复性

DM650 was expressed as inclusion bodies , and it must be dissolved in denatured condition and then renatured.

表达产物以包涵体形式存在，必须进行变性溶解和复性处理。

The protein purified with Ni-NTA column was denatured by 8 mol/L urea and dialyzed for refolding.

Ni-NTA柱纯化后的特异性蛋白经8 mol/L脲素变性，梯度透析使蛋白质复性。

METHODS: The VacA antigen expressed engineering bacterium was induced by lsopropyl-β-D-thiogalactopy-ranoside. The expressed inclusion body was denatured, renatured and dialyzed, and its activity was identified.

利用已经构建的表达VacA抗原的工程菌，用IPTG诱导，表达的包涵体通过系列条件的变性、复性、透析，并经活性鉴定。

PAO was purified by Ni-NTA resin under denature condition and dialyzed to recover its native structure. After that, the enzyme activity was analyzed with chemical fluorescent method.

IPTG 诱导表达的重组PAO经Ni-NTA亲和层析纯化和透析复性后，化学荧光法测定酶活性。

A small amount of soluble Trx-IPSE was obtained by dialyzing the fusion protein in a large volume of PBS.

用大量PBS对变性融合蛋白进行透析，获得部分复性的可溶性Trx-IPSE融合蛋白。

Formolongi was as follows: annealing for 50 s at 37℃, elongating for 60 s, recycling for 35 times.

麝香百合的最佳RAPD反应程序为：37℃复性50s，延伸60s，循环35次。

Results showed that HOmg inclusion bodies of recombinant pThr-2 were overproduced in E.coli BL21 (DE3) per liter broth, which increased about 40%.

同时也进一步验证了PNIPA凝胶协助复性主要是通过抑制蛋白质相互间的聚集反应来实现的。

Next, the oxidative refolding of denatured-reduced Lysozyme in presence of cetyltrimerthyl ammonium bromide and β-cyclodextrin were investigated.

接着研究了去污剂十六烷基三甲基溴化铵和β-环糊精的人工分子伴侣体系对变性-还原溶菌酶的氧化复性效果。

It is found that the best refolding yield for 0.1g/L linear HNTX-Ⅲ could be obtained in double-distilled aqueous solution at pH 7.5 containing 1.0 mol/L L-arginine, 1.0mmol/L reduced glutathione, and 0.1mmol/L oxidized glutathione.

最佳的氧化复性条件为pH 7.5的重蒸水溶液体系，样品质量浓度为0.1mmol/L，还原型谷胱甘肽和氧化型谷胱甘肽的浓度分别为1.0mmol/L和0.1mmol/L、L-Arg浓度为1.0mol/L。

The unfolded protein correctly refolded to the native conformation through the column, and was partly purified during the elution process.

其原理是将层析技术应用于蛋白质复性和纯化，使变性蛋白质在层析柱上折叠为正确的空间构象，在洗脱的同时实现部分纯化。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐