复性

The result showed that NS1 protein was highly expressed in a way of inclusion body.

用HisTrap HP Kit进行复性和纯化，获得了纯度较高、生物活性较好的NS1蛋白。

So 30℃ is selected in this study to avoid the renaturation process of inclusion body and also could get more protein products.

选择30℃为诱导温度既避免了产物变性后所必需的复性环节，又能获得相对较高的生物量。

The results showed that P51 was expressed as inclusion body, and represented about 50% of total cellular protein. After purification and renaturation, the purity of P51 was up to 95%.

结果显示P51以包涵体的形式表达，表达量占菌体总蛋白的50％，经两步层析和透析复性，目的蛋白纯度大于95％。

The recombinant plasmid GST-STAT3-4 which is a truncation of STAT3 was transformed into E. coli JM109 and then induced to express a fussion protein by IPTG successfully. and we also renatured the intranuclear indusions of the fussion protein GST-STAT3-4

在实验的第二部分中我们成功的将STAT3的一个截断体STAT3-4的融合蛋白GST-STAT3-4在大肠杆菌中做了表达，并对以包涵体形式存在的STAT3-4蛋白进行了复性。

Objective:To explore a new method to treat the irreducible atlantoaxial dislocation.

目的：探讨难复性寰枢关节脱位的手术治疗方法。

After dissolved into 0.3％SKL anddialyzed for renaturation, the confluent protein had bioactivity of promoting porcineperipheral blood lymphoblast proliferation.

融合蛋白经过0.3％SKL溶解，稀释透析复性后，具有一定的促猪外周血淋巴母细胞增殖反应活性。

Objective To establish the high-cell-density fermenting method of recombinant human lymphotoxin alpha in Escherichia Coli and the annealing and purification methods of objective protein,then optimize the methods and obtain high purity rhLT-α.

目的建立和优化重组人淋巴毒素α工程菌高密度发酵方法以及目的蛋白的复性和纯化方法，得到高纯度的重组人淋巴毒素α。

Lymphotoxin Alpha ; High-Cell-Density Fermentation ; Annealing ; Purification

淋巴毒素α；高密度发酵；复性；纯化

The retention mechanism of the reduced and denatured Lys on the WCX column was proved to be a mixed mode of ion-exchange and hydrophobic interaction.

研究了还原变性Lys在三种疏水性不同的HIC柱上的复性情况。

With the denaturation and protein refolding, we gained the recombinant PlyC. To determine its biological activity, we adopted turbidimetry and plate count method. Before and after lysin treatment, the cell morphology was studied by scanning electron microscopy.

用透析复性方法得到目的产物重组链球菌噬菌体裂解酶PlyC，以浊度法和平板计数法检测其体外抗菌效果，扫描电子显微镜观察裂解酶作用前后细菌细胞形态变化。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐