复性

By using a simple, novel inclusion body purification method, high efficiency of refoking of rhG-CSF is attained.

对rhG－CS F包涵体提纯和复性的研究，建立了rhG－CSF的复性新方法。

The flow rate of mobile phase was not found to have any important effects on the bioactivity yield of Lys.

但也并不能加快复性动力学，流速的快慢对其复性的最终效果并没有大的影响。

Refolding of reduced/denatured Lys was compared between using SEC and adsorption chromatography.

比较了还原变性Lys在SEC色谱柱上的复性，认为SEC是一种较好的色谱复性方法。

Because it is a hard work to refold the denatured inclusion proteins correctly, especially for those which contain several disulfide bonds. the results?repeatability of some activities is not very perfect.

纯化包涵体后，将产物变性成可溶形式，然后用Ni-NTA对表达产物纯化，SDS-PAGE检测为一条带，包涵体的复性是一项非常困难的工作，尤其是对含有多对二硫键的蛋白质，因此复性后冻干，测它的某些活性时，结果的重复性不太理想。

The inclusion bodies were solubilized in 50mM Tris-buffer at pH 12.5 and then were diluted to refold the denatured proteins. Refolding of D-hydantoinase by direct and fed-batch operations was investigated . Furthermore, the influence of the additives, CTAB and MnCl2, for soluble protein recovery was also studied.

实验中先以pH 12.5， 50mM Tris-bufferru将包涵体溶解，再藉由稀释法使其复性，并探讨直接稀释法与馈料稀释法对乙内醯尿酶复性的影响，另外探讨添加剂是否对可溶蛋白回收率有所帮助。

The oxidative refolding of denatured-reduced lysozyme in the presence of cetyl trimethyl ammonium bromide and/or β-cyclodextrin was investigated.

研究了β-环糊精和表面活性剂十六烷基三甲基溴化铵作为复性液添加剂对促进变性-还原溶菌酶的氧化复性。

Results Among the pre-schoolers,the hyperopia is primarily, specially the complex hypermetropic astigmatism. While among the school-age teenagers,myopia increases obviously,and the complex myopic astigmatism are most.

结果 学龄前儿童以远视眼为主，特别是复性远视散光最为多见，学龄期青少年儿童近视眼明显增多，其中复性近视散光最多。

The organization of genomes of A. Sinensis and A. Mississippiensis have been examined, Both reassociation curves are very similar. There are only small fractions of highly repetitive sequences in genomes.

通过DNA复性动力学的分析，测定了扬子鳄和密河鳄基因组的组织结构，两者的复性曲线非常想象，高度重复DNA含量很少，而中度重复DNA序列各占基因组的30％左右。

Sodium lauroyl sarcosine was used as a denaturant, a novel protein refolding method, which was proposed on the basis of one step-AMC and IEC, one step-AMC-IEC was used on the refolding of denatured/reduced lysozyme.

一步人工分子伴侣-离子交换色谱法辅助还原变性溶菌酶的复性：以月桂酰基肌氨酸钠(Sodium Lauroyl Sarcosine，Sarkosyl，SLS)作为蛋白变性剂，发展了一步人工分子伴侣-离子交换色谱法，对还原变性溶菌酶进行了复性研究。

It has also been discovered that, before complete purification, the synthetic HWTX-Ⅰ, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-Ⅰ that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-Ⅰ.

推测除了在合成HWTX-Ⅰ复性过程中有少数分子发生二硫键错配外，还有部分分子在固相合成中发生了消旋作用，需要交替采取多种色谱法才能完全分离纯化复性后的合成多肽产物。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐