外-斐反应

Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测，同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

Twenty five patients with gastritic carcinoma were vaccined with dendritic cells pulsed with tumor antigen and T cell subsets of peripheral blood, Th1 type and Th2 type (IL-4) cytokines of these patients serum were detected at different time point.

第三部分，从外周血诱导制备DCs疫苗，皮下注射治疗25例胃癌患者，初步观察DCs疫苗治疗前后胃癌患者迟发性超敏反应，T淋巴细胞亚群，NK细胞，外周血清IFN-γ、IL-4的水平及外周血PBMC在自体肿瘤抗原刺激下分泌IFN-γ、IL-4的能力。

Process for the preparation of phosphatidylserine of formula wherein R and R independently represent a saturated, mono-unsaturated or polyunsaturated acyl C10-C30, X=OH or OM where M=alkaline or alkaline earth metal, ammonium, alkylammonium including the transphosphatidylation reaction between phosphatidylcholine of the general formula wherein R and R and X have the above specified meanings, R=CH2-CH2- NH2 o CH2-CH2-N(CH3)3 and Serine in D, L or racemic form catalysed by the phospholipase D enzyme, characterised in that said reaction is carried out in a hydroalcoholic medium containing an aliphatic alcohol and in the presence of bivalent metal oxide.

本发明涉及制备式CH 2 OR 1 CHOR 2 CH 2 O-P-OCH 2 -CH(NH 2 )-COOHX的磷脂酰丝氨酸的方法，其中R 1 和R 2 独立地表示饱和的、单不饱和的和/或多不饱和的酰基C 10 -C 30 ，X＝OH或OM，其中M＝碱或碱土金属、铵、烷基铵，该方法包括通式CH 2 OR 1 CHOR 2 CH 2 O-P-OR 3 X的磷脂酰胆碱其中R 1 和R 2 以及X具有上述特别的含义，R 3 ＝CH 2 -CH 2 -NH 2 或CH 2 -CH 2 -N (CH 3 3 与D、L或外消旋形式的丝氨酸之间的转磷脂酰化反应，该反应是通过磷脂酶D酶催化的，其特征在于所述的反应是在包含脂族醇的含水醇介质中并且在二价金属氧化物的存在下进行的。

In gas phase, there are two reaction paths, the concerted and stepwise mechanisms, both of which produce the experimental product, hydroxamic acid; the stepwise mechanism is predominant.

在气相中均找到两条反应通道，即协同机理和分步机理，均生成实验产物氧肟酸，而且分步机理均为优势通道；除2-甲基-2-亚硝基丙烷的反应没有协同途径外，在溶剂中反应机理与气相中的类似。

In the distillation column with a single reactor, the outer loop reactor is designed and applied. The reaction dynamics datas of photochlorination of acetic acid in the reactor were tested. According to parallel-consecutive reaction dynamics model, the parameters were fitted and the model result tallys experiment result well.

在背包式反应精馏装置中，采用了外环流反应器，实验测定了该反应器中乙酸光氯化的反应动力学数据，采用平行-连串反应动力学模型进行了参数拟合，所得模型计算值和和实验值吻合较好。

Methods: Integrated and disrepaired skins in the rats were smeared with Salicylic AcidPhenol Liniment by the maximum tolerated dose only. The toxic reaction of the animals were observed and recorded continuously from the beginning of using the drug to 7 days, and evaluated by using the general standard of the reaction of skin stimulation in pharmacology. Animals were executed by taking off acanthi and blood was collected to check up the periphery blood routine and serum biochemistry at the end of the observation of the rats. And after the dissection, the pathological change of main visceras, such as heart, brain, liver and kidney were observed with naked eye and pathological slices. The data of this experiment was used to calculate the value of LD50 by using Bliss rule.

观察大鼠完整皮肤及破损皮肤一次性涂抹最大耐受量的小儿水杨酸苯酚搽剂所产生的毒性反应情况，从给药0时起至7 d连续观察记录动物的急性毒性和45 d连续的长期毒性反应，以皮肤刺激反应标准进行评分作毒理等级评价；对存活动物在观察结束时进行高位脱椎处死采血检测外周血常规和血清生化，解剖后裸眼和病理切片观察心、脑、肝、肾等主要脏器的病理变化。

Extracellularly recording the responses of dLGN neurons to sinusoidal gratings with different orientations showed that optimum orientations of dLGN neurons in trained cats, compared with that in untrained cats, did not shift in favour of the trained orientation. For cells with receptive fields located within 15°visual acuity away from the foveae, there are no difference in orientation bias and firing rate at trained orientation between the trained cats and untrained cats.

细胞外记录外膝体背核神经元对不同方位正弦光栅刺激的反应显示，垂直或水平方位正弦光栅信号训练的猫，与正常猫相比，其外膝体细胞的最优方位并未向着训练方位发生明显改变，对于感受野位于中央区15 度视角以内的细胞来说，其方位选择性强度以及在训练方位的发放强度与正常猫无明显差异。

The results showed that Berbamine could significantly inhibit the proliferative responses of murine spleen cells to Con A and Lipopolysaccharide reduce the amounts of hemolytic plaque forming cells of murine spleen cells and also decrease the delayed- type hypersensitivity and mixed lymphocytes reaction induced by allogenic antigen.

结果发现小蘖胺能在体内外抑制小鼠脾细胞对刀豆蛋白 A 和脂多糖的增殖反应，明显减少鼠脾细胞所致的溶血空斑细胞数，减低同种异型抗原诱导的迟发型超敏反应和混合淋巴细胞反应。

Progesterone and #gamma#-aminobutyric acid could initiate the acrosome reaction in guinea pig spermatozoa,which was independent of extracellular Cl一in vdro,and a potentiation effect between progesterone and GABA was demonstrated.

在体外，孕酮和#gamma#-氨基丁酸可明显地引起豚鼠精子顶体反应，并且两者合并使用，对顶体反应的诱发有协同作用；这种反应不依赖于细胞外C1-。

The effect of the amount of solvent was not distinct. In the molybdenum blue- di-n-octyltin oxide system, besides the effect of the amount of catalyst, oxidant, phase transfer, solvent and the reaction time, the effect of reaction temperature on the synthetic reaction of styrene oxide was researched.

在钼蓝-氧化三丁锡体催化系中，除研究了溶剂用量、催化剂用量、氧化剂用量、相转移剂用量以及反应时间对氧化苯乙烯收率的影响外，还研究了反应温度对催化氧化苯乙烯反应的影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了