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外-斐反应

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Experimental data showed that methyl scopolamine and pyridostigmine could interfere with the thermoregulatory response to open field exposure,and indicated that peripheral cholinergic nerve involved in the control of stress hyperthermic response in the rat.

实验结果表明,吡啶斯的明和甲基东莨菪碱可以影响暴露在开放环境中大鼠的体温调节反应,提示外周胆碱能神经参与大鼠应激性体温过高反应的调节。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变,均可检测到克隆性增殖T细胞,尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖),对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点,并存在克隆性增殖的T细胞,这可能是机体对白血病相关抗原产生的特异性免疫反应;Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显,可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性,是构建DNA疫苗的基础。

The results showed that in addition to ACTH_(1-39)-immunoreactive neurons found in the infundibular nucleus of the hypothalamus which were identical with the previous reports, negafivt ACTH_(1-39)immunoreactive neurons were also found in paraventricular and supraoptic nuclei.

结果表明除了在下丘脑漏斗核观察到的ACTH_(1-39)免疫反应神经元胞体和以往文献报道一致外,在室旁核、视上核内也有ACTH_(1-39)免疫反应细胞体。

Methods Using polymerase chain reaction-single strand conformation polymorphism, all the 41 exons of TSC2 gene were analyzed in 4 TSC cases (include 1 suspect case) from one family and 1 sporadic TSC case, and compared with the kin familial controls and kinless normal controls.

采用聚合酶链反应-单链构象多态性技术,对TSC一家系4例TSC患者(其中1例疑似)、1例散发性TSC患者外周血TSC2的41个外显子进行检测,并与家系中健康对照组和无血缘关系健康对照组进行比较。

To investigate distribution of gene mutations in LPL exon 9 in Chinese population and to study the possible effects of the mutation on plasma lipids, variants were screened by PCR-SSCP and DNA sequencing. Ser~(447)→stop polymorphism was identified by PCR-RFLP . the plasma lipids in stop447 carrier group and stop447 noncarrier group were compared and analysis.

为探讨中国人群LPL基因外显子9常见突变的种类、频率以及其可能产生的影响,我们利用聚合酶链反应-单链构象多态性分析和DNA测序技术及PCR-RFLP技术检测出我国人群的LPL外显子9基因突变、基因型和等位基因频率,确定了中国人群Ser~(447)→stop这一多态性位点。

The endotoxin also named as lipopolysaccharide in the outer membrane of Gram-negative bacteria is the primary constituent of pathopoiesis. LPS which has extensive biological activity can induce severe inflammation in cascade and lead to sepsis、systemic inflammatory response syndromes、multiple system organ failure.

细菌壁外膜的内毒素又称脂多糖(lipopolysaccharide,LPS)是其主要致病成分,具有广泛的生物活性,能引起严重的炎症级联反应,是导致脓毒症、系统性炎症反应综合征、多脏器功能衰竭的主要致病因子。

Levels of expression significantly increased after day 4 posttransplantation and persisted through the whole course of rejection, but levels of expression were obviously lower in the peripherial blood than those in portal vein blood. In contrast, the control group showed no expression of mRNA for perforin and granzyme B in portal vein and peripherial blood.

结果 急性排斥肝移植组术后3天门静脉和外周血中均有穿孔素和颗粒酶B基因mRNA表达,4天后表达明显增强,并贯穿整个排斥反应的全过程,但外周血表达的强度明显较门静脉血为弱,同基因对照组术后14天内无穿孔素和颗粒酶B基因表达。

Results The rejection group began to show the mRNA of perforin and granzyme B up-regulation on day 3 posttransplantation, when no histologic evidence of rejection was found. Levels of expression significantly increased after day 4 posttransplantation and persisted through the whole course of rejection, but levels of expression were obviously lower in the peripherial blood than those in portal vein blood. In contrast, the control group showed no expression of mRNA for perforin and granzyme B in portal vein and peripherial blood.

结果 急性排斥肝移植组术后3天门静脉和外周血中均有穿孔素和颗粒酶B基因mRNA表达,4天后表达明显增强,并贯穿整个排斥反应的全过程,但外周血表达的强度明显较门静脉血为弱,同基因对照组术后14天内无穿孔素和颗粒酶B基因表达。

The immunohistochemical location of noncollagenous protein, bone sialoprotein , osteopontin and osteocalcin at different stages following periradicular surgery was undertaken. The crystals formation on surfaces of MTA was evaluated when treated in different conditions without cells in vitro and in vivo. MTA was used to manage clinical problems, including perforations in roots or furcations, and pulpless permanent teeth with incomplete root formation. The study included the following three parts

本研究试图通过体内外研究探讨MTA表面牙骨质形成的机理,动物体内动态观察根尖周组织在用MTA倒充填后的组织反应和细胞外基质非胶原蛋白的表达,体外观察MTA在不同的环境下表面的形态及成分变化,和在体内环境下材料表面磷灰石层的形成,同时结合临床病例观察其临床治疗效果。

The explants which showed vitrification could regerminate normal tepals when transfered to the medium supplemented with 6-BA 0.2 mg/L and NAA 0.005 mg/L, which demonstrates that decreasing the concentration of exogenous hormones could inhibit the vitrification.

玻璃化反应的外植体转移至MS附加6-BA 0.2 mg/L、NAA 0.005 mg/L的培养基上继续培养30 d后可再生正常的花被片,表明降低培养基中外源激素浓度能够阻止玻璃化反应继续发生。3。

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