外显子

CML is cytogenetically marked by the philadelphia chromosome, which originates from a reciprocal translocation between chromosome 9 and 22 and is molecularly marked a chimeric bcr-abl gene, resulting from juxta-positive of the abl proto-oncogene on chromosome 9 with the bcr gene, which is normally located on chromosome 22. The chimeric bcr-abl gene expression an 8. 5kb hybrid mRNA transcript giving rise to a 210-KD fusion protein (P210〓) with increased tyrosine kinase activity. P210〓 plays a key role in the pathogenesis of CML. The continuous cell line K562 was established from the pleural effusion of a 53-year-old female with CML in terminal blast crisis, and was a human erythroleukemia line, contained Ph chromosome.

绝大多数慢粒患者白血病细胞中具有Ph染色体，是由9号染色体长臂3区4带和22号染色体长臂1区1带相互易位形成，即t（9；22），使位于9q〓的c-abl原癌基因在第二外显子的5'端断裂并易位到22 q〓的M-bcr基因第2或第3外显子的3'端，形成异常的bcr-abl嵌合基因，该基因转导出异常的mRNA，编码并翻译出P210蛋白，该蛋白具有很强的酪氨酸激酶活性，使粒细胞发生恶性增殖。K562细胞属于慢粒急变、红白血病细胞株，具有Ph染色体。

In this study, the exon2 fragments of CHS genes in ferns, gymnosperms and angiosperms, a total of 22 fragments from 12 systematic families, were amplified successfully by PCR, and then sequenced (PCR amplification of the exon 2 from musci is in process).

本实验用PCR方法从蕨类，裸子植物和被子植物，共12个科中均成功地扩增了22个CHS基因外显子2片段（从藓类中扩增CHS基因外显子2的工作正在进行）。

To investigate distribution of gene mutations in LPL exon 9 in Chinese population and to study the possible effects of the mutation on plasma lipids, variants were screened by PCR-SSCP and DNA sequencing. Ser~(447)→stop polymorphism was identified by PCR-RFLP . the plasma lipids in stop447 carrier group and stop447 noncarrier group were compared and analysis.

为探讨中国人群LPL基因外显子9常见突变的种类、频率以及其可能产生的影响，我们利用聚合酶链反应-单链构象多态性分析和DNA测序技术及PCR-RFLP技术检测出我国人群的LPL外显子9基因突变、基因型和等位基因频率，确定了中国人群Ser~(447)→stop这一多态性位点。

PCR-SSCP method was used to detect mutations in exons 2,3,4 of runx3 gene in the gastric carcinoma.

结果：2例胃癌标本在runx3的第3外显子发现突变，第2、4外显子未发现突变改变。

In the process, some of the exons are retained while others are excluded.

在剪接过程中有些外显子保留下来有些外显子则不会。

Moreover, 13 ADHD specific haplotypes in contrast to worldwide population samples were identified by direct sequence of the exo 3 of DRD4 for 128 ADHD probands.

对DRD4基因外显子3和外显子4的序列测定表明在128个ADHD个体中发现了13个ADHD特有的单倍型序列。

Methods One hundred seventy-five coronary artery disease patients and 228 normal subjects were recruited and their blood samples were amplified to detect sequences of all 11 exons of MEF2A gene by PCR. Single-strand conformational polymorphism analysis was used to detect the mutation.

对175例冠状动脉疾病患者和228例正常对照的血标本进行PCR扩增MEF2A基因的11个外显子，然后采用SSCP方法检测外显子的突变并对扩增产物进行纯化和测序分析。

The follicle-stimulating hormone beta-subunit gene was studied as a candidate gene for the prolificacy in Jining Grey goats. According to the sequence of ovine FSHβ gene, nine pairs of primers were designed to detect single nucleotide polymorphisms of 5' regulatory region, exon 1 and exon 2 of FSHβ gene in both high fecundity breed and low fecundity breeds (Liaoning Cashmere goat, Boer goat and Angora goat) by PCR-SSCP.

采用PCR-SSCP技术检测促卵泡素β亚基(follicle-stimulating hormone β， FSHβ)基因5'调控区、外显子1和外显子2在高繁殖力山羊品种和低繁殖力山羊品种（辽宁绒山羊、波尔山羊、安哥拉山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。

Single nucleotide polymorphism in exon 1 and 3 of fibroblast growth factor (FGF5) gene was studied by DNA sequencing in Yingjing angora rabbit, Tianfu black rabbit and California rabbit.

通过PCR产物直接测序的方法，对荥经长毛兔、天府黑兔以及加利福尼亚兔的 FGF5基因外显子1和外显子3进行单核苷酸多态性分析。

A frameshift mutation at base position 217 of exon 1 and a T/C missense mutation at base position 59 of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 of exon 3 was found with similar frequency in three rabbit breeds.

在外显子1的217位检测到由TCT三碱基插入引起的移码突变，在外显子3的59位和3位分别发生了错义突变由T→C和同义突变由T→C。通过计算发现各位点不同的基因型和等位基因频率在3个兔品种中存在较大的差异，位点A、B在长毛兔和肉兔中均有各自的优势基因型和等位基因。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。