复性

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

By using lysozyme as model protein, this equation was tested with the renaturation process of three kinds of denatured lysozyme molecules in urea and guanidine hydrochloride solutions, and it was found that the renaturation results of denatured lysozymes could be described well by this equation, and these denatured lysozyme molecules had a tendency to form a bi-molecular intermediate aggregate in both solutions.

以三种溶菌酶在脲和盐酸胍溶液中的变性-复性过程对此方程进行了验证，结果表明所给出的方程能够很好地描述三种溶菌酶在这两种变性液中的复性结果，三种溶菌酶在两种变性液中有形成二分子集聚体的趋势。

This equation was tested by the renaturation processes of denatured egg white lysozyme in guanidine hydrochloride and urea solutions, with the results to show that when guanidine hydrochloride and urea concentrations were separately higher than 1.25 and 3.00 mol/L or separately lower than 1.00 and 3.00 mol/L, the refolding intermediates of egg white lysozymes were more easily aggregated to aggregate state or more easily renatured to native state, respectively.

以蛋白溶菌酶在盐酸胍和脲溶液中的复性过程对此关系进行了验证，结果表明，当溶液中盐酸胍和脲浓度分别大于1.25 mol/l和3.0 mol/l或分别小于1.0 mol/l和3.0 mol/l时，它们的折叠中间体分子分别更倾向于集聚成集聚体或更倾向于复性成原始态分子。

The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography.

对包涵体的复性条件进行了摸索，建立了IFN BLA的复性及纯化方法，纯化后的蛋白产量约为45mg/L，纯度在90%以上。

The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography. The overall yield of IFN-BLA was about 45 mg/L with purity higher than 90%.

对包涵体的复性条件进行了摸索，建立了IFN-BLA的复性及纯化方法，纯化后的蛋白产量约为45 mg/L，纯度在90％以上。

According to structure analysis, the contents of α-helix and dimer structure in refolded eMDH were increased by the cooperation, and eMDH activity was enhanced.

对复性产物结构的初步分析发现，耦合作用可使eMDH分子的α-螺旋及二聚体含量明显增多，因此复性产物的活性也显著提高。

The results showed that the purified recombinant protein under denaturing conditions was refolded in Ni-NTA column by using refolding buffer.

实验结果表明，在变性条件下纯化的样品在柱上不经洗脱，直接用复性液洗涤6h，可以使EKLC在得到提纯的同时实现复性，得到了电泳纯度为96％的具有生物活性的EKLC，最高活性为712U。

Therefore the denatured protein could pass along the gradient and refolded to native conformation as urea was gradually removed and a linear removal of urea was realized in this manner.

在与常用复性方法的比较中，这种方法在模型蛋白质溶菌酶的复性实验中初步显示出了一定的优越性。

It was found that SEC can not improve the kinetics of refolding yet.

但它与其它吸附模式的色谱方法一样，能够提高复性产率，但并不能加快复性的动力学。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐