外区

The human CTLA-4 gene, which lies on 2q33, has four exons: exon 1 encodes leader sequence, exon2 encodes 116 Aa forming extra-cellular V-like functional domain, exon 3 encodes 37 Aa of hydrophobic membrane domain and exon 4 encodes 34 Aa of cytoplast domain.

人类的CTLA-4基因位于2q33，有四个外显子组成，其中第一外显子编码前导序列，第二外显子编码116个氨基酸组成的胞外功能区，第三外显子编码37个氨基酸组成的疏水性跨膜区，第四外显子编码34个氨基酸组成的胞内区。

Climatic gradients were based on data sets from 841 meteorological stations throughout China. Definitions for floristic distributional patterns were based upon the work of Prof. Z. Y. Wu. Most of these floristic distributional patterns were strongly correlated with the geographic gradients in climate, except for cosmopolitan, cultivated and invasive genera. Tropical genera form a large fraction of the total genera (ranging from 0.84% to 94.38% in the regional floras) with the highest proportion in southern Yunnan and Hainan Island. Tropical genera occur predominantly in southern China of 0° N latitude and decreased with increasing latitude, as would be expected. Interestingly, the disjunct Tropical Asia and Tropical America distribution were not restricted to southern latitudes. Temperate genera account for 5.1% to 98.83% of the total genera in regional floras with the highest proportion in the province of Xinjiang. Most of these genera followed geographic gradients in climate as expected (temperate genera conspicuously dominate the landscape at higher latitudes), except the East Asian and North American disjunct distribution, Eastern Asian distribution and Chinese endemic distribution. Generally, most plant genera demonstrated some correlation with climatic and geographic gradients. The most important gradients were those of annual air temperature and precipitation. A small fraction did not demonstrate significantly particular pattern:"Cosmopolitan","East Asian and North American disjunct","Eastern Asian" and "Chinese endemic" distributions. The North Temperate distribution had the highest correlation with mean annual air temperature and precipitation.

结果如下：(1)除世界分布、栽培和入侵成分外，大部分中国种子植物属的地理成分的分布与地理相关密切；(2)热带分布属（泛热带分布、热带亚洲至热带美洲间断分布、旧世界热带分布、热带亚洲至热带大洋洲分布、热带亚洲至热带非洲分布及热带亚洲分布合计）占各地方植物区系的0.84%到94.38%，其最低值出现在中国西北部的新疆和青海地区，最高值出现在中国云南南部和海南；(3)热带分布属在＜北纬30°的地区占优势，除热带亚洲至热带美洲间断分布外，其它热带成分随纬度增加迅速减少；(4)温带分布属（北温带分布、东亚和北美间断分布、旧世界温带分布、温带亚洲分布、地中海区、西亚到中亚分布、中亚分布和东亚分布合计）占各地方植物区系的5.1%至98.83%，其最高值出现在中国西北部的新疆地区，最低值出现在中国云南南部和海南；(5)除东亚和北美间断分布、东亚分布和中国特有分布外，其它温带成分随纬度增加迅速增加；(6)在温带成分中，东亚和北美间断分布及东亚分布属主要出现在中国亚热带到暖温带地区，北温带分布、旧世界温带分布和温带亚洲分布属在中国北部占优势，而地中海区、西亚到中亚分布和中亚分布属则在中国西北部占优势；(7)除世界分布、东亚和北美间断分布、东亚分布和中国特有分布外，所有其他成分都显示了与气候因素密切相关，其中，北温带分布属与年均温和年降雨量最为密切相关。

RESULTS:①There was higher tensile stress at the ingual side of symphysis, inside of molar and outside of condyle under the impact of chin.②There was higher tensile stress at the lingual side of symphysis, inside of contralateral molar, outside of contralateral angle, outside of homolateral angle and inside of homolateral body under the impact of body.③There was higher tensile stress at the inside of angle, inside of homolateral condyle, lingual side of symphysis, outside of contralateral condyle and inside of homolateral molar under the impact of angle.

结果：①撞击颏部，其舌侧区，磨牙后区内侧面、髁状突颈外表面以及下颌角外表面具有较高的张应力；②撞击下颌体部，颏部舌侧区，对侧磨牙后区内侧面、对侧下颌角外表面、撞击侧下颌角外侧面、撞击侧体部内侧面具有较高的张应力；③撞击下颌角，其内侧面、撞击侧髁状突颈内侧面、颏部舌侧面、对侧髁状突颈外表面以及撞击侧磨牙后区内侧面具有较高的张应力。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果：通过对数据的处理和分析，我们初步发现：1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域，小脑左侧更明显，左侧颞叶皮层激活点多于右侧，且脑部左侧深层激活点多于右侧；2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域，激活点主要集中表现在大脑皮层，以颞叶为主，小脑及深部未发现明显激活点；3、非穴真针刺组结果分析初步表明，BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点，大脑左、右侧均有激活点，但右侧更多，双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点，激活点主要集中在大脑皮层，深部激活点主要在壳核；4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点，主要分布在大脑的右侧，左侧也有不少激活点，就其具体分布主要在颞叶和额叶，少部分分布在顶叶和枕叶；5、外关穴真针刺与假针刺对比发现，BA8区和小脑等区域有明显的激活点；6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点；7、外关穴假针刺与非穴假针刺对比发现，BA7、8、9、18等区有激活点，主要反应点在左侧大脑的枕叶中回、楔叶，顶叶楔前叶及额上回、中回，就其分布不难看出主要在大脑中部，颞叶未见明显激活点。

Japan to time Tainan prefectural city Nanmenwai area, because under Japanese government's government order, starts large-scale changing burial sites, because changes burial sites namely the Taina metropolis plan which implements for the Japanese government, divides into three times, the seed time, the development time, launches the time, gradual expands Taina's metropolis scope, therefore in obtains the land in the process, an item of suburb generation of development, the Tainan prefectural city Nanmenwai's grave area becomes the date to the time Taina southing development center of gravity, after undergoing the grave migration the land, passes through plans, traveling schedule education, movement facility special-purpose, Japan to the time Tainan prefectural city Nanmenwai's grave picture, is penetratingUnder Japanese government's political power's changing burial sites, after as well as changes burial sites the construction, transforms the school and the athletic field, vital region.

日至时期的台南府城南门外地区，因为在日本政府的政令之下，开始大规模的迁葬，迁葬之因即为日本政府所实施的台南市都市计画，分为三个时期，萌芽期、开拓期、展开期，逐步的扩张台南市的都市范围，因此在取得土地的过程中，项城郊一代发展，台南府城南门外的墓葬区成为日至时期台南市南向发展的重心，经过坟墓迁移之后的土地，经过重新规划，行程教育、运动设施的专用地，日至时期台南府城南门外的墓葬景象，在透过日本政府的政治力量的迁葬之下，以及迁葬之后的建设，转变成学校与运动场，充满活力的区域。战争期间，因为战争需要，在台南府城南门外墓葬区的南侧建立台南军机场，因此在战争期间，台南府城南门外地区又带入军事的色彩，战后国民政府接收日本人撤退之后的所有建设，将空军后勤补给对进驻台南军机场，随之而来的眷属则占据日本人的宿舍，另外在新建多处的眷村，战后初期的眷村文化即进入台南府城南门外地区。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Only the LASS2 protein carboxyl terminal hydrophilic fragment could be expressed in the prokaryote expression system and its polyclonal antibody was produced.

在原核表达系统中仅能够表达其羧基端胞外区片段却不能表达完整的LASS2蛋白，并制备了该片段的抗体。

Conditioned area with two-pipe water system is divided into inner zone and peripheral zone.

系统分内、外区，水系统为两管式。

In his final play as a Major Leaguer, Ryan Church popped up to him, as a catcher, in foul territory.

Zeile最后一次的大联盟守备机会则是以捕手身分在界外区接杀了Ryan Church的小飞球。

The playing area is divided into the infield and the outfield. Together, these two areas make up fair territory. The rest of the field is called foul territory.

界内地区分内场和外场，这两个场地组成了界内地区，其余的地方为界外区。

This brought fixed cost, but it also is meant at the same time use a source to won't make you singlehanded assume a problem.

这带来了一定的成本，但它同时也意味着使用开源不会让你独力承担问题。

He gained a small fortune in real estate.

他在房地产上发了一笔小财。