外毒素

In this paper, we report expression and functional analysis of CK gene in amphioxus B. belcheri tsingtauense.

首先，我们用Real-time PCR技术分析细菌外毒素LPS对BbCK基因表达的影响。

Objective Study on superantigen Pseudomonas aeruginosa exotoxin A and its relationship with accessory cell.

目的 研究铜绿假单胞菌外毒素A（PE的某些抗原性及其与辅助细胞的关系。

But after stimulated by exotoxin, the content of NF in dome decreased significantly.

而受到外毒素刺激后，在圆小囊淋巴组织圆顶部位出现了显著降低。

Results When RPE cells were cultured with the exotoxin ATCC29213, the LDH level and necrotic RPE cells were positive proportional to the dosage of exotoxin, but only 250μl or 500μl of ATCC29213 had a statistical significant effect.

结果 金黄色葡萄球菌外毒素单独作用RPE细胞时，细胞外LDH水平及RPE细胞的坏死细胞比率随着外毒素剂量增加而升高，但仅250、500μl组差异有统计学意义。

Streptococcal pyrogenic exotoxin B is a cysteine protease and carried by every strain of Streptococcus pyogenes. SPE B has been shown to play an important role in invasive group A streptococcal infection.

链球菌致热性外毒素B 是一种cysteine protease，所有A群链球菌都携带致热性外毒素B之基因，在A群链球菌侵入性感染的病程中，致热性外毒素B扮演一个重要的致病角色。

Pseudomonas aeruginosa can produce a variety of toxin,and Pseudomonas aeruginosa exotoxin Ais a lethal exotoxin and the most important pathogenic factor.There is some research on the structure and mechanism of PEA.

绿脓杆菌能产生多种与毒力有关的物质，其中绿脓杆菌外毒素A(Pseudomonas aeruginosa exotoxin A，PEA)是一种致死性外毒素，是绿脓杆菌最主要的致病因子。

Streptococcus mitis exotoxin is a novel Streptococcus pyrogenic exotoxin.

缓症链球菌外毒素蛋白是一种新的链球菌致热性外毒素。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

These exotoxins are superantigens.

此菌会分泌许多外毒素，其中，以金黄色葡萄球菌肠毒素B为异位性皮肤炎患者皮肤上最常见的金黄色葡萄球菌外毒素。

This Program Project is composed of five projects and an administrative core unit. The specific aims of Project 1 are to study the roles of SPE B, SPE A, M protein and NADase to correlate with bacterial virulence and disease severity in different clinical spectra of GAS infections. We will also study the pathogenic factors of a unique virulent clone of Ml strain caused dominant invasiue GAS disease in Taiwan in the past two years. The effects of penicillin and clindamycin on the production of SPE A and SPE B will also be determined.

本群体计画包含有5个子计画及一个行政核心计画，第一子计画的目标在於研究热原性外毒素A，B和M蛋白及NADase在A群链球菌感染中的功能及其与细菌致病力、疾病严重性的相关性，同时将针对一株最近在台湾造成侵犯性较强的A群链球菌菌株研究其致病因子的异同，并将探讨抗生素penicillin及clindamycin对於热原性外毒素A及B产生的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。