traits present in triploid plants.

综上结果证明，西瓜同源多倍化过程中发生的遗传和表观遗传改变与已报导的一些植物的同源多倍化有所不同，特别是三倍体西瓜，其表现出独特的基因组结构及DNA甲基化状态变化特徵，而这可能与植物的奇倍性效应及三倍体西瓜优良性状的形成密切相关。

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

The number of chloroplast in stomata guard cell, density, length and width of stomata were observed.

以苹果和梨的二倍体和多倍体为试材，比较了气孔保卫细胞叶绿体数目、气孔密度、气孔长和气孔宽四个性状与倍性的关系，分析各个性状用于倍性鉴定的可靠性，并采用两类性状同时进行判别分析，建立判别分析方程。

Polymorphism in diploid species G.arboreum was the same as in tetraploid species G.hirsutum,both were 11.84%.

在二倍体棉种中棉的扩增多态性率为11.84%，与在四倍体棉种陆地棉的扩增多态性率相同。

It implies that the relationship between Japanese black cattle and Mongolian cattle is more distant. According to that the rate of sequence divergence of mtDNA is estimated to be 2% per million years, Haplotype II and III of mongolian cattle s mtDNA might be separate about 240 thousand years ago, which is earlier than the 10000-year history of cattle husbandry. Haplotype of yellow cattleand zebu cattle might be separate about 0.73-0.92 million years ago.

按照哺乳动物mtDNA碱基突变率每百万年2％计算，单倍型Ⅱ和Ⅲ(遗传距离为0.478％)所代表的蒙蒙古牛和日本和牛遗传多态性比较研究硕士学位论文古牛群体之间的分化时间大约24万年，这比人类驯化牛10000的历史要久远得多。I单倍型和11、m两种单倍型(遗传距离为1.467%，1.83870)所代表的牛群体之间的分化时间大约73万年至92万年前。

DNA C-values might be one of important indices in the evaluation of angiospermous invasiveness. To test this viewpoint, we compared DNA 1C-values and basic genome sizes in 3,676 angiosperms. The results suggest that:(1) the two nuclear values vary greatly among different groups, e.g., significantly higher in herbs than in trees, in monocots than in dicots, in perennials than in non-perennials;(2) the two nuclear values both have significant effects on plant invasiveness, especially in herbs, dicots, monocots, perennials, non-perennials, diploids, polyploids, Compositae, and Poaceae, while not significant in trees and Fabaceae.

DNA C值与被子植物入侵性密切相关，为考察应用DNA C值评估被子植物入侵性的可靠性和局限性，我们统计分析了全世界范围内3676种被子植物的DNA 1C值（配子未发生DNA复制时的染色体DNA含量）及基因组大小（单个染色体组的DNA 含量），结果表明：1）DNA 1C值和基因组大小在被子植物不同类群中差异极大，例如在草本植物中显著高于木本植物，单子叶植物显著高于双子叶植物，多年生植物显著高于非多年生植物；2）两项指标都与被子植物入侵性显著负相关，尤其是在草本植物、双子叶植物、单子叶植物、多年生植物、非多年生植物、二倍体、多倍体、菊科和禾本科中，然而在木本植物、豆科植物中其与植物入侵性无显著关系。

However, analysis of DNA methylation level indicated that the total DNA methylation levels were not significantly different between diploid and autotetraploid. Autotriploid showed a trend of low DNA methylation level. It implied that the alterations of DNA methylation levels were not linear with the autopolyploidy level in watermelon.

有趣地是，二倍体与四倍体西瓜相比DNA甲基化水准却未发生大规模的调整，而三倍体西瓜显示出较为明显的低DNA甲基化水准趋势，表明西瓜同源多倍化过程中其基因组DNA甲基化水准变化与其倍性不成线性关系。

Using nuclear DNA C-values for 539 angiosperms in China, we examined the variation of these values among growth forms and taxonomic groups and the relationship of these values with invasiveness. Mean DNA C-value of the 539 angiosperm species was 4.06 pg. Mean DNA C-value was(1) significantly lower for woody species (1.84 pg) than for herbaceous species(5.02 pg);(2) significantly lower for 360 dicots (2.20 pg) than for 179 monocots (7.80 pg);(3) significantly lower for annuals (2.78 pg) than for perennials(6.65 pg);(4) significantly lower for 134 weed species (1.93 pg) than for herbaceous non-weeds (6.75 pg) and for several families that have an unusually high proportion of weed species;(5) significantly lower for 47 exotic weed species (1.76 pg) than for 134 native weeds (1.93 pg), but significantly lower than that of "non-weedy" herbaceous species (6.75 pg);(6) lower for weeds than for "non-weedy" species in same genus or family; and (7) in herbaceous species, generally lower for weedy compared to "non-weedy" species, with some exceptions such as Avena fatua, whose DNA C-value is as high as 14.15 pg, contrarily, and some "non-weedy" herbaceous species in Cruciferae and Cucurbitaceae with very low values.

统计了中国境内有分布的539种被子植物的DNA C-值，分析了它们在不同分类群、生活型、倍性、生活史类型以及在杂草和非杂草类群中的分布情况，主要结果如下：(1)539种被子植物DNA C-值平均为4.06 pg，其中木本植物的DNA C-值平均为1.84 pg，低于草本植物的平均值(5.02 pg)；(2)双子叶植物(360种)的DNA C-值平均为2.20 pg，极明显地小于单子叶植物(179种)的平均值(7.80 pg)；(3)1年生植物的DNA C-值平均为2.78 pg，明显小于多年植物的平均DNA C-值(6.65 pg)；(4)134种杂草的DNA C-值平均为1.93 pg，明显小于非杂草草本植物的平均值(6.75 pg)，含杂草较多的科，平均DNA C-值相对较小；(5)统计的47种入侵杂草的DNA C-值平均为1.76 pg，略小于134种杂草的平均DNA C-值(1.93 pg)，极显著地小于非杂草性草本植物(6.75 pg)；(6)以科为单位，不同科的DNA C-值存在着极大的差异；(7)DNA C-值与染色体倍性的关系并不明显，但是，随着倍性的增加，基因组变小；(8)在同一科、属中，与非杂草相比，典型杂草的DNA C-值往往偏小；(9)总体上杂草或杂草性强的植物，它们的DNA C-值比非杂草性植物的要小。

In this study, We typed the the gene of β〓-AR from genomic DNA of asthmatic patients and made the further analysis: The distributing condition of genetic polymorphisms at positions 16 and 27 in β〓 AR coding region and at position 19 in 5'Leader Cistron (5'LC) among southwest Chinese asthmatic population; The relationship between the each polymorphisms at the above three positions and the asthmatic phenotypes (FEV〓 or nocturnal asthma); The effect of each polymorphisms at the above three positions on bronchodilator responsiveness induced by β〓- agonists(initial bronchodilator response, pharmaco-dynamic); The relationship between the haplotypes of polymorphisms in the 5'LC or in positions 16, 27 of β〓AR coding block and asthmatic phenotypes or bronchodilator responsiveness induced by β〓-agonists.

实验通过对哮喘人群的β〓-AR基因进行分型，探讨：β〓-AR编码区16、27位点及5'前导肽(5'LeaderCistron，5'LC)19位点遗传多态性在中国西南地区哮喘人群中的分布情况；三个位点多态性与哮喘临床表现型的关系；三个位点多态性对β〓受体激动剂反应性(支气管舒张反应，舒张反应动力学)的影响；16、27位点及5'前导肽19位点遗传多态性单倍型与哮喘临床表现型及β〓受体激动剂反应性的关系。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐