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The results showed that: at metaphase I, the homoeologous chromosome pairing among different F1 hybrids ranged from 2.0 to 11.4 bi-valents formed by homoeologous chromosomes per pollen mother cell, and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva-lents were disjoined and most univalents were divided.

结果表明在中期I阶段,这些杂种一代的近缘染色体联会变化很大,每个花粉母细胞中二价体形成的数目从平均2个到11.4个不等,甚至在某些花粉母细胞中,还发现极少的多价体和非部分同源染色体所形成的单基因组内二价体;在后期I时,所有的二价体分离,同时多数单价体也分离,分离的二价体和分离的单价体都移向两极,从而形成两组染色体;因为这时完整花粉母细胞中分离的二价体在两组染色体中总是对应出现,从而根据半二价体上染色体重组的位置可以分析在二价体的四分体时期发生在非姊妹染色体之间的多种染色体交换类型,如单交换、三线双交换、四线双交换、四线三交换和四线多交换。

In the absence of coorperative effect, the reaction between lectin molecules and their receptors on cell surface can be described as below when equilibrium is reached:In which LT denotes total lectin concentration, LF free lectin concentration, mva-lency of lectin, ni number of receptors with affinity constant Ki to lectin, CT total lectin concentration.

本文提出一个描述细胞与多价凝集素反应的平衡关系式和用紫外分光光度法测定游离凝集素浓度求受体数量及其与凝集素的亲合常数的方便方法。

Under normal temperature (25-30℃), the process of pollen mother cell meiosis is normal; The most of chromosomes become bivalents which like "V","O","Y","X", sticks and dots, and mainly like dots,"V" and "O"; and a few of univalent (0.79% of Tainong 1 and 0 of Irwin) and multivalent (4.76% of Tainong 1 and 3.57% of Irwin) had been observed.

常温(25~30℃)条件下,花粉母细胞减数分裂过程较为正常,双线期染色体主要形成二价体,二价体构型以&V&型、&O&型、&Y&型、&X&型、点状和棒状等形式存在,并以点状、&V&型和&O&型较多见,出现少量的单价体和多价体,其中台农1号为0.79%和4.76%,Irwin为0和3.57%;同时仅在少量中期Ⅱ和后期Ⅱ花粉母细胞中观察到落后染色体。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样,利用PCR和SOE技术,获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因,以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗:二基因融合(pCDNA3.1-MPB64-Ag85B,简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6,简称pCMAE)DNA疫苗;二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗,免疫BALB/c小鼠,以牛分枝杆菌BCG免疫组为阳性对照,以pCDNA3.1及PBS免疫组为阴性对照,共免疫3次,每次间隔2周,BCG组仅初免时皮下免疫1次。1免后每周,以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原,以间接ELISA方法检测血清IgG水平及lgG亚类。

The omega fatty acids are the major building blocks of cellular membranes, and they keep the cell membrane optimally fluid. The omegas can convert to hormone like substances that influence metabolic activities.

多价不饱和脂肪酸是维持细胞膜结构和功能的必要组分,也是合成其它生理活性物质的前体化合物。

Objective To determine the levels of the long chain polyunsaturated fatty acid in preterminfants and its influencing factors.

目的 探讨早产儿出生时长链多价不饱和脂肪酸水平及其相关因素。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。