外源物

In order to explore the mechanism of 'brumal jujube' of maturation and scenescence and ethanol accumulation, We not only studied the quality and the post-harvest physiological and biochemical changes of 'brumal jujube' after treatment with hormone during storage, but also investigated the effects of CAi (C027%~10%02+ 2~5%C02) and CA2:(7%~10%02, C02 2%) on the changes of fermentation volatiles, activity of enzymes , the kind and content of sugar and organic acid during storage in this paper. Firstly announced the reason of ethanol accumulation under higher C02 level and discussed the mechanism of ethanol accumulation during the end of storage.

本研究以冬枣为试材，采后采用激素进行处理，通过对贮藏期间果实品质、生理生化指标、果实衰老相关指标和酶活性的测定，系统探讨了外源激素处理对果实采后品质及衰老的调控作用；另外对点红冬枣采用CA_1：(7％～10％O_2+2～5％CO_2)和CA_2：(7％～10％O_2、CO_2≤2％)进行气调贮藏，通过测定冬枣果实品质、乙醇发酵相关酶活性及代谢产物、糖酵解底物及参与三羧酸循环代谢的有机酸含量和种类，研究了冬枣贮藏后期乙醇积累的机制，探讨了枣果不耐高浓度CO_2贮藏的原因。

Firstly, the recombinant engineering technology related vaccine preparation was set up, including: 1. the gene knock-out based on linear DNA target molecular in vivo; 2. the selection and counterselection technology of exogenous gene knock in definite site on chromosome; 3. the Gap-repair clone technology in vivo; 4. the recombination technology of single-chain DNA and the overlapping primer; 5. Set up the new selection and counterselection technology of neo-E.

本研究首先建立与疫苗制备相关的重组工程技术包括：①基于线型DNA打靶分子的大肠杆菌体内基因剔除技术，②选择与反选择外源基因定位敲入染色体上特定位点技术，③Gap-repair体内克隆技术，④单链DNA重组技术和重叠引物介导的DNA重组技术，⑤建立&抗生素-酶切位点&选择反选择技术新技术。

In view of vagina exists avariety of microbacteria, once ecological balance of vagina is destroyedor ectogenic pathogen break in, it canlead inflammation, cervix excretionincreasing and humidity verdict for pathogen produce, and endocervixplica is very much, focus of infection is difficulty eliminated. Becauseof the vagina and cervix that internal environment and anatomicalstructure is particular, the chronic cervicitis is always manifestrepeatedly, difficult to recover.

由于阴道内本身存在各种微生物，一旦阴道生态平衡打破或外源病原体入侵，即可导致炎症，宫颈分泌物多，局部潮湿，也有利于病原体生长，加之宫颈内膜皱襞较多，感染灶不易清除，慢性宫颈炎常常反复发作，缠绵难愈。

To modify the structures of erythromycin analogs in S. erythraea, stable expression vectors need to be constructed.

为了对红霉素类似物进行结构修饰研究，需要能够在糖多孢红霉菌中有效表达外源片段的载体。

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应，本文采用化学和分子生物学相结合的方法，研究了不同浓度乙烯利处理玉米后，叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化，典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化，以及其他调控次生代谢物的关键基因表达的时间动态。

The results of PCR and PCR-Southern blot assay indicated that the DREB1A gene was transferred into Lolium multiflorum Lam with a transformation frequency of 2.14％.

抗性植株的总DNA用DREB1A基因的特异引物进行PCR检测，转化频率为2.14％，PCR-Southern blot进一步验证了转化植株基因组中含有该外源基因。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

从GSSV中鉴定出超过25种蛋白组分，主要包括：酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖－2-氧化酶以及ablomin， catrin， mucrofirase， pallabin， salmosin和triflin的同源蛋白。

Bile and gastric and pancreatic secretions are re- sponsible for zinc excretion, and once in the intestine this endog- enous zinc behaves just like the dietary zinc.

胆汁、胃液和胰腺分泌物有排泄锌元素的功能，一旦吸收进入肠道，这些外源的锌元素和饮食中的锌有相同的表现。

Sorghum genome DNA had been transfered into rice variety via pollen tube pathway. In this paper, the molecular verification was performed by SSR technique. The result shown seventeen of sixty pairs of primers can reveal polymorphisms among donor, receptor and their stable offspring. Same bandings were amplified between donor and some offspring.

从60对SSR引物中筛选出17对，对供体高粱、受体水稻及其外源DNA导入后代稳定材料进行了分子验证，结果表明供体高粱与受体水稻间存在多态性、受体水稻与后代稳定材料间出现多态性，供体高粱与部分后代稳定材料间出现相同的扩增带，部分供体与受体共有的特征带在后代中消失、而有些后代出现了供体和受体均没有的扩增带。

The results showed that, NO-3-N contents and nitrate reductase activity had obvious diversity in different spinach varieties and different organs of the same plant. The NO-3-N contents in stem were the highest, followed by petioles, then mesophylls, and were the lowest in roots. When the ratio of root to shoot was higher, the NO-3-N contents in plants were lower. NR activity was higher in root especially in lateral root. The NO-3-N contents in mesophyll and petiole were negative correlated with the in vitro -NR activity and in vivo -NR activity in roots.

硝态氮含量以短缩茎中最高，叶和叶柄中次之，根中最低，且根/冠比越大的品种，其整体植株中的硝态氮含量越低；根尤其是侧根NR活性较高；叶肉和叶柄中硝态氮含量均与根中以内、外源硝酸盐为底物的NR活性呈一定的负相关。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。