外-斐反应

Alkaline phosphatase reaction was negative except in the endothelia of the blood vessel.

碱性磷酸酶除血管内皮外，均呈阴性反应。

The application of midazolam is effective on anti-axiety, sedation and anterograde amnesia in orthopaedic surgery with epidural block and the dose of 0.075mgkg^(-1) is more effective and safe.

咪唑安定应用于硬膜外麻醉下行骨科手术的抗焦虑、镇静及顺行遗忘效果好，按0.075mgkg^(-1)给药效果满意且安全性高，不良反应少。

Fifiteen experiment animals were divided randomly into normal control group, 12h-post-trauma group and 36h-post-trauma group (5 each). Immunohistochemistry and image analysis techniques were used to measure the area, optic density value and integrated optic density value and integrated optic density value of Ngb immunoreactive products in primary somatosensory cortex (S1) and retrosplenial granular b cortex, which was located in the inner side and out side of traumatic brain injury cavity in coronal section of rat brain respectively.

采用自由落体硬膜外撞击方法建立脑外伤模型，15只实验动物分为正常对照组及脑损伤12、36h组，应用免疫组织化学和图像分析技术观察伤灶周围第一躯体感觉皮质区(S1)和压部后颗粒细胞b皮质区内Ngb免疫反应阳性信号面积、光密度和累积光密度的变化。

According to the diagnostic charater such as the presence or absence of extracellular oxidase of mat, the type of hyphae and its differentiation, the distribution of clamp connection, the color of hyphae and mat and occurrence of spore, the culture character of 60 kinds of polypores in Northeast China were studied.

根据菌落细胞外氧化酶检测反应、菌丝类型及分化、锁状联合分布、菌丝和菌落的颜色、产孢情况等特征，对中国东北针阔叶树60种主要多孔菌，进行了培养特性及鉴定分类系统的研究。

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid.

取小鼠外周血非贴壁的淋巴细胞，与靶细胞混合淋巴细胞培养，进行淋巴细胞增殖和反应性T细胞功能检测，并检测细胞培养液中IL-2、TNF-α和IFN-γ水平。

The important roles of peracetic acid are more illustrated in various special oxidations in various industrial fields and synthesis chemistry apart from the biocidal activity and disinfecting action of peractic acid.

指出过氧乙酸的重要作用除了其广谱杀菌能力外，更体现在合成化学与工业领域的各类特殊氧化反应中。

Methods Tyresine kinase genes of EGFR （exons 18, 19 and 21） were amplified by PCR technology, and sequenced and analyzed by Chromas software in 80 NSCLC patients.

方法应用聚合酶链反应，对80例手术切除NSCLC瘤组织EGFR基因的第18、19和21外显子片段进行扩增和测序，Chromas软件分析基因突变。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein， Ad-Math1-EGFP)前庭阶导入组，每组5只，实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl，对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater， CDM-CEP)、听性脑干反应阈值检测和游泳试验，评价前庭和耳蜗功能，然后将动物处死进行组织形态学观察。

To explore the reciprocal effects of host-tumor interaction, we developed a system to assess the gene expression patterns and the diversity of gene expression of ERK and PI3K pathway in CAOV3 human ovarian cancer cells and primary carcinoma-associated fibroblasts cocultured with each other for 48 hours with RT-PCR.

方法1。采用逆转录聚合酶链反应的方法分别检测交互作用48小时后的卵巢癌相关成纤维细胞与卵巢癌细胞中基因表达的改变，CAFs及CAOV3细胞外信号调节激酶通路的ERK，PI3K基因表达的变化；2。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。