外-斐反应

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Methods 80 cases were divided into control group and test group with 40 cases respectively. Epidural catheter was left in test group, connected with "Tuoren" patient-controlled epidural analgetic pump with dicaine 100 mg+morphine 10～15 mg+droperidol 5 mg,which were dissolved into NS to 100 ml. LCP model was as follows:morphine 2～3 mg, background dosage: 2 ml/h, PCA 0.5 ml each time; The control group injected morphine 3 mg through epidural conduit each time to relieve spasm and pain according to the symptoms to observe and record the effects and side effects. Results Test group:39 cases were with remarkable effect, 1 cases was common.

将80例前列腺术后随机分为试验组与对照组各40例，试验组术毕保留硬膜外导管，接国产&驼人牌&一次性患者自控硬膜外镇痛泵，药液配方为丁卡因100 mg+吗啡10～15 mg+氟哌啶5 mg加入生理盐水至100 ml，LCP模式（负荷量：吗啡2～3 mg，背景量：2 ml/h，PCA 0.5 ml/次）；对照组据疼痛症状经硬膜导管推注吗啡3 mg/次解痉止痛，观察并记录术后解痉镇痛效果及不良反应。

Transmembrane action potentials were recorded simultaneously from endocardium, epicardium, together with a transmural ECG, in arterially perfused left ventricular preparations by use of 2 separate intracellular floating microelectrodes. The stimulus-response- interval of the epicardium and the incidence of ventricular tachycardia were observed as well.

灌流全程同时采用浮置玻璃微电极法同步记录内膜下心肌、外膜下心肌跨膜动作电位和跨壁ECG，并观察心外膜下心肌的刺激反应间期(Stimulus-response-interval， SRI)和室性心动过速的诱发率。

Objective To investigate the activation of extracellular signal-regulated kinase and cAMP-responsive element binding protein in the anterior cingulate cortex induced by peripheral injection of formalin.

目的研究外周福尔马林刺激诱导的大鼠前扣带皮层中细胞外信号调节激酶和cAMP反应元件结合蛋白的激活。

Methods Eight coding exons of ED1 gene of two patients with clinically confirmed X-linked hypohidrotic ectodermal dysplasia, their parents, and 100 unrelated population-matched control were amplified by polymerase chain reaction. The products were further analyzed by direct sequencing.

采用聚合酶链反应扩增该家系中两名临床诊断为X性连锁少汗性外胚叶发育不良的患者、患者父母以及100例健康对照者ED1基因的8个外显子，进行DNA直接测序。

The mechanism of outside cell reaction of electrosynthesis of p-methoxybenzaldehyde and p-bromobenzaldehye is studied by Fourier-transform infrared absorption spectra.

利用红外光谱研究了槽外合成对甲氧基苯甲醛和对溴苯甲醛的反应机理，结果证明：在Ce4+与对取代甲苯的氧化反应中，苯环上甲基被Ce4+氧化的难易受苯环上对位取代基的影响。

At the BCL of 500ms, 1000ms and 2000ms,the VWtimes increased markedly with the BCL prolonging, and the degree of increasing was even obviously when heart rate was slow (BCL=2000ms or 1000ms). The enlargement of VWs was a most important reason to increase the incidence of ventricular arrhythmias; 2）At the BCL of 500ms, 1000ms or 2000ms,the VWtimes of the premature stimulates S2 was at endomembrane were wider than at the outermembrane. We supposed that premature stimulates come from endomembrane was easier inducing VF compared with outermembrane;3The VWtime of unidirectional block was wider than reentry's, we can see that reporlarization dispersion changed dynamicly. 4The QRS polar of the premature stimulates was opposite the primary beats ,which suggesting that excitation of inducing premature beats was coming from epicardial. That is to say electrotonic potential take part in the creation of reentry;5With the ratio of the Tp-e/QT increasing the occurrence of VF was upgrading. So the ratio was an effective method to estimate the event of SCD.6 The VWtimes of S3 were broaden than S2's. But we can not study penetratingly, which is the contents of our future studies.

在BCL为500ms、 1000ms和2000ms刺激条件下，随着刺激周长的增加单向传导阻滞时间易损窗呈增大趋势；并且在心率较慢时(BCL为2000ms和1000ms)增大最明显，是LQTS患者室性心律失常发生率增加的原因之一；2）在BCL为500ms、 1000ms和2000ms刺激条件下，S2位于内膜侧时时间易损窗大于在外膜侧时的，推测来自于内膜侧的期前刺激易于诱发室性心律失常；3）折返激动时间易损窗小于在单向传导阻滞时间易损窗，折返易发生于S2刚进入单向传导阻滞区间时，这说明复极梯度的不均一是动态变化的；4心电图上倒置QRS波与正常S1刺激所引起QRS波极性相反，提示不同位置S2刺激引发的室性早搏的兴奋均来自于外膜侧，说明了电紧张电位扩布参与了折返的产生；5）Tp－e/QT可以反应跨膜复极离散度，随着Tp－e/QT比值的增加PVT的发生增多，其可以用来预测室性心律失常的发生。6）随着期前刺激数目的增加时间易损窗增大。

When the investigators saw him at age four years, they found him on physical examination to be normal except for having two to three cm of dry, erythematous patches on both cheeks, as well as "mildly dry" skin on the flexor/extensor surfaces of both arms. They subjected him to a standard battery of tests and found negative results on skin prick testing to milk, eggs, peanuts, and dust mites, and serum IgE testing was also negative.

在这位男童四岁时，研究人员对他进行诊疗，在理学检查时发现，除了双颊2到3公分的乾躁、红肿斑块，以及双手伸肌或是屈肌的表面轻微乾躁外，大部分都是正常的；研究人员请这位男孩接受标准电池测试，结果发现皮肤刮痕试验结果对牛奶、鸡蛋、花生与尘蹒没有过敏反应，而且血清IgE试验呈阴性反应。

METHODS: Adults with chronic persistent cough (n = 25) and healthy controls (n = 11) were assessed by cough-specific quality of life questionnaire, extrathoracic airway hyperresponsiveness to hypertonic saline provocation, capsaicin cough reflex hypersensitivity, and fibreoptic laryngoscopy to assess PVCM.

对25名慢性持续咳嗽患者和11名健康志愿者进行咳嗽特异性生活质量问卷调查，并检测高渗盐溶液激发的胸腔外气道高反应性、辣椒素咳嗽反射超敏反应和纤维支气管镜来评价PVCM。

Extracellular single unit recordings in vivo were performed in the DG of learner animals during the consolidation of trace eyeblink CRs.

采用在体单细胞外记录技术，研究习得条件反应豚鼠的齿状回颗粒细胞在条件反应巩固过程中的放电活动。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了