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Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠,血清进行肝功能检测;苏木素-伊红染色部分肝组织;Trizol-酚-氯仿一步法提取小鼠肝组织总RNA,紫外分光光度法测定总RNA浓度及纯度,实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达,以18S基因作为质控。

Methods Twenty eight pure New Zealand rabbits were used, of which four were for nerve localization and the rest were randomized into untreatment group and ultrashort wave groups, after the establishment of the nerve root compression models by inserting a silastic tube into the intervertebral canal to compress the sixth and seventh left lumbar nerve roots.

方法纯种新西兰成年兔28只,4只作神经定位解剖,其余24只全部做手术造成腰神经慢性压迫,随机分成2组,未处理组和超短波组,每组又分10 d组、30 d组和180 d组。压迫模型:将硅胶管从椎间外孔缓慢塞入椎间管内,压迫腰6和腰7左侧神经根直到实验兔被处死,右侧为自身对照侧。

Al-Qaida leader Osama bin Laden threatened in a new audio recording released Thursday to kill any captured Americans if the US executes the self-professed mastermind of the Sept.11 attacks or any other al-Qaida suspects.

基地组织头目奥萨马·本·拉登在本周四公布的一段录音中威胁称,如果美国处死其所认定的9·11恐怖袭击幕后主谋或涉嫌制造该事件的基地组织其他成员,他就将处决所有被囚禁的美国人。

On the 10th day and on the 30th day,quickly took out Livers of mice,fixed in the Zamboni fixing solution,conventionally produced frozen section and stain section by immunohistochemical technology.

分别于第10天和第30天时处死各组小鼠,快速取出肝脏,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。

On the 10th day,quickly took out testis of mice,fix in the Zamboni fixation solution,conventionally produce frozen section and stain section by immunohistochemical technology.

健康雄性昆明小鼠30只,随机分为对照组、1%NP组和1‰NP组。10d后处死各组小鼠,取出睾丸,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。

The control group were injected peritoneal physiological saline injection.Three study groups were injected acrylamide solution 5 mg.kg-1.d-1,30 mg.kg-1.d-1,and 60 mg.kg-1.d-1,respectively.On the 5th day,brains of each group were taken out quickly,fixed by Zamboni fixation solution,made into conventional freezing moviemaking,stained by immunohistochemistry staining,and then observed by light microscope.

对照组小鼠腹腔注射生理盐水;实验组小鼠分别代以5 mg·kg-1·d-1、30 mg·kg-1·d-1、60 mg·kg-1·d-1丙烯酰胺水溶液腹腔注射。5 d时分别处死各组小鼠,取出大脑,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。

Kg1?d1 orally since the age of 8 weeks and the rats in the other two groups were given distilled water orally. body weights of rats were recorded weekly and blood glucose concentration was determined by oral glucose tolerance test. pancreas weights were recorded after rats were killed and the expression levels of bcl2 mrna and bax mrna were detected by reverse transcriptasepolymerase chain reaction.

每周记录大鼠体质量;采用口服葡萄糖耐量试验监测血糖;定期处死大鼠,分离胰腺并称重;采用逆转录聚合酶链反应检测bcl2和bax在胰腺组织中的表达。

Methods : We gave 0, 1, 2, 3, 4 times low dose total-body irradiation to control group (No. 1 group) and experiment group (No. 2, 3,4,5 group) respectively with γ irradiation .Each dose is 0. 1GY and dose rate is 1.28 cGY/min. The interval of each irradiation is 48 hours . Six hours after every group finished their own total dose , we kill the rats and get their thyme .

采用γ射线分别对对照组(1组)和实验组(2、3、4、5组)给予0、1、2、3、4次低剂量全身照射,单次剂量0.1GY,剂量率为1.28cGY/min,每次照射间隔时间48小时,各组在完成照射总剂量后6小时处死取胸腺,采用双参数直接免疫荧光流式细胞术,检测大鼠低剂量全身照射后胸腺细胞CD4、CD8、CD3~+T细胞的表达情况以及CD4/CD8的变化。

Their offsprings' body weight, body length, tail length and the time of auricle separation, teeth burgeon, eye opening, ear hole opening, hair appearance were measured. Then 64 offsprings (8 each group, half female and half male) were killed when they were 70-day-old and the same indexs as pregant rats' were measured.

另一半孕鼠正常分娩,仔鼠出生后测量体重、身长、尾长,观察耳廓分离、门牙萌出、睁眼、张耳及出毛情况;70天龄时称重并处死64只仔鼠,每组8只,雌雄各半,分别测定脏器重量、血清生殖激素、甲状腺激素和免疫学指标。

In 1997, the remains of revolutionary Ernesto "Che" Guevara were laid to rest in his adopted Cuba, 30 years after his execution in Bolivia.

1997年,在玻利维亚处死后30年,革命烈士Ernesto &Che& Guevara 的遗体运回曾经生活过的古巴。

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