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This experiment was carried out to provide reference for reasonable selection of pH and phosphate levels when phosphorus absorption experiment in vitro with Chicken small intestine by investigate the effects of phosphate levels and pH in culture medium on absorption of Pi by incubating everted intestinal sacs of broilers.

探讨了培养液不同pH和磷含量对鸡离体小肠磷吸收的影响,为体外培养鸡小肠合理选择培养液pH和用外翻肠囊法评定饲料有效磷时合理选择培养液磷浓度提供参考依据。

This reserch was studied on the Breviscapine intestinal absorption dynamics first-time with 3 methods : everted sac technique, body circulatory perfusion technique and Caco-2 cell method.

本研究首次运用外翻肠囊法、在体肠循环灌流法和Caco-2细胞模型法研究了灯盏花素的肠吸收动力学。

A quantitative assay of OM and M in biological specimen was established on their characteristics Everted gut sac and Caco-2 cell model were used to study the intestinal absorption kinetics of OM and M.

通过大鼠外翻肠囊和Caco-2细胞这两种肠吸收模型对OM和M的肠吸收特征及吸收动力学进行了研究,两种方法都显示OM的肠吸收主要为经细胞旁路被动扩散吸收,而M的肠吸收主要为经细胞通路被动扩散吸收。

ABSTRACT This research was studied on the Breviscapine intestinal absorption dynamics first-time with 3 methods: everted sac technique, body circulatory perfusion technique and Caco-2 cell method.

本研究首次运用外翻肠囊法、在体肠循环灌流法和Caco-2细胞模型法研究了灯盏花素的肠吸收动力学。

objectiveto study the effects of glycyrrhizin on intestinal absorption of paeoniflorin by the everted rat gut sac model.methodsused a simple rp-hplc method for the determination of paeoniflorin in the krebs fluid.

目的研究甘草酸对芍药苷大鼠肠吸收的影响。方法采用大鼠肠外翻模型研究芍药苷的肠吸收,并用反相高效液相色谱法测定大鼠肠黏膜内外两侧的芍药苷浓度。

The results suggest: the optimum pH is 6.0~7.0 and phosphorus content can not exceed 200μg/ml in culture medium when assessefeed available phosphorus with method of everted intestinal sacs.

结果提示:用外翻肠囊法评定饲料有效磷时,鸡离体小肠培养液pH以6.0~7.0最佳;培养液中磷含量不能超过200μg/ml。

Methods: The Ostα gene was inactivated by conventional gene targeting. Mucosal-to-serosal taurocholate transport was measured in vitro using everted gut sacs.

使用传统的基因打靶技术将Ostα基因灭活,通过外翻肠囊实验在体外检测牛璜胆酸从粘膜到浆膜的转运。

The result of everted sac technique test showed the way of Breviscapine intestinal absorbtion is passive transport mechanism and maximum absorptivity varied from 13.1% to 16.9%.

外翻肠囊法研究结果表明,灯盏花素在肠道的吸收方式属于被动扩散吸收,最大吸收率从13.1%~16.9%。

ConclusionThe results suggest: the optimum pH is 6.0~7.0 and phosphorus content can not exceed 200μg/ml in culture medium when assesse feed available phosphorus with method of everted intestinal sacs.

结果提示:用外翻肠囊法评定饲料有效磷时,鸡离体小肠培养液pH以6.0~7.0最佳;培养液中磷含量不能超过200μg/ml。

Results The observation shows this method is effective for prevention of postoperative bleeding, short healing time, less incision ache, also no complications of rectal narrow, incontinence and mucosa everting and so on.

结果 该术式术后预防出血效果明显、愈合时间短、切口疼痛轻微、无肛门狭窄、失禁及粘膜外翻等并发症。

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