外物

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

Results Of the 5 cases of internal fixation failure,2 were caused by operative mistakes, 1 by wrong selection of internal fixators, 1 by too early weight-bearing,and 2 by postoperative infection;of the 11 cases of nonunion or delayed union, 5were caused by severe primary injury, and 4 by unsuccessful reduction.1 cases of pain were caused by evection of proximal LISS plate,and 1 postoperative infection due to improper emplacement of the plate during the operation resulted in long exposure of plate end.

结果：5例内固定物失效的病例中，2例由于手术操作失误，1例由于内固定物选择失误，1例由于术后负重过早，1例由于术后感染导致内固定物失效；11例骨折不愈合或延迟愈合的患者中，5例由于原始损伤严重，4例由于术中复位不佳引起不愈合或延迟愈合；1例LISS钢板近端突起于皮下引起疼痛，术后伤口感染，1例钢板近端长期外露，是由于术中钢板的放置位置不良；膝外翻畸形1例。

The biocatalyzed characters of the purified cellulases were investigated with the CMC-Na as substrate. The most appropriate catalyzed temprature for cellulase 1 is 60 "C,and 65 for cellulase 2. The two enzymes have the extreme stability at the temperatures no more than 50.The CMC-Na has the protective effect on the cellulase. The pH stability range of cellulase 1 is 4 ~ 8, and 6-9 for cellulase 2. Zn2+, Ca2+, Mg2+, K+, Li+can ativate cellulase 1. K+, Li"1 can ativate cellulase 2. Cellulase 1 can mainly decompose CMC and salicin. Cellulase 2 can decompose not only CMC and salicin, but other substrates feebly.

以羧甲基纤维素为底物时，酶1的最适催化温度为60℃，酶2的最适催化温度为65℃，在50℃以下稳定性较好，底物对酶有较强的保护作用；酶1和酶2的最适pH分别为5.5和5.5～6.0，酶1的pH稳定性范围为4～8，酶2的稳定性范围为6～9；Zn~(2+)，Ca~(2+)，Mg~(2+)，K~+，Li~+对酶1有激活作用，K~+，Li~+对酶2有激活作用；酶1主要对CMC和水杨素有分解作用，对其它底物几乎不分解；酶2除了可以分解CMC和水杨素外，对其它底物也有微弱的分解作用。

The spectroscopic and electrochemical studies suggest the energy structure of this class complex can be easily controlled by modification. With the introduction of electron-donating arylamino groups, the obtained complexes show intense and continuous absorption bands in the region of 250-550nm with strong metal-to-ligand charge transfer dπ(Pt →π* transitions (ε~ 2×104 dm3 · mol-1 · cm-1). Complexes involving the construction of D-π-A type systems also display continuous absorption bands in the region of 400-750nm, rising from the overlapping effect of MLCT and LL'CT bands.

其中，芳胺及其二聚体类修饰基团的引入，使其配合物MLCT吸收谱带的摩尔吸光系数增加，并在250～550nm范围内形成了强的连续吸收；具有D-π-A结构的配合物，由于MLCT吸收谱带和LL'CT吸收谱带的协同效应，导致在400～750nm范围内形成连续吸收；得到了从绿光到深红光的磷光发射系列配合物，并采用真空蒸镀的方法获到了外量子效率分别为5.7%和5.0%的橙光和橙红光多层电致发光器件。

They are:In the third chapter, the crystal structures of these new dca complexes are described anddiscussed in detail. Besides two mononuclear complexes, 1 and 2, complexes 3～15 arecoordination polymer with novel and interesting structural characteristics. 3, 6, 7, 8, 9 are 1Dpolymers, 4 and 5 are of 2D extending structure, while 10, 11, 12, 13, 14, 15, 16, 17 form thefirstly reported series heteronuclear dca coordination polymer, in which (except 10) differentbuilding blocks, with different metal ions in them, construct interesting architecture, like α-and β-type 〓 chains, ladder chain 〓, scaffold structure of 12, and "sandwich"layer structure in 15 and 16, etc..

这些配合物是：第三章中，我们详细描述讨论了这些新型的二氰胺配合物的晶体结构，除两个单核配合物1和2之外，其余十五个为配位聚合物并具有新颖和有趣的结构特征。3，6，7，8，9是一维配位聚合物，4和5则呈现二维扩展结构，而10，11，12，13，14，15，16，17属于首次报道的一系列的二氰胺异金属配位聚合物，其中(除10外)含有不同金属离子的构造&砌块&可构建出有趣的结构，如α-和β-型〓链，梯形链〓，12中的脚手架结构，以及15和16中的&三明治&层状结构等。

These compounds are of substantial interest both inviewpoints of theory and practical applications, mainlyunder the stimulus to achieve synthetic models either foractive sites of iron-sulfur proteins or for transitionmetal catalysts. This thesis describes the synthesis, structures andconformations of 51 new iron-sulfur cluster complexes,which have been characterized by elemental analysis andstandard spectroscopic methods. In addition, single-crystalX-ray diffraction analyses were performed for 4 representa-tive new compounds. Reactivities of μ-〓 with mono- and di- Grig-nard reagents derived intermediates toward various organicelectrophilic substrates were studied, along with complexa-tion of arynyl-containing iron-sulfur clusters by 〓Thus, several bridged, non-bridged, monomeric, dimeric,homonuclear, heteronuclear iron-sullfur and iron-sulfur co-balt-carbon cluster complexes were synthesized. A novelreaction and convenient route for the synthesis in highyield of tetranuclear iron complexes 〓 containing atetracoordinated sulfur atom was discovered, whereas aplausible mechanism based on experimental results was propo-sed for the formation of the new cluster type.

鉴于有机铁硫原子簇化学在基础理论发展和应用方面的重要意义，因此我们开展了这一课题的研究，并取得以下主要成果： 1、本文共合成了51个新有机铁硫及含钴碳簇基的有机铁硫原子簇配合物，除用元素分析，NMR，IR，MS法表征其结构和构象外，还用X-光衍射技术测得四个代表性配合物的单晶结构和构象； 2、我们业已发现炔基Grignard试剂同〓可形成一种&开环&与&闭环&中间物的平衡混合物，研究该平衡混合物同各种亲电试剂的反应； 3、研究发现了一种可高产率地形成〓含〓四配位硫挛合簇的新化学反应并对此反应提出了—种有趣的反应机理； 4、首次由1，4-二溴丁烷双Grignard试剂与〓制得一种新型铁硫簇双硫阴离子活性中间体，并进而研究了它的亲核反应性能； 5、找到了通过〓的光化学反应来合成簇合物〓-〓的一条有实用价值的新路线。

Analysis of the dynamic process of core degradation, debris relocation within the reactor vessel, molten pool formation, reactor vessel melt-through and corium relocation into the core catcher is executed with the computer codes SCDAP/RELAP and MELCOR, and the reaction among the corium, the sacrificial material, and the metal material, as well as the thermal and hydraulic characteristic of heat exchanger is experimentally studied.

在田湾核电站堆芯捕集器的设计中，综合采用了压力容器外包容装置、非能动供水冷却堆芯熔融物包容体金属表面以及用&牺牲性&材料改善熔融物特性和降低热流密度等项技术；利用SCDAP/RELAP和MELCOR两个独立的程序包分析了压力容器内堆芯的损坏、碎片的分布、熔池的形成、压力容器熔穿和熔融物转移到堆芯捕集器等的动态过程，并对堆芯熔融物、&牺牲性&材料、金属材料等之间的物理、化学反应和热交换器的热工水力特性进行了实验研究。

Results suggested that Terminalia catappa leaves have no effect on cell viability of SCC-4 cells. The modified Boyden chamber assays revealed that a treatment of Terminalia catappa leaves significantly inhibited the cell motility/invasion capacities of SCC-4 cells.

在本实验中，利用 50%酒精萃取的榄仁树的叶之萃取物处理 SCC-4 细胞株，并利用 MTT assays 分析方法显示榄仁树的叶萃取物并不会影响 SCC-4 细胞的存活率，而当癌细胞转移时常常会伴随著细胞外基质的分解及细胞移动能力的改变，接著我们利用 modified Boyden chamber assay 发现榄仁树的叶萃取物具有抑制 SCC-4 细胞的移动与侵袭能力。

In addition, the repair method can also be filled tree holes, stuffing is best to use small gravel and cement mixture to be compacted filler, filler to strengthen the connection with the xylem, inside the cave can be a number of electroplating nails nails, holes dug in on both sides 4 centimeters deep groove at the bottom of filler from the start, each 20-25 cm for the layer, using linoleum separated from each floor should be inclined slightly outward to facilitate drainage, the edge should not exceed xylem, so that cambium in the formation of callus on it.

另外，也可用填充法修补树洞，填充物最好用小石砾和水泥的混合物，填充物必须压实，为加强填料与木质部的连接，洞内可钉若干电镀铁钉，洞口内两侧挖4厘米深的凹槽，填充物从底部开始，每20厘米至25厘米为一层，用油毡隔开，每层都要向外略斜，以利排水，边缘应不超过木质部，使形成层能在它上面形成愈伤组织。

It is considered that, just as those of pelagic sediments, the anomaloushelium isotope ratios of nodules can be attributed to micrometeorite interplanetary dust particles.

根据地外~3He的通量和结核、沉积物中地外~3He的含量计算了结核的生长速率和沉积物的沉积速率。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。