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Objective To explore the effect of different anticoagulants on quantitative assays for HBV DNA using real-time PCR technique. Methods Peripheral blood samples were obtained from 40 patients infected with HBV. Serum and plasma samples from three kinds of anticoagulants such as heparin, trisodium citrate, and dipotassium EDTA were stored at -20℃.

目的 探讨用实时荧光定量PCR技术检测乙型肝炎病毒DNA拷贝时,不同抗凝剂对乙型肝炎病毒DNA定量检测的影响方法采集40例乙型肝炎患者血液,分别置于不含抗凝剂及含肝素钠、枸缘酸钠、EDTA-K2抗凝剂的无菌试管中,分离血清和血浆后于-20℃冰箱保存备用

Methods peripheral blood samples were obtained from 40 patients infected with hbv. serum and plasma samples from three kinds of anticoagulants such as heparin, trisodium citrate, and dipotassium edta were stored at -20℃.

采集40例乙型肝炎患者血液,分别置于不含抗凝剂及含肝素钠、枸缘酸钠、edta-k2抗凝剂的无菌试管中,分离血清和血浆后于-20℃冰箱保存备用

MineMaster OTR Tire , 1ST UK Manufactured/Distributed Giant OTR Tire , in radial and bias , warranty on each tyre , full liabilty insurance , certificate of working hours , 5000 guarnteed on radial , 3000 working hours on Bias , sizes from 2700 to 4000R57 , smaller bias available , available in heat and cut compounds , available on monthly contract , full back up service and customer after sales suport , 30-120 dyas credit available from shipment.

MineMaster工程机械轮胎,第一家英国制造和经销的巨型工程机械轮胎,子午线轮胎和斜交轮胎均有供给,每个轮胎都提供质保、全额责任保险、工作时间凭证,子午线轮胎确保5000小时,斜交轮胎为3000小时,尺寸从2700至4000R57。小型斜交轮胎可选,经加热和切割处理的踏面胶料可选,可按月签合同,提供全面的备用服务和售后客服,装运后提供30-120天信贷。

MineMaster OTR Tire , 1ST UK Manufactured/Distributed Giant OTR Tire , in radial and bias , warranty on each tyre , full liabilty insurance , certificate of working hours , 5000 guarnteed on radial , 3000 working hours on Bias , sizes from 2700 to 4000R57 , smaller bias available , available in heat and cut compounds , available on monthly contract , full back up service and customer after sales suport , 30-120 dyas credit available from shipment.

MineMaster工程机械轮胎,第一家英国制造和经销的巨型工程机械轮胎,子午线轮胎和斜交轮胎均有供应,每个轮胎都提供质保、全额责任保险、工作时间凭证,子午线轮胎确保5000小时,斜交轮胎为3000小时,尺寸从2700至4000R57。小型斜交轮胎可选,经加热和切割处理的踏面胶料可选,可按月签合同,提供全面的备用服务和售后客服,装运后提供30-120天信贷。

This text aim at the neutral spot not connect the ground or through eliminate arc coil to conncect ground of connecting a ground of system, put out to connect a ground of electricity arc ability existently low and starts of the system occurrence Human body feeling electricity connect the ground to conduct electricity to press, the equipments inner part breaks down to connect the ground, having no problem of protect the ability.On the foundation of the thorough research protection method, put forward to change into above-mentioned system the protection project that the intelligence connects a ground of system movement.At the same time, introduced the intelligence to connect a ground of composing of system, the work principle, function and characteristicses and system to circulate the circumstance.

本文简要分析了我国配电网自动化的现状和面临的主要问题,指出合理、高效的配电系统网络结构是提高供电可靠性和供电质量的前提条件,本文在对国内现有配网方式进行全面分析的基础上,结合我国城网改造的目标的显状,提出了一种科学&田&字型接线,对其技术性、经济性和可靠性进行了论证,(分析了不同故障情况下系统如何完成故障的定位、隔离,网络的重构及负荷的转移,指出该方案具有很高的供电可靠性和相对较低的备用容量,是一种符合国情国力并能适应配网自动化发展的组网方案);给出了适合该方案的不同控制模式并进行了优劣比较,指出集中远方控制模式划配网自动化发展的必然趋势;最后本文给出了该控制模式下的测控方式。

Or take a spare foldable bag that can be slipped into your luggage easily.

或采取备用折叠袋,可潜入您的行李轻松。

Is your genny up and running?

你们的备用发电机工作吗?

Anyone with a genny should go home immediately, just to be safe.

出于安全,有备用发电机的都待在家里

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤,将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮,再置入曲拉通X-100溶液使真皮细胞完全脱净,磷酸盐缓冲液反复清洗,即为脱细胞真皮基质,4 ℃保存备用

Cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤,将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮,再置入曲拉通X-100溶液使真皮细胞完全脱净,磷酸盐缓冲液反复清洗,即为脱细胞真皮基质,4 ℃保存备用

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。