复性

Andrias davidianus ; alkaline phosphatase ; tissues and organs ; renatured electrophoresis

中国大鲵；碱性磷酸酶；酶原位复性电泳；组织器官

ABSTRACT］ Objective To investigate the surgical efficacy through anterior loosening and posterior fusion for irreducible atlantoaxial dislocation.

目的 探讨难复性寰枢椎脱位经颈前路松解、后路融合的临床疗效。

Objective To investigate the surgical efficacy through anterior loosening and posterior fusion for irreducible atlantoaxial dislocation.

目的 探讨难复性寰枢椎脱位经颈前路松解、后路融合的临床疗效。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

The results of cellular-chemistry showed that the activity of AKP is mainly located in the membrane of liver cell especially more in the zone of canaliculi.

用酶的原位复性电泳测得小鼠生长过程中共出现四条AKP同工酶带，各同工酶活性随着小鼠的生长发育时间不同而发生变化。

PDGF-BB is the most effective subtype in the PDGF family on the metabolism of osseous tissue or the treatment of wound healing, and its clinical application was approved firstly among the growth factors although it was used to treat the decubital ulcer of diabetes.

本研究通过分子克隆方法获取人血小板衍生生长因子B链成熟肽基因，构建原核表达载体；利用大肠杆菌体系原核表达人血小板衍生生长因子B链前体蛋白，并纯化、复性进而获得具有功能活性的PDGF-BB；并对获得的重组rhPDGF-BB蛋白进行体外功能活性鉴定，同时，初步探讨PDGF-BB在毕赤酵母中的表达，为寻找更优化的表达纯化方法提供实验学依据。

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

The active form of NGF is a dimer in which two monomer is linked by 3 disulphide bonds. This form of dimer makes the purification of NGF difficulty because the protein expressed in E.

在蛋白纯化过程中这种由二硫键形成的空间结构往往是蛋白纯化过程中复性的难点，尤其是在大肠杆菌表达生物蛋白时，所表达的蛋白往往以无活性的单体形式存在于细胞内。

Further purification was performed on a column of Q-sepharose FF, and the 50mM elute contained the active DM650 with a purity of about 95%.

复性后的蛋白用Q-Sepharose FF离子交换层析进行纯化，50mM NaCl洗脱峰中含有目的蛋白，纯度可达95％。

The guanine plus cytosine content mol% of Pseudomonas cocovenenans subsp farinofermantans, Burkholderia gladioli and Burkholderia cocovenenans, which were similar in biochemical characteristics, were determined with Tm values method and HPLC method, and the comparative study of these two methods was discussed.

利用复性速率法和高效液相色谱法对生化性状比较相近的椰毒假单胞菌酵米面亚种、唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌共7个菌株的鸟嘌呤和胞嘧啶摩尔百分含量%进行了测定，并对这2种方法做了对比研究。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐