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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

The Six Sigma Black Belt will know how to augment a factorial design to create a composite or central composite design.

六西格玛黑带应该了解如何将析因设计扩展为复合设计或中心复合设计。

It is discovered that metal protectant and polar polymer can reduce fretting wear of complex lithium grease largely by utilizing Fafnir friction oxidation tester investigates fretting wear of complex lithium grease .

用费夫纳摩擦氧化试验机考察复合锂基润滑脂的微动磨损时,发现金属保护剂和极性聚合物可大幅度降低复合锂基脂的微动磨损。

The PANI-C composite electrodes had better cycling stability than PANI electrodes and the specific capacitance of the composite electrodes was 587F/g, which was much higher than that of the pristine activated carbon (140F/g), owing to the faradic reaction of PANI with the electrolyte.

聚苯胺/活性炭复合电极比活性炭电极具有更高的容量,同时比聚苯胺电极具有更好的循环稳定性。聚苯胺/活性炭复合电极的比电容为587F/g,而活性炭电极仅为140F/g。

Results indicate that TiC-VC particles can be produced by direct metallurgical reaction between ferrotitanium, ferrovanadium and graphite during the laser cladding process.

研究结果表明,通过钛铁、钒铁与石墨之间的反应,可以得到TiC-VC复合碳化物增强相,细小的TiC-VC复合碳化物颗粒弥散分布在Fe基体之中,合金熔覆层的耐磨性在一定范围内随增强颗粒的增加而提高。

A kind of Fe-Ti-C composite powder for reactive spraying was prepared with ferrotitanium and sucrose as raw materials. TiC/Fe composite coatings were synthesized and deposited by reactive plasma spraying.

以钛铁粉、铁粉和蔗糖为原料,采用前驱体热分解复合技术制备了Fe-Ti-C系反应热喷涂粉末,并通过普通等离子喷涂技术原位合成并沉积了TiC/Fe金属陶瓷复合涂层。

This paper used anhydrite associated with ferruginous mineral from Cheng Chao iron ore as primary raw material to develop successfully a new type of light wall material under the action of G N M J four compound excitation system and FX compound reinforcing fibre with suitable forming procedure.

本研究以武钢矿业公司程潮铁矿共生的硬石膏为主要原料,在广泛探索的基础上,筛选出G-N-M-J四元复合激发体系,有效地激发了硬石膏的活性,在FX复合纤维的增强作用下,采用合适的成型工艺条件,成功地研制出一种新型的轻质墙体材料—纸面硬石膏板,对试制样品的测试结果表明,各项性能达到或接近以半水石膏为主要原料的纸面石膏板的国家标准,为综合利用程潮铁矿的矿物资源开辟了新的途径,对硬石膏在新型建筑材料领域中的开发应用,更有着开拓性的意义。

The results indicated that the soybean protein fibers have skin-core structure and fibril structure.

通过扫描电镜、光学显微镜等方法对大豆蛋白/PVA复合纤维的微细结构做了分析和比较,结果表明,大豆蛋白/PVA复合纤维有比较明显的皮芯结构,皮层厚度约为1。

The invention relates to an organic thin-film transistor of three-layer composite film insulated gate which comprises a substrate (1), a metal electrode (2), a organic semiconductor layer (6), a source/leakage electrode; and also comprises: a low dielectric constant polymer layer (3), a high dielectric constant oxide layer (4), a low dielectric constant polymer layer (5), three-layer composite film insulated gate is composed of the (3) layer, the (4)layer and the (5) layer.

本发明涉及三层复合膜绝缘栅的有机薄膜晶体管,包括衬底(1),金属电极(2),有机半导体(6)层,源/漏电极(7);还包括:低介电常数聚合物层(3),高介电常数氧化物层(4),低介电常数聚合物层(5),所述的(3)、(4)和(5)层构成三层复合膜绝缘栅。

Simulated using the ANSYS finite element software the quantity of heat when it transmits from the room to outdoor through the core board material under the winter condition.

采用稻草板与聚苯乙烯泡沫板复合作为复合墙体的保温材料,利用ANSYS有限元软件模拟冬季条件下热量由室内通过该保温材料向室外的传递。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。