外显子

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法：将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体，应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核，再将注射后的受精卵移植至假孕鼠的输卵管。

Methods All the coding exons of TSC1 and TSC2 genes of these two patients, unaffected member in the two families, and 100 unrelated population-matched controls were amplified by polyme- rase chain reaction.

采用聚合酶链反应扩增结节性硬化症患者、患者家庭中的正常人及100例健康对照的TSC1 和TSC2基因的全部外显子，并进行DNA序列分析。

Results: No methylation was found at 5'CpG island of exon 1 of p16 gene in normal tissue specimens whereas hypermethylation was found in 3 specimens of rhabdomyosarcoma.

结果： 对照7例p16基因第1外显子5'CpG岛3个酶切位点无甲基化， 3例肉瘤组织中存在这3个酶切位点的甲基化现象。

It is a nonintegral ribosomal protein, binding to ribosome via RNA and in the same way as many translation factors.

外显子14可能编码一个出核信号，对它的剪接可影响FMRP在细胞内的定位。

Titin is recently known as the largest protein which exists in the stiated muscle sarcomere and is dynamic both in biomechanics properties and biochemical functions. Four possible disease-associated mutations located in three exons(3,14,49) of titin gene have been identified in Japanese DCM patients in 2002. But we did nothing about the creative titin and DCM.

目的：肌联蛋白是由单基因编码的最大蛋白，普遍存在于心肌和骨骼肌，被称为第三肌丝，具有复杂的生物力学性质和生物化学功能。2002年日本研究人员报道了肌联蛋白基因第3、14、49外显子的4个基因突变可能与扩张性心肌病发病相关，而我国没有相关的研究报道。

The sequences of Cufrk2 were used to design primers and a 426 bp fragment, FRK, encoded 139 amino acids, which possessed a sugar-binding domain with 58 amino acids was isolated from satsuma mandarin leaves by RT-PCR.

根据Cufrk2序列的外显子部分设计引物，通过RT-PCR从温州蜜柑幼叶中扩增到426bp的cDNA片段（编码139个氨基酸，其中包括58个氨基酸的糖结合域）。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

We analyzed the polymorphism of MHC class II B gene in 14 Chinese alligators sampled from three different areas: the wild population of Xuancheng in Anhui, the captive population of Changxing in Zhejiang and the captive population of Anhui Research Center for Chinese Alligator Reproduction. The gene fragment was amplified using a pair of specific primers designed from the MHC gene sequence of the Spectacled caiman. A total of thirty-four different sequence (15, 10 and 9, respectively) were detected in sampled Chinese alligators.

为了研究扬子鳄种群MHC基因的多态性，我们选取了安徽宣城野生扬子鳄种群、安徽省扬子鳄繁殖研究中心饲养种群和浙江长兴饲养种群三个亚种群的14条扬子鳄，利用一对特异性引物扩增出扬子鳄MHC II类B基因第三外显子的部分片段，并对其进行了克隆、测序，结果从这些扬子鳄样本中共获得34种不同序列，每个亚种群内分别检测到15，9和10个不同序列。

By examining 22 sputum samples 15 from confirmed lung cancer patients ,5 from clinic suggestible lung cancer patients,1 from...

结果 ：P53 外显子 6，7，8的 PCR-SSCP银染阳性率在肺癌组及癌疑组分别为 6/ 1 5 (40 %)和 2 / 5 (40 %)。

Results A novel polymorphism in the ABCA gene was found in two patients:M V which exists in exon7 of ABCA gene and it's cDNA location is A09G and converses amino acid from Me thionine to Valeric.

果M V是一个新发现的ABCA基因SNP，此位点在外显子7，cDNA位置为A09G，并导致位氨基酸由甲硫氨酸转变为缬氨酸。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。