外毒素的

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

RESULTS We successfully obtained human caspase-3 gene, the genes and expression vectors of reversed caspase-3 and its fusion protein with part of the translocation domain of Pseudomonas exotoxin A. Compared with the control group, the expression of both genes caused in a similar degree the decrease of living cell numbers and massive occurrence of apoptosis.

结果成功获得了caspase-3基因，构建了重构型caspase-3基因和重构型caspase-3与绿脓杆菌外毒素转膜结构域部分肽段的融合蛋白基因及其表达载体，与对照组相比，在转染后1～4 d内，两种基因的表达均导致HeLa细胞存活数减少，大量细胞发生凋亡，且二者活性相当。

In order to obtain the novel recombinant exotoxin fusion protein B7-PE40KDEL and to further explore the mechanisms of proliferation, differentration and related signaling transduction regulated by B7-1 and B7-2, their extracellular region cDNA were obtained via PCR.

为了研创B7-PE40KDEL新型重组外毒素融合蛋白，同时也为今后更深入地探讨B7-2和B7-1分子在调控T细胞增殖、分化及相关信号传导过程中的作用，本研究首先通过PCR克隆获得了含编码人B7-2和B7-1分子胞外区的cDNA。

The secretion of δ-hemolysin well reflects the regulation of access gene regulation system in S. aureus.

δ溶血素的分泌反映了金黄色葡萄球菌密度感应系统调控外毒素分泌的特点。

Enteroscopy was performed in 3 patients,and some typical pathological changes were discovered in sigmoid colon and rectum.

抗生素相关性肠炎是难辨梭状芽胞杆菌的外毒素所致的结肠和小肠粘膜的急性坏死炎症，并在坏死的粘膜上形成伪膜[1]。

An administrative core unit will be set up with missions of taking care of all the exter

综合而言，以这样一个高度整合的研究计画，从临床、实验及治疗的方向在病人、细菌、细胞层次、和分子层次作系统性的研究，探讨热原性外毒素B及其他致病因子的功能，将会有助於我们对於A群链球菌的致病作用进一步了解，更重要的是这些资讯将会提供临床治疗的新策略。

The biological effect of pUCP18/lasR was detected by RT-PCR, NAD method and the assay of pyocyanin.

RT-PCR检测LasB基因和LasI基因mRNA的表达，NAD法测定外毒素A的活性，紫外分光光度计测定绿脓菌素的产生水平。

the botulism toxin is the bacillus botulismus produces exotoxin, for present known toxin toxicity strongest.

肉毒毒素中毒致呼吸骤停，经心肺复苏抢救成功一例肉毒毒素是肉毒杆菌产生的外毒素，为目前已知毒素中毒性最强的。

Using immuno-histochemical, tissue cultural and immuno-electron microscopic methods, based on nerve-endocrine-immune net, the dynamic changes of 4 brain-gut peptides and 6 inflammation factors of excised saccalus rotunds of rabbit stimulated by germs, LPS, exotoxin were observed.

本研究以神经-内分泌-免疫网络学说为依据，运用免疫组织化学的方法，结合组织培养技术、免疫电镜技术等，对离体兔圆小囊受到大肠杆菌等4种细菌、内毒素、外毒素等抗原刺激后，其组织内的4种脑肠肽和6种炎症相关因子表达量的动态变化进行了系统观察研究。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。