外物

In addition, the compound does not appear to be a substrate for P-gp efflux, resulting in a relatively long duration of reversal activity in resistant cells even after the modulator has been withdrawn.

另外，所述化合物并未表现出为P-gp外排的底物，导致逆转活性在抗性细胞中具有相对长的持续时间，即使是在调节物被撤去之后。

At the same time, PEX tubing is outward expansion, AEI complex - British PEX pipe complex of professional businesses, are kent state of its 9000 square meters of new production technology operations center.

同时，PEX 管材正在向外扩张，AEI复合物--英国PEX 管材复合物专业企业，正在kent州新建其9000平方米的生产技术运营中心。

The 20S catalytic core of the 26S proteasome has the shape of a barrel made of four rings which are composed of seven differentαsubunitsα_1-α_7 at two outer rings and seven differentβsubunitsβ_1-β_7 at two inner rings at which the catalytic sites locate. Three of theβsubunits contain proteolyses sites, which are sequestered in the hollow interior of the 20S particle. Substrates enter the 20S through a narrow channel formed by theβsubunits, whose N-termini, depending on their conformation, can either obstruct or allow substrate entry and thus function as a gate. This entry channel is narrow and only permits passage of unfolded, linearized polypeptides.

哺乳动物26S蛋白酶体是由一个20S催化颗粒(catalytic particle，CP)和两个19S调节颗粒(regulatory particle，RP)组成的ATP(adenosine-triphosphate，ATP)依赖性蛋白水解酶复合体。20S CP是26S蛋白酶体的催化核心，它是由4个圆环堆叠形成的桶状复合物，其中两侧外环每个环是由α_1-α_7亚基组成，两个内环每个环是由β_1-β_7亚基组成，4个环的中央形成一个狭窄的内腔。2个β内环形成了20S CP的催化中心空腔，其内壁为催化活性位点所在地；外侧α环中心的孔道是底物到达催化中心空腔的通道，一般被α亚基上的N末端所封闭，阻止胞内非目的靶蛋白进入20S CP内被降解破坏。

Sediment from river is not the main source of the Rushan Bay now any more ,which leads to the change of balance of sedimentation power. Sediment will transport because of the drive of hydrodynamic elements in macroscopic view .Direction of suspended load is basicly the same as tidal current .Flood tide makes suspended load transport to tip of the inner Bay and to the Mouth in exterior Bay .The direction of longside sediment transport in two sides of the Mouth mainly focus in the Mouth .Because the coastline along the exterior Bay is very meandering and it belongs to the type of bedrock ,besides some sediment going into the inner Bay ,so actual net sediment discharge rate is less than calculation .It develops an ebb delta out of the Mouth and forms tidal sand ridge ,tidal washing canal ,barrier bar and marine physiognomy ;The result of model simulation disclosures that differences of sediment erosion and siltation between single tidal action and wave-current action are very big .From the analysis of sediment composition、sediment sources and stability of beach and ridge ,this article gets the conclusion that the channel has the possibility to silt ,though the course maybe very slow.

通过分析研究文章得出如下结论：沉积物平面分布与水动力的时空变化存在很好的相关性；口门外发育落潮流三角洲，形成潮流沙脊、潮流冲刷槽、浅滩、拦门沙等水下动力地貌；乳山湾河流来沙已不是湾内泥沙主要来源导致沉积水动力平衡发生改变，宏观上表现在泥沙受水动力驱动发生不断运移；悬沙运移方向与涨潮流方向基本一致，湾内涨潮流带来的悬移质泥沙向湾顶运移，湾外悬移质泥沙主要向口门方向运移；湾口两侧岸段沿岸输沙方向均指向口门方向，由于乳山湾外海岸线曲折，两侧岸段以基岩为主，加之部分泥沙进入湾内，实际净输沙量小于计算值；数值模拟发现潮流单独作用与波流共同作用下泥沙蚀淤模拟差别较大；从沉积物组成、泥沙来源及滩槽稳定性三个方面分析，文章认为研究区存在航道淤积的可能性，但过程相对较慢。

The gonadotropin releasing hormone receptor gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 1, exon 2 and exon 3 of GnRHR gene in both high fecundity breeds and low fecundity breeds (South African Mutton Merino, Corriedale and Chinese Merino sheep) by PCR-SSCP. Only the products amplified by primers P4 and P7 displayed polymorphisms.

设计8对引物，应用聚合酶链式反应-单链构象多态性技术检测促性腺激素释放激素受体（gonadotropin releasing hormone receptor， GnRHR）基因外显子1、外显子2和外显子3在高繁殖力品种和低繁殖力品种（南非肉用美利奴、考力代和中国美利奴绵羊）中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Third, Yangming criticized the learning of Zhu Xi "forgot and kept from the inside" and zen studies "forgot and kept from the outside". At the same time, he insist on a new philosophical position uniting inside with outside, which based on inosculating mind and things.

其三，阳明在批评朱学&务外遗内&和禅学&是内遗外&的同时，坚持以超越内与外、整合心与物的&合内外&作为自己新的哲学立场。

Through research and analysis of data, it was observed that the length and width of bark fiber varied according to the lop and age, the results show the fiber of the Salix bark is longer than the Salix and width of fiber almost same, fiber length of the Salix bark mainly influenced by the times of lop. The ash in Salix bark,1% Sodium Hydroxide Solubility in Salix bark, water solubility in Salix bark, Ethanol-Toluene in Salix bark, acid-insoluble lignin in Salix bark changed with the lop and age is also been investigated, the component of Salix bark is higher than Salix except acid-insoluble lignin,and it's high effected by times of lop; the TG analysis and DSC analysis is been used on the Salix bark and Salix to study the character of the hot-action, and the rang of the pyrogenate temperature is confirm; the quantity of Salix bark effect on the MOR, MOE, swelling in 24h water, nail withdraw and inner strength was determined, the results show that the Salix bark notability effect on the properties of board and dropped it; the properties of the Salix bark board is nearly the board product by other bark residues, it was established the multi regression equation of each properties and optimized the fitting produce condition of Salix bark board.

通过试验和数据处理，得出了沙柳树皮纤维长度、宽度随平茬和材龄的变化规律，结果表明沙柳树皮长度要比沙柳材的要长，宽度基本相同，沙柳树皮纤维长度主要受平茬次数的影响；找出了沙柳树皮中灰分、冷热水抽出物为、1%NaOH抽出物为，甲苯乙醇抽出物、酸不溶木素与平茬次数和材龄之间的关系，除酸不溶木素外，其他成分均明显高于沙柳材，沙柳树皮的化学成分主要受平茬次数的影响；利用TG和DSC法分析了沙柳树皮和沙柳材的反应热特征，确定了热解的温度范围；研究了沙柳树皮量对于刨花板MOR、MOE、吸水厚度膨胀率、握钉力以及内结合强度的影响，结果证明沙柳树皮量对于刨花板的各项性能指标影响显著，它的掺入降低了刨花板的各项性能；对沙柳树皮板的研究表明，沙柳树皮板的各项性能与同类树皮板的性能基本相近，建立的二次回归模型稳定，并利用回归方程求出了适宜的沙柳树皮板制造工艺。

The five prosthetic groups (FAD, [2Fe-2S], [4Fe-4S], [3Fe-4S] and heme group) required for electron transfer from succinate to ubiquinone were unambiguously assigned into the electron density map. Besides, we find there are some electron densities around the Qp pocket at the matrix side and we believe it represents the head structure of ubiquinone, which is proved by the inhibitor bound structure that 2-TTFA just locating at the Qp site. At the same time, we find there is the second 2-TTFA binding site, locating the inter-membrane side. This finding will change our knowledge about the electron transfer inside Complex II and the ubiquinone transfer between Complex II and Complex III, and endow a new role of Complex II in electron transfer chain.

除了对各个电子传递体（ FAD ，[2Fe-2S]，[4Fe-4S]，[3Fe-4S]以及血红素分子）进行精确定位外，我们在该结构跨膜区靠近线粒体基质一端的口袋 Qp 中，发现了一些电子密度，认为是所结合的辅酶 Q 的头部结构，这一点被与抑制剂结合的复合体的结构所证明，在该结构中， 2－ TTFA 恰好结合在口袋 Qp 中，同时，我们还发现了第二个2－ TTFA 的结合位点，位于跨膜区靠近线粒体膜间隙一端的口袋 Qd 中，这个发现具有全新的意义，将影响人们对电子在复合物 II 中传递以及辅酶 Q 在复合物 II 与 III 之间转移的认识，促使人们重新复合物 II 在线粒体呼吸链中的角色。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。