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A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,自9例慢性HBV感染患者血清中扩增HBV X基因,克隆入pGEM Teasy质粒,随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守,但每个序列均不一致。X区除了存在广泛的碱基点替换突变外,序列的缺失突变占测序克隆总数的51.4%(19/37);氨基酸缺失及移框突变多发生于123位氨基酸残基之后,可导致X蛋白多种羧基端形式。

The results show that: Basic density,tracheid length,tracheid width and the ratio of the tracheid length and width increase from core to bark; basic density has a small decrease from base to top,but the change is not significant;The tracheid length,tracheid width and the ratio of the tracheid length and width increase step by .step from the base up to certain height and then decrease by degrees.

基本密度自树干基部向上呈减小趋势,但变化不大;管胞长度、管胞宽度及管胞长宽比自树干基部向上呈&小—大—小&的变异规律;木材的化学成分含量和PH值除抽提物含量随着树高的增加而下降外,其它指标在树高方向上的变化未呈现明显规律性。

An annotation is any feature that can be tied to genomic sequence, such as an exon, transcript, promoter, or transposable element.

注释是可以束缚在基因组序列上的任何特征,例如外显子、转录物、启动子或转座元件。

The invention relates to a pipeline polyurea outer anticorrosive coating and the making method thereof, making heated prepolymerization reaction on dibenzo-methane diisocyanate, carbodiimide diisocyanate, polyether binary alcohol and polyether trihydric alcohol to prepare prepolymer solution A, and making end amino polyethylene oxide ether, dimethyl sulfur-based toluene diamine, diethyl diamine, iso-propyl titanate solution, polyacrylate, carbon black, titanium dioxide and lightweight calcium carbonate into solution B, and as using, respectively heating the solutions A and B to 65 deg.C and then mixing them, then quickly spraying and curing to form a coat.

本发明涉及一种管道用聚脲外防腐涂料及制造方法,由二苯基甲烷二异氰酸酯、碳化二亚胺化二异氰酸酯、聚醚二元醇和聚醚三元醇通过加热预聚反应制成预聚物的A液、由端氨基聚氧化乙烯醚、二甲硫基甲苯二胺、二乙基甲苯二胺、钛酸异丙酯溶液、聚丙烯酸酯溶液、碳黑、钛白粉和轻质碳酸钙配制而成的B液组成,使用时将A液和B液分别加热至65℃后混合,然后快速喷涂固化形成涂层。

Factors in VVC patients ,the VVC patients used of uncleaned ladies'goods is 9.46% and used of antibiotic is 16.11%, used of vulva wash is 17.39. Conculsion:1.The incidence of VVC is the hightest. The age of VVC is among 18 to 35.2.The symptoms of VVC are obrious vulva pruritus ,lightly pain in vulva and the congestion in 1/3 vagina mucositis. 3.There is obviouly difference in uncleaned ladies'goods and used of antibiotic and used of vulva wash.

1、妇产科门诊就诊的患者中VVC所占比例最高,且以18-35岁年龄为主;2、VVC患者主要症状及体征为外阴明显瘙痒、轻度阴道疼痛、阴道壁粘膜充血、阴道分泌物多且无溢出,以单纯性VVC较多见;3、经期使用不洁卫生用品、长期使用抗生素及经常使用阴道冲洗器有显著性差异是VVC的危险因素。

The dendrogram by UPGMA cluster analysis showed that 260 accessions of the 316 were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna unguiculata.

URGMA聚类图显示,13对SSR引物即能将其中的260份参试资源区分开,其中国内、外资源差异明显,被划为2大类群;国内资源类群又可分为与地理来源的气候生态区明显关联的2个北方组群、4个南方组群和2个混合组群,8个组群间相对独立又相互渗透;国内育种高代材料的遗传多样性狭窄。

It's suggested that the genes carrying DFR-1、OsMlo-1 might play a role in disease resistance of rice. Based on the putative exons of DFR-1 and OsMlo-1, primers were designed. The experiment had been performed by RT-PCR to detect the expression of gene carrying DFR-1、QsMlo-1 in rice uninoculated, inoculated with Xanthomanas oryzae pv.

为了进一步研究水稻DFR-1、OsMlo-1所在基因的功能,在DFR-1、OsMlo-1假定的外显子上设计引物,通过RT-PCR技术,研究在接种白叶枯病菌株PX099以及接种稻瘟病菌株V86013前后,水稻品种IRBB13和水稻品种明恢63中DFR-1、OsMlo-1所在基因的表达。

Except vanillic acid,there were no direct relationshipsbetween phenolic acids in roots and root exudates of soybean.

除了香草酸外,大豆根中的酚酸与分泌物中的酚酸无直接关系。

If the cause is loss of volume, intravenous fluids should be supplemented; if vasodilatation (f. ex. due to large doses of epidural analgesia or habitual anti-hypertensive medication), the treatment is not fluid but is the dose adjustment of the provoking factor or vasoconstricting agents as e.g., Ephedrine.

如果低血压由液体不足引起,应增加静脉补液量;如果血管扩张(例如,因为大剂量硬膜外麻醉或习惯性的抗高血压治疗),则不需液体治疗而是调整扩血管药物的剂量,或使用血管收缩剂,比如麻黄碱。

The secretion from vulval cancer can result in the change of the urine routine test as the urinary tract infection.

外阴癌分泌物可污染尿液,引起尿路感染样的小便常规检查结果。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。