复苏

And the more debt that's wrung out of the system, the stronger the overall recovery.

而且系统内的债务削减规模越高，美国经济总体复苏的力度就更强。

Adeona will now connect to the storage servers and retrieve the recovery information.

adeona现在连接到存储服务器和检索复苏的信息。

A total of 50 couples allogene rats were performed same proceeding like that.

供体经过肝素化后，在0～4℃条件下取心肺，并作适当修剪，最后在0～4℃条件下将心肺供体SD大鼠的主动脉与受体Wistar大鼠的颈总动脉行端侧吻合，并进行心肺复苏。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The anteroposterior chest film and an AP pelvis may provide information that can guide resuscitation efforts of the patient with blunt trauma.

前后位胸片及盆部平片可以对钝性伤的病人提供有用信息以指导复苏的进行。

Plasma levels of diamine oxidase and serum levels of interleukin-8(IL-8) were measured at different time points including before shock, 2h ,6h and 24h after successful resuscitation of shock. Ileum tissue at 5 cm away from approximal ileocecal portion was removed and the change of morphology , distribution of lymphocyte , count of PMN and villous height, width and surface area were examined and measured.

在休克前、复苏成功后2h、6h、24h 取外周血，测定血浆二胺氧化酶含量及血清白介素-8(IL-8)水平；取距回盲部近端5cm 处回肠组织进行光镜组织形态学观察，淋巴细胞分布检测和多形核中性粒细胞计数，并测量回肠绒毛高度、宽度和表面积。

One time the little naughty god was wounded by his own arrows at and suchburning love was awakened in him for the human maiden psyche that he disregarded the constant interference of his mother and plucked up his courage to beg zeus for justice.another famousstory where eros played an important part was the argonautic expedition.medea,daughter of king aeetes,was wounded by eros\'\' arrows,took jason\'\'s part in recovering the golden fleece and eventually became the hero\'\'s wife.

有一次这位淘气的精灵被自己的箭射中。对人间少女普赛克炽热的爱在他心中复苏，以致于他不顾他母亲的干预，鼓起勇气让宙斯给予公正评判。厄洛斯起了重大作用的另一个著名的故事是亚尔古英雄的远征。美狄亚，国王埃厄忒斯的女儿，被厄洛斯的神箭射中，和伊阿宋一起寻觅金羊毛，最后成为这位英雄的妻子。

Though sometimes he was blindfolded ,no man nor god,zeus himself included,was safe from his evils.at one time the little naughty god was wounded by his own arrows and suchburning love was awakened in him for the human maiden psyche that he disregarded the constant interference of his mother and plucked up his courage to beg zeus for justice.another famousstory where eros played an important part was the argonautic expedition.medea,daughter of king aeetes,was wounded by eros'''' arrows,took jason''''s part in recovering the golden fleece and eventually became the hero''''s wife.

尽管有时他被蒙着眼睛，但没有任何人或神，包括宙斯在内，能逃避他的恶作剧。有一次这位淘气的精灵被自己的箭射中。对人间少女普赛克炽热的爱在他心中复苏，以致于他不顾他母亲的干预，鼓起勇气让宙斯给予公正评判。厄洛斯起了重大作用的另一个著名的故事是亚尔古英雄的远征。美狄亚，国王埃厄忒斯的女儿，被厄洛斯的神箭射中，和伊阿宋一起寻觅金羊毛，最后成为这位英雄的妻子。

Though sometimes he was blindfolded ,no man nor god,zeus himself included,was safe from his evils.at one time the little naughty god was wounded by his own arrows and suchburning love was awakened in him for the human maiden psyche that he disregarded the constant interference of his mother and plucked up his courage to beg zeus for justice.another famousstory where eros played an important part was the argonautic expedition.medea,daughter of king aeetes,was wounded by eros'' arrows,took jason''s part in recovering the golden fleece and eventually became the hero''s wife.

尽管有时他被蒙着眼睛，但没有任何人或神，包括宙斯在内，能逃避他的恶作剧。有一次这位淘气的精灵被自己的箭射中。对人间少女普赛克炽热的爱在他心中复苏，以致于他不顾他母亲的干预，鼓起勇气让宙斯给予公正评判。厄洛斯起了重大作用的另一个著名的故事是亚尔古英雄的远征。美狄亚，国王埃厄忒斯的女儿，被厄洛斯的神箭射中，和伊阿宋一起寻觅金羊毛，最后成为这位英雄的妻子。此主题出自 king。的博客

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。