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Some kinds of molecular chaperone systems and their functions as well were reviewed in this paper.

而利用分子伴侣的协助折叠特性进行的蛋白质复性技术正成为这一领域的研究热点。

In such computation, the whole sequence DNA molecules are involved in every operation of denaturalization and re-denaturalization, hence the number of experiment steps is important to the computing results of model.

在这样的计算中,每一步变性与复性都涉及到整个序列的DNA分子,因而操作步骤的次数对该模型的计算效果显得尤为明显。

A recombinant protein of Bla g 2 has been obtained, which is soluble in the supernatant and therefore can avoid a process of denaturalization and renaturation of the recombinant.

获得了真核表达的德国小蠊主要变应原重组蛋白Bla g 2,该蛋白以可溶性蛋白的形式分泌到培养液中,避免了原核表达的变复性过程。

When initial lysozyme concentration in denaturant solution was more than 10 g/L, the denatured lysozyme molecules were renatured and isolated in a size exclusion chromatographic column.

在体积排阻色谱柱上研究了还原剂存在时脲和盐酸胍变性的3种溶菌酶溶液的复性和分离过程。

ABSTRACT Protein folding remains a bottle-neck issues in the down-stream technology in biotechnology.

基因重组蛋白的复性问题是生物工程下游技术中的一个瓶颈。

Having been renaturalized, the fusing protein product is digested with enterokinase, purified by Ni-sepharose and HPLC.

复性、肠激酶酶切、Ni-Sepharose和HPLC纯化后,用质谱测定其分子量并进行活性测定。

In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.

此外类凝血酶基因r8在pBAD/TOPO中也得到高效融合表达,表达产物经酶切、纯化和复性,测得精氨酸酯酶活性。

Equilibrate the column with 5 ml distilled H2O and 3 ml diluted binding buffer (Fig 2a).

用5毫升蒸馏水和3毫升稀释过的复性缓冲液调和柱床如图

Purpose: Study on the refolding of inclusion body of recombinant human granulocyte colony stimulating factor.

研究人粒细胞集落刺激因子包涵体的复性方法。

The renaturation methods of inclusion body proteins dissolved by HAC or urea are explored.

分别对乙酸溶解的包涵体蛋白和尿素溶解的包涵体蛋白的复性方法进行了摸索。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。