复性

Results Hypermetropia and hyperopic astigmatism were found in 85 percent of pterygium patients.

单纯近视及散光17眼（8.5%），复性远视散光6眼（3%），顺规散光2眼（1%），斜向散光1眼（0.5%），逆规散光眼1眼（0.5%）。196例（200眼）之中远视散光中散光轴位90°方位172眼（86%），180°方位21眼（10.5%），其他方位仅7眼（3.5%）。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达，包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性，以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

Under different initial total egg white lysozyme concentrations in urea solution, the refolding egg white lysozyme intermediates could be deduced to have a tendency to form a bimolecular intermediate aggregate, and this inference was further confirmed by their nonreducing SDS-PAGE and size exclusion chromatography.

当不同浓度蛋白溶菌酶在脲溶液中复性时，蛋白溶菌酶折叠中间体有形成二分子积聚体的趋势，这个推测得到了非还原电泳和体积排阻色谱结果的证实。

The optimum PCR reaction method were as follows: 95℃predegenerate 5min, 94℃degenerate 30s, 35℃Cregenerate 50s, 72℃elongate 90s, 40 circles in all, and the last circle maintain 5min at 72℃.

最适PCR反应程序为：95℃预变性5min，94℃变性30s，35℃复性50s，72℃延伸90s，共进行40个循环，最后一个循环在72℃保持5min。

And the amplification procedure was as follows: preparatorily denaturing for 3 min at 94℃, denaturing for 30 sec at 94℃, annealing for 30 sec at 37℃, elongating for 90 sec at 72℃, 41 cycles, and elongating for 7 min at 72℃.

扩增程序为：94℃预变性3 min，然后于94℃变性30 s，37℃复性30 s，72℃延伸90 s，循环41次，最后于72℃延伸7 min。

The enzyme reductively unfolded is almost unable to renature indicating the roles of disulfide bonds on maintaining the correct tertiary structure of the enzyme.

还原条件下变性的酶几乎不能复性，表明二硫键对于维持酶的特定空间结构是极为重要的。

An optimal refolding procedure has been developed to reford active proteins from inclusion bodies.

故欲发展出一个最适的复性程序，将包涵体回复成具有活性的蛋白质。

But 5-helix was apt to form inclusion body when expressed directly in prokaryotic cell and was difficult to renature, which causes inconvenience to future study.

但5-helix基因在原核细胞中直接表达时易形成包涵体，复性困难，给研究带来不便。

With ultrasonic wave and lysozyme, the recombination N protein is split from bacteria. We dissolve it in guanidine hydrochloride, purify it with Ni〓 affinity chromatography column and renature it in vitro. By testing the antigen of the purified N protein, it shows that its activity is very high.

建立了用超声波和溶菌酶裂解菌体，用盐酸胍溶解，Ni〓亲合层析柱纯化及体外复性等方法，经检测证明纯化的VSV重组核蛋白抗原具有较高的活性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐