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Methods The polymorphism at nucleotide 307 in the 4th exon of HCR gene was detected in 118 patients of the Uigur population with psoriasis vulgaris and in 127 normal controls.

方法测定118例新疆维吾尔族寻常性银屑病及127例正常人对照组的HCR基因第4外显子第307位点基因多态性。

Using a pair of special primers to amplify the exon 3 of Myostatin gene in Northeast Wapiti, we aimed to detect the polymorphisms of Myostatin gene.

应用pcr-sscp和测序的方法对东北马鹿Myostatin基因的第3外显子进行了多态性分析。

Methods By real-time PCR, the damages of exon 5 and 8 of p53 gene were probed in 37 workers exposed highly to, 16 manager and logistic employees exposed less to an arsenic mill in Yunnan province, and also 25 local people who did not contact with any white arsenic in near past time. At the same time, the urinary total and organic arsenic of workers were detected.

选择云南某砒霜厂-线工人37例、管理和后勤人员16例和当地近期无毒物接触人员25例为研究对象,应用实时荧光定量PCR检测人群p53基因第5和第8外显子损伤,同时检测尿中总砷和有机砷含量,评价人群对砷的代谢转化与p53基因损伤的相关性。

In the phylogenetic tree, the cattle and zebu were clustered together first, followed by the yak, buffalo, pig, human, mouse, and rat.

HSL基因外显子Ⅰ部分核苷酸序列进行多序列对位排列构建的各物种间分子系统进化树结果表明,普通牛和瘤牛首先聚为一类,再分别与牦牛、水牛、猪、人聚类,最后与大鼠、小鼠聚为一类。

The partial sequences of exon Ⅰ of hormone-sensitive lipase genes in yak, cattle, zebu, and buffalo were analyzed. Comparisons of these sequences and the deduced amino acid sequences with the homologous HSL gene and protein sequences in other mammalian species including pig, human, mouse, and rat retrieved from the GenBank were carried out and finally a phylogenetic tree was constructed using the partial DNA sequences of the HSL genes in all species.

在对牛科中4种动物即牦牛、瘤牛、普通牛和水牛HSL基因外显子Ⅰ部分核苷酸序列进行测定的基础上,与Gen- Bank中其他物种相应基因核苷酸序列、氨基酸序列进行了比对分析,并构建了牦牛与其他物种间分子系统进化树。

Two other missense mutations (G288R and 711D) and a 1944-bp genomic deletion coding for exons 16 to 19 in the FLNA gene were identified in 3 additional, smaller, unrelated families affected by alular dystrophy, which demonstrates the responsibility of FLNA as a cause of XMD.

在另外3个患瓣膜营养不良症非该家族成员中,我们证实有两个编码外显子16和19的基因错义(G288R和711D)和一个基因缺失。这证明FLNA是XMD的致病重要原因。

Methods With polymerase chain reaction-single-strand conformation polymorphism combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism, the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.

通过聚合酶链反应-单链构象多态性分析结合银染后胶回收、DNA测序和限制性内切酶酶切分析方法检测112例明确冠心病诊断患者的ABCA1基因全部编码区50个外显子的SNP。

Three combinant retrovial expression vectors, aM1, aM2 and aM3, which was respectively loaded with reverse fragment of c-myc exon 1, 2 and 3, were transferred into rat arterious smooth muscle cells and assayed 1 month after their stable expression.

将分别载有c-myc第1、2和3外显子反义片段的三种重组逆转录病毒表达载体aM1、aM2和aM3导入大鼠主动脉平滑肌细胞,并于持续筛选后1个月进行稳定表达检测。

Methods Three recombinant retroviral expressive vecto rs, aMl, aM2 and aM3, which respectively loading reverse fragment of exon 1,2 and 3, were transferred in to rat arterious SMC and assayed 1 month after their stable expression.

分别载有c-myc 第1、2 和3外显子反义片段的3种重组逆转录病毒表达载体 aM1、aM2 和 aM3 被导入体外培养的大鼠主动脉平滑肌细胞,并于持续筛选后1个月进行稳定表达检测。

Methods Bisulphate sequencing was applied to analyse the mcthylation of the CpG islands in the SNRPN gene locus.

方法应用重硫酸盐测序法对一例临床高度疑似PWS的患者及其父母的15q11-q13印迹中心SNRPN基因alpha外显子的10个CpG位点的甲基化情况进行分析。

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