外甲

Treatment with amydae carapax decoction could resist lipid perioxidation, improve pathological function of liver tissue, improve liver function and regulate cell factor level. Thus it could inhibit hepatic stellate cells activated multiplication and extra cellular matrix synthesis and secretion, promote ECM degradation and significantly prevent and treat liver fibrosis in rats.

鳖甲煎煮液能有效地预防和治疗大鼠肝纤维化，效果显著，其作用机制可能是通过抗脂质过氧化、改善肝组织病理、改善肝功能、调控细胞因子水平等而发挥抑制肝星状细胞活化增殖及细胞外基质合成分泌、促进ECM降解吸收等综合作用，阻断和治疗肝纤维化。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Different treatment regimens had been designed according to different classification.②Properly combined chemotherapy protocols reduced therapy intensity and decreased drug toxicities to patients with standard risk ALL.High dose methotrexate,instead of craniospinal radiation,had become effective phylaxis,and decreased the incidence of extramedullary relapse and CNSL (if indispensably,radiation dose was reduced).This measurement reduced radiation chemotherapy long term side effects and improved survival quality.

化疗方案合理组合在保证疗效的同时对ALL后期治疗降低强度，减少药物毒性，同时改进了庇护所预防措施———采用大剂量氨甲蝶呤，大大减少了髓外复发，使相当一部分患儿放弃以往颅脑预防性放疗，即使需要放疗者也降低了剂量，从而减少放化疗远期毒副作用，提高了生存质量。

Result:In most cases, the recurrent laryngeal nerve diverges at the level of 1.0～2.5 cm under the cricothyroid articulation for forming the extralaryngeal division.

结果：95%喉返神经在距同侧环甲关节下方1.0～2.5 cm处形成喉返神经喉外分叉点，根据分支情况可将其分为三型。

The passive diffusion is the major transport of daphnetin uptake and absorption by Caco-2 monolayer.P-gp has no efflux effects on the uptake and transepithelial transport of daphnetin.

祖师麻甲素是以被动扩散为主要方式被小肠上皮细胞摄取和转运，此过程不受P-gp的外排作用。

ABSTRACT Pachyonychia congenita is an autosomal dominant genodermatosis characterized by ectodermal dysplasias, of which the hypertrop...

先天性厚甲症(Pachyonychia congenita，PC)系外胚叶缺陷病，是一种常染色体显性遗传性疾病。

The mechanism of outside cell reaction of electrosynthesis of p-methoxybenzaldehyde and p-bromobenzaldehye is studied by Fourier-transform infrared absorption spectra.

利用红外光谱研究了槽外合成对甲氧基苯甲醛和对溴苯甲醛的反应机理，结果证明：在Ce4+与对取代甲苯的氧化反应中，苯环上甲基被Ce4+氧化的难易受苯环上对位取代基的影响。

This protocol could also be used for the selective reduction of the endocyclic double bond of the 3-electron withdrawing group substituted coumarins. This method is clean, mild, and efficient.2 ZrCl_4 / HEH was found to be a selective and efficient reagent combination for the direct reductive amination of electron rich aldehydes and ketones with weakly basic amines after scanning various catalysts such as InCl_3, BiCl_3, CF_3COOH and so on.

图一 1。我们用汉斯酯作为有机还原剂，乙醇为溶剂，实现了4-甲叉基噁嗯唑酮和吡唑酮环外双键的高选择性还原；此外，我们还将这一还原体系用于3-吸电子集团取代-3，4-二氢香豆素环内双键的选择性还原。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。