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Objective: To assess the role of NADPH oxidase-related oxidative stress in the intimal hyperplasia induced by adventitial injury.

目的:探讨NADPH氧化酶相关的氧化应激在外膜损伤致内膜病变中的作用。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度;CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段;微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率,同时具有一定的靶向性,但总的转染范围仍然有限;采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预,发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低,CaN AβmRNA水平虽有下降趋势,但无统计学差异,提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Immunofluorescence staining shows that, the CaN Aβ protein expression of intrapericardial injection group decreased in some myocytes under epicardium, while other groups show no decreased fluorescence.

三在体心肌CaN AβsiRNA表达质粒转染后,转染各组与心肌肥大模型组CaN AβmRNA水平比较无差异(P>0.05),但免疫荧光结果显示心包腔内途径CaN Aβ干扰组较心肌肥大模型组心外膜下部分心肌荧光减弱。

However, none of these therapies can manage this complication perfectly. Different from complete ureteral avulsion, when mucosal avulsion happens, the ureteral muscular layer and membrana externa remain their continuity.

这些方法基本遵循了输尿管全层撕脱、断裂或部分缺损的处理原则,而忽视了输尿管粘膜剥脱时,肌层和外膜尚保持着良好的连续性这一现象。

The periosteum of the femoral Metaphysis in aged rat, the RANK mRNA expressed was less than those in the bone of 12-week-old rat, in the trabecular bones, the expressing of RANK was stronger in the ovariectomized rats than in the sham-groups.

在老年雌性大鼠的股骨干骺端皮质骨外膜中RANK的表达较12周鼠龄的股骨干骺端中RANK的表达减弱,骨内膜中RANK的表达在不同的鼠龄中变化不明显。

Here we show, for eight heterosexual transmission pairs, that recipient viruses were monophyletic, encoding compact, glycan-restricted envelope glycoproteins.

作者发现在8对异性传播例子中被感染者病毒是单源的,编码紧凑的聚糖-限制型外膜糖蛋白。

Mooth muscle actin highlights pericytes surrounding the endothelial cells.

MA染色可突出显示内皮细胞周围的外膜细胞。

Endocericosis is an uncommon mullerian lesion consisting of irregularly-shaped glands proliferating deep in the bladder wall.

膀胱宫颈内膜异位症是一种较少见的mullerian病变,患者常有剖腹产病史。病变主要在膀胱肌层,粘膜及外膜也可受累。

Pleomorphic/nontolerated VTs required electroanatomic and noncontact mapping in 48 and 22 patients, respectively, and percutaneous cardiopulmonary support in 10 patients.

在多形性或不能耐受的VT患者中,48例患者进行电解剖标测,22例患者行非接触标测,10力患者接受经皮心肺支持,10例患者行心外膜途径消融。

Pleomorphic/nontolerated VTs required electroanatomic and noncontact mapping in 48 and 22 patients, respectively, and percutaneous cardiopulmonary support in 10 patients. An epicardial approach was used in 10 patients.

在多形性或不能耐受的VT患者中,48例患者进行电解剖标测,22例患者行非接触标测,10力患者接受经皮心肺支持,10例患者行心外膜途径消融。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。