多义的

The traditional approach classifies antonyms into root antonyms and derivational antonyms from morphological point of view and the specific semantic features reflected in antonymy are neglected.

传统的反义关系理论以词根和派生依据，将反义词划分为词根反义词和派生反义词。这一划分标准更多着眼于词汇形态，而忽视了反义关系的具体语义特征。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

However, multiple stable nonsynonymous mutations were detected gradually during 50 passages of 4 serials in embryos with maternal antibody to LG1 strain, and the NS/S ratio was as high as 3.46 among 45 mutated sites.

在2 个传代系列的10 个代次病毒，共出现了29 个位点变异，有义突变与无义突变比值NS/S为1.42 。但在有抗体的鸡胚的传代过程中，发生了多个呈现稳定遗传的有义突变。

Taiwan Yi Heng Industrial is a world-renowned systems and surface equipment Flooring Materials Chemical manufacturers (the main launch in mainland China Products: Flooring wiped-ray machines, stone grinding machine, shot blasting machine, grinding machines, milling machines, plate ram, cutting machine, marking machine, all kinds of supplies and epoxy resin, etc.), powerful, with a number of outstanding technical expertise, thirty years,"Yi-hang" because of its high quality, reasonable price and excellent service grown into a well-known brands that have an impact, Yi-Heng products to more than 30 countries or regions, different levels of user access to wide acclaim and widespread recognition.

台湾义恒工业是世界著名的表面设备系统和地坪化工材料制造商（在大陆主要推出产品：地坪抹光机、石材研磨机、抛丸机、磨削机、铣刨机、平板夯、切割机、刻纹机、各种耗材及环氧树脂等）、实力强大，拥有一批出类拔萃的技术专才，三十多年来，&义恒&因为其高品质、合理的价位和优良的服务成长为一个具有影响的著名品牌，义恒的产品也走向三十多个国家或地区，获得不同程度的用户广泛赞誉和普遍的认可。

Hyponyms of the same super-ordinate are co-hyponyms to each other.

同一个上义词的多个下义词叫并列下义词。

A type has 7 point mutations(5 samples). B type has 6 point mutations (3 samples), C type has 4 point mutations (3 samples) and D type has 2 point mutations(1sample). Acid amino alignment shows that the most mutations of gastric cancer are mis-sense mutations, few ones are synonymous mutations. Most of the mutations occurred in regulating domain down to the HMG-box,only 2 sites in HMG-box.The mutations have same sites in middle and late stage samples. The results revealed that SOX4 mutations are associated with gastric cancer,which is possible a late accident in gastric cancer.

通过对相应的氨基酸序列的比对发现，发生在中晚期胃癌中的突变多为错义突变，同义突变不多，且突变大多发生在HMG盒下游编码区，在HMG盒内的突变只有两处，中期胃癌与晚期胃癌的突变有相同之处，但SOX4基因的突变位点随肿瘤的演进有增加的趋势。

The Italian American people of Wilmington, Delaware, celebrate St. Anthony and 11 other saints with the St. Anthony of Padua Feast Day Procession. This annual religious procession, which celebrated its 75th anniversary in 2000, is a reminder of their Italian heritage and faith.

德拉瓦州威明顿市的义裔美人，会透过帕多瓦宴会日的圣安东尼游行，来颂扬圣安东尼及另外的11位圣徒；这个一年一度的宗教游行，在2000年时欢渡了它的75周年庆，它是这些义裔美人用来提醒他们的义大利常规及信仰的庆典。

The Italian American people of Wilmington, Delaware, celebrate St. Anthony and 11 other saints with the St. Anthony of Padua Feast Day Procession. This annual religious procession, which celebrated its 75th anniversary in 2000, is a reminder of their Italian heritage and faith.

德拉瓦州威明顿市的义裔美人，会透过帕多瓦宴会日的圣安东尼游行，来颂扬圣安东尼及另外的11位圣徒；这个一年一度的宗教游行，在2000年时欢渡了它的75周年庆，它是这些义裔美人用来提醒他们的义大利传统及信仰的庆典。

The Italian American people of Wilmington, Delaware, celebrate St. Anthony and 11 other saints with the St. Anthony of Padua Feast Day Procession. This annual religious procession, which celebrated its 75th anniversary in 2000, is a reminder of their Italian heritage and faith.

特拉华州威明顿市的义裔美人，会通过帕多瓦宴会日的圣安东尼游行，来颂扬圣安东尼及另外的11位圣徒；这个一年一度的宗教游行，在2000年时欢渡了它的75周年庆，它是这些义裔美人用来提醒他们的义大利常规及信仰的庆典。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐