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Because the unavailability of the three-dimensional structure for the wild-type, we have constructed a 3D model of the gp41 ectodomain trimer by using the homology method.

由于缺少gp41的三级结构,我们用同源模建方法构建了它的膜外区,用PROCHECK程序检验表明,模建的结构是合理的。

The azimuthally linear advection may results in that the meso-scale vortex of initially circular shape becomes the elongated and spiral vorticity bands; the radial linear advection may transfer the vorticity of the outer region towards the center of the typhoon.

沿e方向的线性平流可使初始圆形的中尺度涡旋演变为狭长的螺旋状的涡量带;沿径向线性平流的引进可使外区涡量向台风中心方向传播。

The immunohistochemistry method was used to detect the distribution and location of ephrin-A5, and the change of expression was detected at 1 week, 2 weeks and 4 weeks after operation.RESULTS: The results of immunohistochemistry showed that ephrin-A5 distributed in the region of V2MM, V2ML, V2L, Au1, AuV in damaged cerebral cortex, and located in the body of neuron, cytodendrite, and somewhat in axon.

结果: 免疫组化结果显示:大脑中动脉皮层支梗塞后ephrin-A5主要表达于梗死灶侧皮层的V2MM、V2ML(第二视皮质内侧外区、V2L、Au1、AuV,分布在神经元胞体、树突中,轴突亦有少量分布。

Human erythropoietin receptor plays an important role in regulating the red blood cell production by promoting the proliferation and differentiation of RBC from erythroid precursors.

以人胎肝为材料,通过RT PCR的方法扩增出人促红细胞生成素受体的胞外区基因。

In short, the extracellular region of small impact on consumer goods, but it can not be overlooked.

总之,外区货对消费者影响小,但也不能忽视。

Here, we show that mutations in GPR56, which encodes an orphan G protein–coupled receptor with a large extracellular domain, cause a human brain cortical malformation called bilateral frontoparietal polymicrogyria.

作者发现编码一个带有较大胞外区的孤儿G蛋白耦联受体的基因GPR56的突变导致人类大脑皮质畸形(双侧额顶多小脑回畸形BFPP)。

The preliminary results showed that the extracellular domain of YWK- II protein was able to interact with the 303-376 aminoacids segment of the rat Mullerian inhibiting substance in a yeast two-hybridsystem.

我们利用分子生物学的方法对YWK-Ⅱ蛋白质的463位至674氨基酸及389位至574位氨基酸的两段肽段分别进行了表达、纯化、结晶和初步解析了其中一段胞外区的结构,并且得到了YWK-Ⅱ和MIS的复合物的晶体。

High-level expression of secreted Flt-1(2-3) with good bi ological activity were successfully achieved in Pichia.pastoris expression syst em and can be applied to further animal and clinical test.

获得了具有生物学活性的可溶性重组F lt-1受体胞外区2-3loop小片段,为进一步的动物实验和临床应用打下了基础。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白,主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运,并维持一定细胞内外的离子梯度,我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增,限制性酶切分析扩增产物,并进行荧光测序,对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析,发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg,Fc。

The 20 outer boroughs are Waltham Forest, Redbridge, Havering, Barking and Dagenham, Newham, Bexley, Bromley, Croydon, Sutton, Merton, Kingston upon Thames, Richmond upon Thames, Hounslow, Hillingdon, Ealing, Brent, Harrow, Barnet, Haringey, and Enfield.

20外区的沃尔瑟姆森林, Redbridge , Havering ,门口和Dagenham ,纽汉,贝克斯利,布罗姆利,克罗伊登,萨顿,默顿,金士顿对泰晤士,里士满泰晤士河畔, Hounslow , Hillingdon ,伊灵,布伦特,哈罗,巴尼特, Haringey ,和恩菲尔德。

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This brought fixed cost, but it also is meant at the same time use a source to won't make you singlehanded assume a problem.

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