外膜

Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

The effects of racemate initial concentration, volume ratio of surfactant to organic solvent, pH value in internal or external phase, pH gradient from external to internal phase, ligand metallicion and system temperature on the performances of chiral separation were discussed.

摘 要：研究在含有Cu2+和N-n-十二烷基-L-脯氨酸手性配体的乳状液膜体系中手性萃取分离氧氟沙星对映体的效果，以及外消旋体的初始浓度、表面活性剂与有机溶剂的体积配比、内外水相pH值、从外水相到内水相pH值梯度变化以及配位金属离子与体系温度对液膜手性分离性能的影响。

So we propose that before the precursor is cleaved into DG and mediator, it is released from plasma membrane first, through activating some kind of enzyme by insulin or PIPLC; meanwhile, only insulin can activate the resynthesis mechanism of the precursor, in the contrast, PIPLC was inactive at this course.

推测：胰岛素或PIPLC作用于完整细胞或膜时，激活了某种酶，使前体先从膜上释放至胞外，再被水解为介体和DG，同时胰岛素还能激活再合成前体的机制，而PIPLC却不能。

Based on the low Reynold number and small flux model, the purpose of the paper is to study the velocity and thickness of the liquid film outside the horizontal tube.

本文采用低雷诺数小流量模型对粘性流体在水平管外形成液膜的流速以及膜厚分布进行分析和计算。

Based on the low Reynold number and small flux model, the velocity and thickness of the liquid film outside the horizontal tube were studied.

结果表明：重力驱动在光滑横管外壁面白发形成均匀的液膜是不可能实现的，必须外加一个能产生特殊流场的气刀来改变其原有受力情况，才有可能实现均匀液膜。

（1） In common cavity malformation, in addition to the abnormal bony labyrinth such as the cochlea, vestibule and lateral semicircular canal, the inner ear perceptive organ such as the saccule, utricle and scala media were also abnormal.（2） Though we could not identify the basal membrane and sensory epithelia under micro-endoscopy, the utricle and saccule could be clearly identified.（3） During cochlear implantation in common cavity malformation, electrode insertion should be monitored under micro-endoscopy, the electrodes should be put close to the anterior wall of the cavity, otherwise the vestibular organ may be damaged.

（1）共同腔畸形极重度聋患者不仅前庭、外半规管和耳蜗的骨性结构形态发生了变化，而且腔内耳蜗中阶与前庭的球囊、椭圆囊的膜迷路结构形态也发生了变化；（2）微窥镜下分辨不出基底膜或听神经细胞的形态结构，可见畸形前庭的球囊、椭圆囊的囊性结构；（3）进行共同腔畸形人工耳蜗植入手术时，应在耳蜗内镜的监视下，将电极摆放到准确位置，尽可能贴近共同腔的前壁，不应损伤前庭器官。

Apart from the above shrinkproof pore-class, there is also an surfactant with non SI fluorin Department of surfactants, which are the monlchamus membrane porosity, and enhancing and improving the leveling business card printing and membership card production ink film luster is also very effective.

除了上述防缩孔剂类外，还有属高分子表面活性剂的无硅的氟系表面活性剂，它对于防止墨膜的缩孔，增进流平及提高制卡和会员卡制作墨膜的光泽度也十分有效。

Hematoxylin-Eosin dying method is used to discover the microstructure of the spermatheca by light microscope.

采用HE法对中华蚌锰受精囊的显微观察显示，受精囊的囊壁结构由内向外可分为内膜层、上皮层、基膜、肌肉层、结缔组织、围脏膜六层。

It is suggested that the action site of ABA on sugar unloading may be at theplasmalemma of SE/CC complex, but not at the apoplasmic space during sugar metabolism indeveloping apple fruit,and that the action site of ABA on sugar loading into sink cells may be notrestricted only at the plasmalemma of the cells.

根据实验结果综合分析认为，ABA对果实内糖卸出的作用位点可能在SE/CC复合体的质膜上，而不在质外空间糖的代谢过程中；ABA对糖装入的作用位点可能不限于库细胞质膜；ABA在果肉韧皮部的富集，一方面可能与ABA的输入有关，另一方面可能与韧皮部存在ABA作用位点有关。

On the basis of study on the conditions and pathways of expression of cell totipotency , totipotent cells were inquired to found the clone of marine macroalga.

依据藻体类型和早期形态发生类型，选择假膜体、膜状体和多核单细胞体三种类型的海藻，在单藻培养、不加激素的条件下进行外植体培养，研究海藻细胞全能性表达的条件和途径，并在此基础上，寻找全能性细胞，构建大型海藻无性系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。