外壁

It is thus clear that besides Octoxinol and Benzalkonii Chloridum have the spermicidal and virucidal functions, the main function of the other elements is only to lubricate, make them become semisolid, clear gel and extend the periods of time in the vaginal walls.

可见，除辛苯聚醇和苯扎氯胺具有杀灭精子和病毒的功效外，其他成份的主要作用仅是润滑、使其成为半固态清晰的凝胶并延长在阴道壁的停留时间。

Methods insert the stomach tube nasal cavity, with the help of intubating ferceps, take the stomach tube out of mouth cavity from the posterior wall of dysphagia, insert trachea catheter (cid 7.0~7.5) to esophagus, under the help of trachea catheter, insert the stomach tube to esophagus then take the trachea catheter out of the esophagus and vertical, cutting off, in the course of into esophagus.

经鼻腔插入胃管，用插管钳将胃管从咽后壁前取出口腔外，用id 7.0～7.5的气管导管插入食管，在气管导管诱导下将胃管经气管导管插入食管，然后将胃管向食管内送进，同时将气管导管退出，将胃管置入相当合适的深度后，固定胃管。

Blood samples were taken for the measurement of RBC, Rb, Hct, plasma EPO concentration, whole blood/plasma viscosities, the animals were then catheterized to record mean pulmonary arterial pressure and demised to calculate the ratioCRV/D. Percentage of vascular wall thickness and muscularization of non-muscular pulmonary arteriole were examined microscopically.

取血样测定红细胞数、血红蛋白、红细胞压积、血浆EPO浓度、全血／血浆翻度，颈外静脉插管测定平均肺动脉压力；动物处死后计算右心室／〔RV/〕评价右室肥厚程度；光镜观察肺小动脉管壁厚度百分比、肺非肌性小动脉肌化程度。

Deep research and comparison show that, besides the centrarch xylem, H. reflexa has G-type tracheids. Upon and between the annular secondary thickenings, the lignified layer of secondary cell wall bears irregular simple perforations of small diameter and high density. So these perforations have the characters of S-type tracheids. The robust xylem strand and dense perforations indicate the strengthened water-conducting capacity.

深入的研究和比较表明H.reflexa的木质部除为心始式外，管胞为G-型，次生壁的木质化层具有不规则的简单穿孔，穿孔直径小，密度大，分布在环纹次生加厚带和其间，兼有S-型管胞穿孔的特征；粗壮的木质部束和密集的穿孔表明较强的输导能力。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Methods: The anterior walls of spheroid sinus were observed and measured (layout, position, external diameter, distances to other structures of interest) with a vernier caliper and an angle gauge on 15 adult cadavers.

在15例国人头颅标本上，用游标卡尺和角规对30侧蝶窦前壁进行解剖学（走行、位置、外径以及与相关结构的距离）观察和测量。

The top wall of the outer channel of the rail shll be 0.100" thick; the sidewalls shall be 0.120" thick for superior vertical load strength.

顶层墙体外通道的铁路shll成为0.100 &厚的侧壁应0.120 &厚的上级垂直载荷强度。

As the tumor spreads outward it can invade the chest wall and ribs, and this can be extremely painful.

由于肿瘤可向外蔓延侵入胸壁和肋骨，这可以十分痛苦。

At the same time, two different coal feeding methods - coal from the vertical pipe and from the inserted tube, were separately simulated and analyzed. According to the internal flow distributions under two different coal feeding methods, we made a judgement that the former feeding method was better than the latter.

为了进一步对煤粉捏合器进行优化设计，按照上述方法本文从改变旋转轴的转速、改变煤粉跟溶剂油的进口流量、改变搅拌桨叶形式以及改变搅拌桨叶外端与混合槽壁间距几个方面重新进行了数值模拟与分析。

The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid.

取小鼠外周血非贴壁的淋巴细胞，与靶细胞混合淋巴细胞培养，进行淋巴细胞增殖和反应性T细胞功能检测，并检测细胞培养液中IL-2、TNF-α和IFN-γ水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。