复性

How to refold inclusion body proteins with many pairs of disulfide bonds into natural conformation with high efficiency is a challengeable problem at present, and it is becoming one of the industrial bottlenecks in bioengineering field.

如何对富含二硫键的包涵体蛋白进行高效的体外复性使其获得正确的空间结构是重组蛋白生产所面临的巨大挑战，现已成为制约生物工程技术产业化的瓶颈问题之一。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

We have demonstrated that the renatured Escherichia colioverexpressed RpoⅠ can reconstitute with Rhizobium core RNA polymerase in vitro. A preparation procedure has been established, which is expected to be useful to further functional study.

我们证明了大肠杆菌表达变复性的RpoⅠ能够与根瘤菌的核心RNA聚合酶在体外重组成全酶，并建立了成熟的制备方案，为以后阐明RpoⅠ的功能奠定了基础。

In this thesis, ribonuclease A was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. On this basis, the refolding mechanism in vitro was discussed.

本文以核糖核酸酶A为蛋白质体外重折叠的模型蛋白，对其体外重折叠过程进行了研究，探讨了这类目标蛋白复性过程的一些规律性的问题。

Since the famous Anfinsen's experiment on enzyme ribonuclease, it is widely accepted that the correct three-dimensional conformation of a protein is contained in its primary amino acid sequence.

自从1956年著名的Anfinsen的牛胰核糖核酸酶的复性实验以来，人们普遍认为：蛋白质的三级结构唯一的由其氨基酸序列决定。

To construct the recombinant plasmid of protein 38kDa of M. tuberculosis and express, purificate and renaturate fusion protein 38kDa and to evaluate its potential value for TB serodiagnosis.

目的 构建结核分枝杆菌38kDa的重组质粒，在大肠杆菌中表达、纯化和复性重组蛋白，并评价其在结核病血清学诊断方面的价值。

SDS-PAGE analysis and Tofspec mass spectrography show that the molecular weight of rhBMP-2 is about 12kD.

本研究首先建立了较完整的rhBMP-2包涵体蛋白的分离、纯化和体外复性方法。

Following purification by Ni affinity chromatography and stepwise renaturation by dialysis, rB7-B5 showed sound antimicrobial activity in porcine actinobacillus pleuropneumoniae and antibiotics-resistant E. coli. The present study provides basis for the research and development of novel antibacterial preparations.

以经Ni亲和层析柱纯化和多步透析法复性的rB7-B5，对猪胸膜肺炎放线杆菌和耐药性大肠杆菌具有很好的抑菌活性，本研究为新型抗菌制剂的研制和开发奠定了基础。

AKP in twenty-four kinds of tissues and organs of Andrias davidianus were analyzed by the renatured electrophoresis.

用酶的原位复性电泳方法分析了中国大鲵二十四种组织器官的碱性磷酸酶。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐