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外显子

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Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应-单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26, Exon 30)及21内含子(Intron21)基因多态性,进行基因序分析,研究这些基因多态性是否存在连锁关系;应用Fok1酶切法对147例血液病人人类血小板抗原-3(human platelet antigen-3, HPA-3)基因进行分型,并与110例正常人进行比较;将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组,血小板输注3次以后检则血小板抗体。

Methods Using polymerase chain reaction-single strand conformation polymorphism, all the 41 exons of TSC2 gene were analyzed in 4 TSC cases (include 1 suspect case) from one family and 1 sporadic TSC case, and compared with the kin familial controls and kinless normal controls.

采用聚合酶链反应-单链构象多态性技术,对TSC一家系4例TSC患者(其中1例疑似)、1例散发性TSC患者外周血TSC2的41个外显子进行检测,并与家系中健康对照组和无血缘关系健康对照组进行比较。

The comparison between the GuHMGR1 genomic sequence with the cDNA of Pisum sativum showed that the genomic DNA contained two introns.The lengths of three exons were 1206 bp,181 bp and 943 bp,respectively.

GuHMGR1基因在DNA水平上由3个外显子和2个内含子组成;GuHMGR1的cDNA全长为2330bp,其中包括1518bp的开放阅读框、311bp的5'非翻译区和501bp的3'非翻译区。

Among its many biological activities, the major function of GM-CSF is to stimulate hematogenie stem cells prolification from silent stage to S phage, so that the stem cells can differentiate into granulocyte-macrophage cells.

有4个外显子和3个内含子,基因定位于5号染色体长臂5q21-5q32区,大多数器官均能产生GM-CSF,GM-CSF有多种生物活性,作用于多种细胞谱系,其主要作用是刺激造血祖细胞增殖即从静止期进入S期,并分化为粒细胞---单核巨噬细胞。

Results No mutations were found in GJA8 gene and GJA3 gene in all patients of this pulverulent cataract family.

结果 在该粉尘状白内障家系GJA8基因和GJA3基因的外显子及其邻近的内含子中,均未发现任何突变。

By the method of polymerase chain reaction-single strand conformation polymorphism, this paper studied the genetic polymorphism of exon 1 and intron 1 in the aquaporin gene of seven species of Stipa in the meadow steppe, typical steppe, and desert steppe of Inner Mongolia.

以分布于内蒙古的草甸草原、典型草原、荒漠草原上的7种针茅属植物为材料,利用聚合酶链反应-单链构象多态性方法,分析了水孔蛋白基因的外显子1和内含子1的遗传多态性。

Intercomparison.Sequence from Intronl to Exon2 fragment amplification by primers 2 of two individual selection from each swinery with pig L-FABP gene sequence published in GenBank,found 7 polymorphism loci existence in Individual.

引物2扩增的部分内含子1到外显子2片段每个猪群选择两个个体经测序得到的序列与GenBank上已发表的猪(DQ182323)的L-FABP基因序列比对,发现所测个体间存在7个多态位点。

An annotation is any feature that can be tied to genomic sequence, such as an exon, transcript, promoter, or transposable element.

注释是可以束缚在基因组序列上的任何特征,例如外显子、转录物、启动子或转座元件。

Results: P53 protein overexpression was showed in GBC-SD cell lines. A transversion of TAC→AAC at codon 126 of exon 5 was confirmed.

结果:GBC-SD细胞P53蛋白过表达;直接测序发现第5外显子126位密码子存在TAC→AAC的碱基突变。

Among the three transcripts, OsIM1 and OsIM1a share the same coding region and encode functional proteins, while the alternatively spliced OsIM2 are interrupted frequently by stop coden.

其中OsIM1与OsIM1a为编码基因,具有几乎完全一致的碱基序列,而OsIM2由于内含子、外显子剪切的错乱不具有编码功能。

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