外-斐反应

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The splicing reaction involves two transesterification steps which ligate the exons together and release the intron as a branched lariat structure containing a 2'5' bond with a conserved A residue in the branchpoint sequence. The splicing reactions require snRNPs and accessory proteins that assemble into a splicesome at the intron to be removed.

剪接反应包括两步转酯反应：外显子被连接在一起和内含子以分支的套索结构被释放，此结构含有一个与分支点序列中的保守A残基形成的2'5'键。

A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥，放入玻璃管内，密封得搅拌磁子；配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液，加入有机碱得预键合溶液，将搅拌磁子浸入预键合溶液，在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质；将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中，将预键合后的搅拌磁子放入容器，密封后置于水浴中，反应后除去容器，取出涂有聚合物的搅拌磁子，置于提取器中，以溶剂提取，或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出，得产物。

Methods We adopted four methods that single cpidural block anesthesia or narcotics analgesies were injected into subarachnoid space and epidural space or single narcotics was injected into subarachnoid space (group C fentanly 25μg;group D fentanly 20μg) to study analgesia birth for 130 nullipara women. The analgesia efficacy, side effect, birth time md mode,postpartum haemorrhage and the condition of the fetus and the newborn were compared.

采用单纯硬膜外腔阻滞、蛛网膜下腔-硬膜外腔联合给予低浓度麻醉药及镇痛药、单纯蛛网膜下腔给予低浓度镇痛药(C组芬太尼25μg，D组芬太尼20μg)等四种不同的方法对130例初产妇进行分娩镇痛，比较几组的镇痛效果、不良反应及对产程、分娩方式、产后出血、胎儿及新生儿等的影响，并与40例正常未干预组产妇比较。

No IL-6 levels were detectable at Day 0 in perilymph.The earliest perilymph IL-6 levels were observed after 6 h,peaking at 24 h, and then decreasing gradually.

结果：在发生继发性内耳反应初始，外淋巴液未检测到IL-6，而6 h后外淋巴液中检测到了IL-6的最早水平，24 h后IL-6水平达到最高峰。

We investigated the incidence of perception of and spontaneous verbal and motor responses to insertion of a spinal needle through the dura mater and pia mater and the effect of injecting lidocaine into the epidural space through the epidural needle before inserting the spinal needle through the meninges.

我们调查在穿刺针通过硬脊膜及软脊膜时不适感和不自主的口头及身体反应的发生率，同时调查在置腰麻针前硬膜外注射利多卡因对这些反应的影响。

Third, the results also revealed that the toddlers'rhythmicity, intensity of reaction, quality of mood and the parenting style of punishment were always lead to the incidence of the deregulation incidence of social-emotional development. In the end, the binary logistic result told us that toddlers'gender, age, apporoach and withdrawal, persistence, threshold of responsiveness, the parenting of refused, encourage independence and explore and low-voltage-induced all can lead to the incidence of the competence.(2) The degree of effect caused by genetic and environmental factors on the toddlers'social - emotional development were revealed by the structural equation model.

研究结果1。两分类logistic回归分析发现，影响中国幼儿情绪社会性外显问题的生物遗传及环境因素有年龄、性别、活动水平、趋避性及适应性，母亲文化程度、拒绝及惩罚的家庭教养方式；影响内隐问题的遗传及环境因素有年龄、活动水平、趋避性、情绪本质及拒绝、关注与保护、控制施压的家庭教养方式；影响失调问题的遗传及环境因素有节律性、反应强度、情绪本质及惩罚的教养方式；影响能力问题的遗传及环境因素有性别、趋避性、坚持性、反应阈、家庭收入、接纳、拒绝及低压诱导的家庭教养方式。2。

To an asymmetric synthesis of (1), we applied a type of Roush addition in the first step to introduce the C4 stereocenter, and then the chair substance -122 are readily transformed into the title compound -aphanorphine using the same route as to the racemic target molecular.

aphanorphine分子的不对称合成是在第一步通过对甲氧基苯乙醛（119）发生Roush反应引入手性，得到手性高烯丙醇-122，然后利用与合成外消旋目标分子相同的方法，光学纯化合物-122经一系列转化可得到光学纯中间体化合物139；化合物139发生分子内傅—克反应的过程中，由C4位手性立体特异地控制C1位手性的生成，从而解决-aphanorphine分子中的手性问题。

Numeration data between the groups using chi-square test.Results:Clinical research:(1) Baseline data: Except for gastrointestinal sthenia syndrome andyin/yang syndrome, the two groups of patients in terms of gender, age, smoking,alcohol consumption, medical history, diseased region, NIHSS score, strokeTCM symptom integration and Gelashige Coma Scale score, as compared withno statistical difference (P＞0.05);(2) After treatment: Compared with pretherapy, the NIHSS score and strokeTCM symptom integration of two groups were prominently improved at 14±3days and 21±3 days. But the improvement of stroke TCM symptom integrationof TFXSJN group was superior to that of the control group, the difference wasstatistically significant (P＜0.05);(3) At 21±3 days, TFXSJN group\' s BI Index, SS-QOL score were higher,and NIHSS score were significantly lower than that of control group, withsignificant differences (P＜0.05); There were no significant differencebetween two groups\' score of mRS at 21±3 days (P＞0.05);(4) Adverse reactions:All patients in the two groups during the treatmenthad no adverse reaction.

结果：临床研究：(1)基线资料中，除腑实证及阴阳类证外，两组患者在性别、年龄、吸烟、饮酒、既往病史、病变部位、NIHSS评分、中风中医症征积分及哥拉斯哥昏迷量表评分等方面相比无统计学差异，具有可比性(P＞0.05)；(2)治疗后，两组患者在14±3天，21±3天时的NIHSS评分、中风中医症征积分较治疗前均有所改善(P＜0.05或P＜0.01)，但TFXSJN组患者的中风中医症征改善优于对照组，具有统计学意义P＜0.05(来源：Ae7B9c9cC论文网www.abclunwen.com；(3)21±3天时TFXSJN组患者的BI指数、SS-QOL评分均高于对照组，NIHSS评分明显低于对照组，差异具有显著性(P＜0.05)；21±3天两组的mRS评分无明显差异(P＞0.05)；(4)不良反应：两组患者在治疗期间均未出现明显不良反应。

The water/metal fuel motor system configuration is based on a numerical simulation of combustion.

水反应金属燃料发动机[1]是一种全新概念的水下高能量密度推进系统，其工作原理为：雷外海水在速度头作用下进入燃烧室，与燃烧室中以一定形态存在的金属燃料发生强烈放热反应，产生的热量使未参与反应的海水受热蒸发，混合高压气体通过发动机喷管膨胀做功产生推力，从而推动水下航行

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了